| 桃红四物汤干预后的血小板外泌体对脑缺血再灌注模型大鼠的血管新生作用 |
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| 作者姓名： | 武 瑞 戴文涛 张亚南 侯欣宇 彭代银 韩 岚 |
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| 作者单位： | 1.安徽中医药大学药学院，安徽 合肥 230012；2.中药复方安徽省重点实验室，安徽 合肥 230012；3.安徽道地中药材品质提升协同创新中心，安徽 合肥 230012 |
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| 基金项目： | 国家自然科学基金项目（8207142977） |
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| 摘 要： | 目的 探究桃红四物汤(Taohong Siwu Decoction,THSWD)干预后的血小板外泌体（platelet-derived exosomes，PLT-Exos）对脑缺血再灌注大鼠的血管新生作用。方法 采用超高速差速离心法提取PLT-Exos，透射电子显微镜观察外泌体形态,纳米微粒示踪分析检测外泌体粒径分布,蛋白质免疫印迹法检测外泌体标志蛋白表达水平；建立大鼠脑缺血再灌注模型，将所提取的经THSWD干预后的PLT-Exos通过尾静脉注射到模型大鼠体内，再通过脑血流量测定、苏木精－伊红染色、免疫荧光染色，验证THSWD-PLT-Exos对大鼠脑缺血再灌注模型血管新生的治疗作用。结果 提取的PLT-Exos表征符合典型。THSWD-PLT-Exos可显著提升脑缺血大鼠脑部的血流量，改善海马区损伤，具有良好的血管生成和神经保护作用。结论 THSWD-PLT-Exos对脑缺血再灌注大鼠损伤有良好的血管新生作用。
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| 关 键 词： | 桃红四物汤 血小板外泌体 脑缺血再灌注损伤 血管新生 |
Effect of Platelet-derived Exosomes after Intervention with Taohong Siwu Decoction on Angiogenesis in a Rat Model of Cerebral Ischemia/Reperfusion |
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| Affiliation: | 1. School of Pharmacy, Anhui University of Chinese Medicine, Anhui Hefei 230012, China; 2. Key Laboratory of Chinese Medicinal Formula of Anhui Province, Anhui Hefei 230012, China; 3. Synergetic Innovation Center of Anhui Authentic Chinese Medicine Quality Improvement, Anhui Hefei 230012, China |
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| Abstract: | Objective To investigate the effect of platelet-derived exosomes (PLT-Exos) after Taohong Siwu Decoction (THSWD) intervention on a rat model of cerebral ischemia/reperfusion. Methods PLT-Exos were extracted by ultra-high speed differential centrifugation; a transmission electron microscope was used to observe the morphology of exosomes; nanoparticle tracing was used to observe the particle size distribution of exosomes; Western blot was used to measure the expression of exosome biomarkers. A rat model of cerebral ischemia/reperfusion was established, and PLT-Exos extracted after THSWD intervention (THSWD-PLT-Exos) were injected into the model rats via the caudal vein; cerebral blood flow measurement, HE staining, and immunofluorescent staining were used to verify the therapeutic effect of THSWD-PLT-Exos on angiogenesis in a rat model of cerebral ischemia/reperfusion. Results The PLT-Exos extracted had typical characteristics. THSWD-PLT-Exos significantly increased cerebral blood flow, improved hippocampal injury, and had good angiogenesis and neuroprotective effects. Conclusion THSWD-PLT-Exos has a good angiogenesis effect on a rat model of cerebral ischemia/reperfusion injury. |
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| Keywords: | Taohong Siwu Decoction Platelet-derived exosome Cerebral ischemia/reperfusion injury Angiogenesis |
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