A technique to capture, analyze, and quantify anterior teeth rotations for application in court cases involving tooth marks

Anterior teeth within the human dentition have a specific numerical rotation value. Bite marks show an array of angled indentations, abrasions, microlacerations, and contusions. These marks generally represent the incisal surfaces of the suspect's dentition reflecting the rotation values of the teeth in the dental arch. This study described a method for capturing and analyzing anterior dental rotations. The rotations of individual anterior teeth within the study population were categorized as common, uncommon, and very uncommon according to Allen's classification. In the absence of a large number of incisal patterns present in a bite mark, a single but heavily weighted tooth rotation could be of equal discriminatory potential to several common rotation values. No prevalence studies quantifying individual tooth rotations are available. The measurement of each individual tooth rotation together with its individual discrimination potential will enhance the evaluation of the concordant features observed in bite marks.     

Personal Identification by Morphometric Analyses of Intra‐Oral Radiographs of Unrestored Teeth*

Abstract: The aim of this study was to work out a biometric method for personal identification by comparing simulated antemortem and postmortem digital radiographs of unrestored teeth. Intra‐oral radiographs of the inferior right first molar, without restorations, were acquired at two different times in 70 subjects using a standardized technique followed by a morphometric analysis. The program automatically supplied values of the absolute distances, relative distances, shape factors, moments, perimeter values, and the areas of the triangles, which were obtained by joining key reference points. The values for the homologous samples were compared with the heterologous samples. Statistical analysis was then carried out, resulting in a section point value of 0.9992. Higher correlation coefficients indicate positive identification, with less than 2% risk of false positives, and approximately 3% of false negatives. We have also tested the method on dental records from 30 patients in order to demonstrate the specificity and sensitivity of the system.     

The uniqueness of the human anterior dentition: a geometric morphometric analysis

The analysis of bitemarks has a significant bearing on forensic odontology and has attracted an increasingly sophisticated array of techniques in its evaluation. Two postulates underlie all bitemark analyses: firstly, that the characteristics of the anterior teeth involved in the bite are unique, and secondly, that this uniqueness is accurately recorded in the material bitten. Here, we investigate the question of the uniqueness of the anterior dentition. To do this, we use geometric morphometric techniques based on landmark and semilandmark data. The incisor and canine occlusal surfaces of 50 randomly selected orthodontic casts of young individuals (17-20 years) of both sexes form the material for this study. We analyzed the sizes of these teeth by means of landmark and semilandmark analysis to calculate Procrustes distances between tooth outlines. In order to analyze shape variation among individuals, we carried out principal components analyses on the partial warp scores. These are derived from Partial Procrustes coordinates aligned by means of thin-plate spline decomposition based on the bending energy matrix. The results indicate that there is no sexual dimorphism in the shape of the upper or lower arches. Plots of centroid size and first relative warps show less superposition among individuals than in shape analysis. This means that, when the size and shape are considered together, the difference between arches increases. Procrustes superimposition between the two individuals located most closely (0.0444) and the two most separated (0.1567) along the first axis of relative warp analyses show that individuals are not only differentiated by the relative position of their teeth but also by their arch shape. In conclusion, it appears that the incisal surfaces of the anterior dentition are in fact unique.     

Effectiveness of comparison overlays generated with DentalPrint software in bite mark analysis

Validation studies of the new DentalPrint software were carried out with experimental bite marks in pigskin. The bite marks were digitally photographed according to the ABFO guidelines for evidence collection. Dental casts used in the experiment were scanned in 3D and 2D, and comparison overlays were generated using DentalPrint and Adobe Photoshop software, respectively. Digitized photographs of the experimental bite marks and the biting edges obtained in the overlays were compared by two different examiners to analyze the impact of training and experience with the two methods. Receiver operating characteristic (ROC) analysis, sensitivity, specificity, and 95% confidence intervals for each cutoff point were calculated. The expert examiner using DentalPrint obtained the best results, with an area under the ROC curve of 0.76 (SE=0.057; CI at 95%=0.652-0.876). Fairly high specificity values were found for DentalPrint, and the best results were obtained for the cutoff value that discriminated between the examiner's response "biter" and the rest of the possible (specificity 97.9%, CI at 95%=93.2%-99.6%). Therefore, the results presented here indicate that DentalPrint is a useful, accurate tool for forensic purposes, although further research on the comparison process is needed to enhance the validity of bite mark analysis.     

