共查询到19条相似文献,搜索用时 64 毫秒
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目的建立液相色谱分析方法,对市售的30种不同厂家牌号(原装)染料型黑色喷墨打印字迹进行区分鉴别。方法通过选择最佳的提取条件、流动相,考察纸张、字迹形成方式等的影响,及样品及仪器的稳定性实验,确定实验条件。结果选择了最佳的反相高效液相色谱(RP-HPLC)分析方法:固定相为X-bridge Shield RP18(3.5μm,4.6×100mm),流动相为乙腈-NH4HCO3溶液(10mmol/L),梯度洗脱,检测波长为585nm、475nm;用该方法,根据字迹染料成分的差异将30种样品分为四大类17小类。结论该方法可以为鉴别区分不同厂家染料型黑色喷墨打印字迹提供依据。 相似文献
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本文报道反相高效液相色谱法测定全血中利多卡因浓度的方法。氟安定作为内标,全血经溶血,1N 氢氧化钠1ml 碱化后,用乙醚提取。以Zorbax ODS 为固定相,以CH_3 OH∶H_2O∶(C_2H_5) _3N(100∶45∶0. 2,pH6. 3) 为流动相,检测波长为240nm。用利多卡因为内标的峰高比定量。血中利多卡因最低检出浓度为0. 125ug/ml。线性范围是0. 0025~0. 0750mg/ml(r=0. 9999) 和0. 0250~0. 3000mg/ml(r=0. 9999) 。本法添加利多卡因1. 0、2. 5,8. 0ug 的回收率分别为101. 51±1. 51%、98. 10±2. 05%和±98. 09±3. 63%,天内变异系数分别为1. 49、1. 29和3. 70,天间变异系数为1. 78%。 相似文献
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高效液相色谱法测定人血液、尿液中的2,4-D丁酯 总被引:1,自引:0,他引:1
目的建立检测血液、尿液中2,4-D丁酯的高效液相色谱分析方法。方法采用正己烷为样品萃取溶剂,色谱柱为Zorbax SB-Aq柱,流动相为V(甲醇)∶V(水)=60∶40。结果 2,4-D丁酯在血液和尿液中的线性范围分别为0.10~10.00μg/mL(r≥0.999 8)和0.08~8.00μg/mL(r≥0.999 5),检测限分别为0.002 0μg/mL和0.001 8μg/mL,准确度为94.5%~104.5%,日内、日间精密度≤4.5%。结论本研究建立的血液、尿液中2,4-D丁酯的提取和HPLC检测方法,可应用于2,4-D丁酯中毒的快速检验和中毒死亡的法医学鉴定。 相似文献
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反相高效液相色谱法测定全血中青霉素类药 总被引:1,自引:0,他引:1
建立了反相高效液相色谱法测定血液中青霉素类药物。0.5ml血液中,加入甲砜霉素作为内标物,经乙腈沉淀蛋白后,上清液直接进样分析。五种青霉素类药物的线性良好,r为0.9918~0.9996;相对回收率为91.75%~107.33%;变异系数为3.56%~9.85%。考察了保存温度对药物分解的影响以及药物在动物血液中消除时限,排除了其它抗生素的干扰 相似文献
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高效液相色谱法检测人脊髓中的利多卡因 总被引:3,自引:1,他引:2
建立高效液相色谱法对人脊髓中利多卡因进行分析的方法 ,以扩大药 (毒 )物检测范围及检测手段 ,适应法医学鉴定及对特殊检材检验的需要。用利多卡因标准品及空白脊髓标准添加实验 ,对色谱条件、样品处理方法、回收率及方法的线性和精密度进行了系统考察。所建方法线性范围是2 0~20 0μg/ml(r=0 9999) ,最低检测限为0 2μg/ml(S/N≥3),加样回收率为82 4 %~92 7% ,该法选择性好、不受干扰。所建方法灵敏、准确、简捷 ,可用于法医学鉴定。 相似文献
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Traditional forensic soil comparisons are performed via physical and/or chemical examinations of color, texture, and mineral content, leaving any organic- or water-soluble fractions unexamined. This study uses high-performance liquid chromatography (HPLC) and ion chromatography (IC) to assess the qualitative and quantitative variation in these fractions of soil. Soil samples (n=120) were collected over the course of 3 weeks from urban, suburban, and rural locations in and around Lansing, MI. Additional samples from six of these locations (two urban, two suburban, and two rural) were collected once a week for 10 weeks for temporal analysis. Nine additional samples, equally spaced over a 1 m(2) grid, from these same six locations were collected for spatial analyses. Qualitative and quantitative analysis of the resultant chromatograms separated the 120 samples into 10 groups by HPLC and 23 groups by IC. This study shows that using HPLC and IC to analyze the organic- and water-soluble fractions of soil can successfully discriminate samples. Quantitative analysis of the results eliminates some false inclusions by providing further differentiation of samples. The results of this study indicate that adding HPLC and IC analyses to traditional forensic soil analysis schemes can improve overall sample differentiation. The methods used in this study were also able to detect both qualitative and quantitative variations in soil over a relatively small geographic area. This demonstration of soil heterogeneity underscores the importance of the collection of a representative known sample population when assessing a forensic soil comparison. Significant temporal variation was also demonstrated over the course of 10 weeks of sampling; however, samples were found to be consistent over shorter periods of time. Baseline levels of inorganic anions were determined via IC; these levels may be useful in assessing the significance of anions detected in soil from cases involving low explosives. 相似文献
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A reversed-HPLC method was established to determinate penicillins in whole blood. Thiamphenicol as internal standard was added to 0.5 ml blood. Proteins in blood were precipitated with acetonitrile, then the separate supernatant was directly injected onto the chromatography column. The range of five penicillins' linearities was 0.9918 to 0.9996, their range of relative recovery was 91.75% to 107.33%, their CV was 3.56% to 9.85%. Effects of different storage temperatures on the stability of analytes and interference of other antibiotics were also studied. 相似文献
