SNP Miniplexes for Individual Identification of Random‐Bred Domestic Cats |
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| Authors: | Ashley Brooks BS Erica K Creighton BS Barbara Gandolfi PhD Razib Khan BS Robert A Grahn PhD Leslie A Lyons PhD |
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| Affiliation: | 1. Department of Population Health and Reproduction, School of Veterinary Medicine, University of California – Davis, Davis, CA;2. Department of Veterinary Medicine and Surgery, College of Veterinary Medicine, University of Missouri – Columbia, Columbia, MO |
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| Abstract: | Phenotypic and genotypic characteristics of the cat can be obtained from single nucleotide polymorphisms (SNPs) analyses of fur. This study developed miniplexes using SNPs with high discriminating power for random‐bred domestic cats, focusing on individual and phenotypic identification. Seventy‐eight SNPs were investigated using a multiplex PCR followed by a fluorescently labeled single base extension (SBE) technique (SNaPshot®). The SNP miniplexes were evaluated for reliability, reproducibility, sensitivity, species specificity, detection limitations, and assignment accuracy. Six SNPplexes were developed containing 39 intergenic SNPs and 26 phenotypic SNPs, including a sex identification marker, ZFXY. The combined random match probability (cRMP) was 6.58 × 10?19 across all Western cat populations and the likelihood ratio was 1.52 × 1018. These SNPplexes can distinguish individual cats and their phenotypic traits, which could provide insight into crime reconstructions. A SNP database of 237 cats from 13 worldwide populations is now available for forensic applications. |
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| Keywords: | forensic science forensic genetics animal forensics
Felis silvestris catus
single base extension single nucleotide polymorphism |
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