Reconstruction of Deformed Bite Marks Using Affine Transformations

Abstract: Bite marks inflicted on animate and inanimate objects will undergo a certain degree of deformation. This fact remains one of the biggest stumbling blocks when analyzing evidence for court presentation. It has been demonstrated that the reliability of pattern association analysis will not be affected by minimal degrees of warping, shrinkage, and distortion. In this study, affine transformations were applied to bite marks to establish if minimal distortions would affect the mathematically determined relationships of the defined features. In a real case study, it was then tested whether the distorted bite mark matched the dentition of the perpetrator by applying an affine transformation. This was confirmed to be the case. Affine transformations will thus not affect the relationships of the individual features found in bite marks. The numerical calculations validate the reliability of pattern association analysis in the presence of minimal amounts of warping, shrinkage, and distortion.     

Contrast Enhancement of Bite Mark Images Using the Grayscale Mixer in ACR in Photoshop®

Enhanced images may improve bite mark edge definition, assisting forensic analysis. Current contrast enhancement involves color extraction, viewing layered images by channel. A novel technique, producing a single enhanced image using the grayscale mix panel within Adobe Camera Raw^®, has been developed and assessed here, allowing adjustments of multiple color channels simultaneously. Stage 1 measured RGB values in 72 versions of a color chart image; eight sliders in Photoshop^® were adjusted at 25% intervals, all corresponding colors affected. Stage 2 used a bite mark image, and found only red, orange, and yellow sliders had discernable effects. Stage 3 assessed modality preference between color, grayscale, and enhanced images; on average, the 22 survey participants chose the enhanced image as better defined for nine out of 10 bite marks. The study has shown potential benefits for this new technique. However, further research is needed before use in the analysis of bite marks.     

A Morphological and Morphometric Study of Bite Marks Caused by Mice (Mus Musculus) on Different Baits for Forensic Purposes

In animal bites, the dental attributes can be fundamental in identifying the marks made by various species on different matrices. Although rodent bite marks have been studied in the context of postmortem interference, little research has used different baits to analyze these marks linking not only specific behavior patterns but also the possibility of structural damage. Twenty mice (Mus musculus) were exposed to different baits to study their bite marks in a controlled model. The known pattern of parallel and multiple grooves has been seen in all baits, but polyvinyl chloride and fiber‐optic cable were significantly different between each other and the other baits. Some baits showed patterns of anchorage of the upper incisors and space between the lower incisors when gnawing. This technical note represents a novel model of analysis where veterinarians and/or dentists may be asked to give an opinion on alleged animal bite marks.     

Scavenger Species-typical Alteration to Bone: Using Bite Mark Dimensions to Identify Scavengers

Scavenger-induced alteration to bone occurs while scavengers access soft tissue and during the scattering and re-scavenging of skeletal remains. Using bite mark, dimensional data to assist in the more accurate identification of a scavenger can improve interpretations of trauma and enhance search and recovery methods. This study analyzed bite marks produced on both dry and fresh surface deposited remains by wild and captive red fox (Vulpes vulpes) and Eurasian badger (Meles meles), as well as domestic dog (Canis familiaris). The bite marks produced by foxes were distinguishable from those made by badgers and dogs based on ranges of mean length and breadth of pits. The dimensional data of bite marks produced by badgers and dogs were less discernible. Bone modifications vary due to a variety of factors which must be considered, such as scavenger species-typical scavenging behavior, scavenger species' dentition, condition and deposition of remains, and environmental factors.     

A comparison of animal jaws and bite mark patterns

The purpose of this study was to compare the jaw shapes and bite mark patterns of wild and domestic animals to assist investigators in their analysis of animal bite marks. The analyses were made on 12 species in the Order Carnivora housed in the Mammalian Collection at the Field Museum of Natural History in Chicago, Illinois. In addition to metric analysis, one skull from each species was photographed as a representative sample with an ABFO No. 2 scale in place. Bite patterns of the maxillary and mandibular dentition were documented using foamed polystyrene exemplars, which were also photographed. A total of 486 specimens were examined to analyze the jaw and bite mark patterns. A modified technique for measuring intercanine distances was developed to more accurately reflect the characteristics seen in animal bite marks. In it, three separate areas were measured on the canines, rather than just the cusp tip. This was to maximize the amount of information acquired from each skull, specifically to accommodate variances in the depth of bite injuries.     