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A rapid, sensitive and selective high-performance liquid chromatography tandem mass spectrometric method (HPLC/MS-MS) has been developed and validated for the determination of bromadiolone in whole blood using warfarin as an internal standard (IS). Bromadiolone was extracted from the whole blood samples by liquid-liquid extraction with ethyl acetate. Multiple-reaction monitoring (MRM) was used to detect bromadiolone and IS, using precursor --> product ion combinations at m/z 527 --> 465 and 307 --> 161, respectively. The calibration curve was linear (r2=0.998) in the concentration range of 0.5-100.0 ng/mL with a lower limit of quantification of 0.5 ng/mL in whole blood. Intra- and inter-day relative standard deviations (R.S.D.s) were less than 7.5 and 11.9%, respectively. Recoveries of bromadiolone ranged from 82.1 to 85.2%. This method is found to be determined trace bromadiolone in whole blood and can be used in the diagnosis of the poisoned human beings. 相似文献
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变性高效液相色谱及其在法医DNA分析中的应用 总被引:2,自引:1,他引:1
变性高效液相色谱(DHPLC)是目前检测基因突变的最佳技术平台之一。本文简要介绍了DHPLC的工作原理及主要技术特点,并对其在法医DNA分析中的应用进行综述。 相似文献
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《Forensic science international》1999,102(1):13-22
A supercritical fluid chromatographic method with UV detection (SFC–UV) for the quantitative separation of phenylisothiocyanate (PITC)-derivatised amphetamines is described and compared to high-performance liquid chromatography–diode array detection (HPLC–DAD), gas chromatography–flame ionisation detection (GC–FID) and capillary zone electrophoresis–diode array detection (CZE–DAD) analyses of amphetamine and related compounds. Difficulties in the analysis of common amphetamines by SFC are discussed. Of the methods described in this paper, the SFC method offered the greatest sensitivity at 0.01–0.02 μg of drug on column. Suggestions are made for the use of combinations of these techniques for the identification of amphetamines when gas chromatography–mass spectrometry is not available. 相似文献
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A new solid-phase extraction procedure for the determination of cocaine and some of its metabolites in brain tissue, using high-flow co-polymeric sorbents is reported as a substantial improvement on our recently reported procedure. The recovery of cocaine, norcocaine and cocaethylene was excellent as was the reproducibility of the extraction. The use of high-flow sorbents allowed the easy extraction of tissue without the need for a time-consuming lipase digestion, regardless of sample viscosity or physical nature. The use of these solid-phase columns provided many advantages over the more commonly used solvent extraction, including an increase in extraction speed and efficiency, reduced operator time, reduced solvent use and disposal volumes and exceptional extract quality. The procedure was successfully applied to rabbit brains spiked with cocaine, benzoylecgonine, norcocaine and cocaethylene. 相似文献
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反相高效液相色谱法测定人血浆中的吲哚美辛 总被引:2,自引:0,他引:2
建立血浆中吲哚美辛的高效液相色谱分析方法 ,扩大药 (毒 )物检测范围及手段 ,以适应法医学鉴定的特殊需要。以空白血浆标准添加吲哚美辛对样品处理方法、线性关系、回收率及精度进行考察 ,并以所建方法对健康受试者的血液浓度进行监测。方法的线性范围是 0 1~ 5 0 μg·ml-1,γ =0 9995 ,最小检出浓度为 0 0 2 μg·ml-1(S/N≥ 3)。日内、日间的方法精密度为 ( 1 1± 0 2 ) % (n =4)和 ( 2 7± 0 6 ) % (n =4) ,加样回收率为 97 5 %~10 4 2 %。所建方法准确、便捷、选择性好 ,可用于法医学鉴定及血液浓度监测 相似文献
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A method is described for the identification of five frequently prescribed benzodiazepines (BZD) (clonazepam, diazepam, flunitrazepam, midazolam and oxazepam) in human hair samples by reversed phase HPLC, following on-line simple enrichment and clean-up on a restricted access extraction column. 50mg of powdered hair were incubated (2h at 45 degrees C) after sonication (1h) in 1 ml of the following solution (methanol:ammonia, 97.5/2.5, v/v). The aliquot was centrifuged and the methanolic phase transferred to a conical tube and evaporated under a gentle stream of nitrogen. The residue was reconstituted by adding 100 microl of a mixture of phosphate buffer (20mM, pH=2.2) and acetonitrile (94/6, v/v). A total of 80 microl were injected into the system with the column switching technique. The pre-column or clean-up column was washed with phosphate buffer pH=7.2. The drugs retained on the pre-column were then eluted in the back-flush mode and separated on a C(8) semi micro column, Lichrospher select B, 125 mm x 3 mm. The BZD were determined by a photodiode-array detector at 254 nm, using reference data (retention time and UV spectra) stored in a personal library. The method showed excellent linearity between 0.5 and 20 ng/mg of hair for clonazepam, flunitrazepam and midazolam and between 0.5 and 100 ng/mg of hair for diazepam and oxazepam. Finally, the present method has been applied to a number of forensic cases in our laboratory. 相似文献