Dental shape match rates in selected and orthodontically treated populations in New York State: a two-dimensional study

Forensically identifying a suspect's dentition from a bitemark in an open population requires the supposition that every person's dental alignment is different. There have been few studies that have tested this claim. Four hundred and ten lower anterior dentitions from a selected population and 110 lower anterior dentitions from one that was orthodontically treated were measured using geometric morphometric analysis, allowing comparison of arch shape. Dental match rates of 1.46% and 42.7% of individuals were found in the respective populations, given an established measurement error. Orthodontic treatment had a strong effect on match rate suggesting that treated or naturally well-aligned dentitions may be indistinguishable. Sexual dimorphism was found to be only slightly significant. Principal shape variation in both populations was degree of arch curvature. Results of studying these populations show that dental matches can occur, and that statements of certainty concerning individualization in such populations should be approached with caution.     

Yet another method for marking incisal edges of teeth for bitemark analysis

Over the years many methods have been proposed and presented for marking or "highlighting" the incisal edges of anterior teeth on a dental stone model -- one of the critical steps in production of transparent overlays for bitemark analysis. A method is presented here that is an extension and refinement of the "paint the edges" method shown at least as early as 1966 by Gustafson. This method uses "invisible ink" and ultraviolet illumination to produce a very high contrast image of the incisal edges of the teeth on the model. The advantages of this new method are that it is nondestructive, it does not leave visible marks on the models, operator subjectivity in selecting the portions of anterior teeth to be highlighted is reduced, and the author submits that this technique lends itself to easy construction of overlays using computer image-processing software such as Adobe Photoshop and ImageJ.     

咬痕个体识别的比对方法

目的 建立一个牙模数据库,为通过牙齿、咬痕进行个人识别的研究及其在法医实践中的应用提供一种新方法。方法 经过对400例牙模进行分析、计算,将牙齿的形状、排列转换成数字型(X,Y)坐标,通过计算出每颗牙关键点与坐标原点的相对位置,在DOS系统下运用Borland C++5.0语言编写程序,快速地计算出牙齿和牙弓的特征。结果 能对牙模进行自动识别,达到排除与认定。其比对精度长度为1.3mm,角度3°;可取的最少牙点位数目为12～14较为合理,大致需要5～7个完整的牙痕。结论 该方法是研究咬痕与牙模比对的基础。     

Sexual Dimorphism of the First Rib: A Comparative Approach Using Metric and Geometric Morphometric Analyses

This research investigated the sexual dimorphism of the first human rib using geometric morphometric and metric approaches on a sample of 285 specimens containing European Americans and African Americans from the Hamann‐Todd collection. Metric measurements were investigated for sexual dimorphism and ancestral differences using univariate statistics. Four type II landmarks and 40 sliding semi‐landmarks were placed outlining the dorsal and ventral curvatures of the ribs. Landmark data were processed using Generalized Procrustes Analyses with Procrustes distance sliding, and the subsequent coordinates were investigated for sexual dimorphism and ancestral differences using Procrustes ANOVAs. Both geometric morphometric and metric data were analyzed using cross‐validated discriminant function analyses to test the hypothesis that variables from both approaches can be combined to increase sex classification rate. European Americans had sex correctly classified as high as 88.05% and African Americans as high as 70.86% using a combination of metric and geometric morphometric variables.     

Patterns of Variation and Match Rates of the Anterior Biting Dentition: Characteristics of a Database of 3D‐Scanned Dentitions

An understanding of the variability of the anterior human dentition is essential in bitemark analysis. A collection of 1099 3D laser scans of paired maxillary and mandibular arches were studied using geometric morphometric methods. Analyses were performed without scale (shape only) and with scale (shape and size). Specimens differing by no more than experimentally obtained measurement error were counted as matches, or as indistinguishable. A total of 487 maxillary (396 size preserved), 131 mandibular (83 size preserved), and one paired dentition (two size preserved) matches were found. Principal component analysis and partial least squares revealed interpretable patterns of variation and covariation in dental shape, principally dominated by variation in dental arch width. The sensitivity of match rate to assumed degree of measurement error was also determined showing rapid increases in match rate as measurement error increased. In conclusion, the concept of dental uniqueness with regard to bitemark analysis should be approached with caution.     

Bite Mark Analysis in Foodstuffs and Inanimate Objects and the Underlying Proofs for Validity and Judicial Acceptance

Even though one of the first bite mark cases was Doyle v. State in 1954 (a bitten cheese case), the research has focused on bite marks inflicted in human skin. As published Papers, Case Reports, or Technical Notes can constitute precedents which are relied upon in making the legal arguments and a considerable amount of case law exists in this area, we present a systematic review on bite mark analysis in foodstuffs and inanimate objects and their underlying proofs for validity and judicial acceptance according to Daubert rulings. Results showed that there is vulnerability in these procedures, and it is essential to demand for focus scrutiny on the known error rates when such evidence is presented in trials. These kinds of bite marks are well documented; however, there has been little research in this field knowing that the protocols of analysis and comparison are the responsibility of the forensic odontologists.     

A suggested classification of bite marks in foodstuffs in forensic dental analysis

Bite marks have been reported in flesh, foodstuffs and inanimate objects. Those in foodstuffs occur widely in cases of larceny but also occur in serious crimes such as murder.Evaluation of distinctive characteristics in food bites differs from the corresponding assessment of flesh bite marks in that the assessment is made on the impression made by the labial aspect of the teeth and not on the biting edge, because the teeth penetrate the bitten foods to different depths.The terminology used to describe food bite marks is very varied and a classification of food bites has been formulated in an effort to bring a degree of uniformity to the analysis of such marks.     

Quantification of Osteon Morphology Using Geometric Histomorphometrics

Many histological methods in forensic anthropology utilize combinations of traditional histomorphometric parameters which may not accurately describe the morphology of microstructural features. Here, we report the novel application of a geometric morphometric method suitable when considering structures without anatomically homologous landmarks for the quantification of complete secondary osteon size and morphology. The method is tested for its suitability in the measurement of intact secondary osteons using osteons digitized from transverse femoral diaphyseal sections prepared from two human individuals. The results of methodological testing demonstrate the efficacy of the technique when applied to intact secondary osteons. In providing accurate characterization of micromorphology within the robust mathematical framework of geometric morphometrics, this method may surpass traditional histomorphometric variables currently employed in forensic research and practice. A preliminary study of the intersectional histomorphometric variation within the femoral diaphysis is made using this geometric histomorphometric method to demonstrate its potential.     

Quantification of Maxillary Dental Arcade Curvature and the Estimation of Biological Ancestry in Forensic Anthropology

Previous studies suggest that palate shape is a useful indicator of biological ancestry in human remains. This study evaluates interobserver error in ancestry estimation using palate shape and explores palate shape variation in Gullah (descendants of West Africans) and Seminole (Indigenous American) population samples using geometric morphometric analysis. Ten participants were asked to ascribe biological ancestry and shape to 28 dental casts based on a classification scheme employed in previous studies. The mean correct classification was 42.0%, indicating that the likelihood of assigning the correct ancestry is very poor and not significantly different from random assignment (p = 0.12). The accuracy analysis based on categorical classification of the casts was complemented by geometric morphometric analysis of nine 3D landmarks reflecting palate shape of 158 casts. Principal component analysis results show no difference between populations regarding palate shape, and cross‐validated discriminant function analysis correctly classified only 62.0% of the specimens. Combined, these results show that previous methods to estimate ancestry are inaccurate and that this inaccuracy is probably due to a lack of palate shape differences between groups, rather than limitation of the analytical method per se. Therefore, we recommend caution should be used when choosing to apply the analysis of palate shape in forensically relevant contexts.     

In the eye of the beholder: sex and race estimation using the human orbital aperture

From the works of Broca and Krogman to modern-day Jantz and Buikstra, the orbit has been used for both quantitative and qualitative sex and race estimation. This study evaluates the practical value of these estimations. Orbital height and breadth were measured to determine the orbital index and assess differences between men and women or black people and white people in the Hamann-Todd Collection. Replicability of these measures was also examined. Finally, a geometric morphometric study was performed to assess shape differences using the entire margin. Significant differences were found in both the index and the geomorphometric study; however, further investigation revealed that the differences were of little practical use. The measurement differences were found to be smaller than intra-observer error, while the geometric morphometric analysis demonstrated that minimal percentage of variation in shape was attributable to group differences. Thus, these techniques should not be used to estimate sex or race.     

An integrated technique for the analysis of skin bite marks

The high number of murder, rape, and child abuse cases in South Africa has led to increased numbers of bite mark cases being heard in high courts. Objective analysis to match perpetrators to bite marks at crime scenes must be able to withstand vigorous cross-examination to be of value in conviction of perpetrators. An analysis technique is described in four stages, namely determination of the mark to be a human bite mark, pattern association analysis, metric analysis and comparison with the population data, and illustrated by a real case study. New and accepted techniques are combined to determine the likelihood ratio of guilt expressed as one of a range of conclusions described in the paper. Each stage of the analysis adds to the confirmation (or rejection) of concordance between the dental features present on the victim and the dentition of the suspect. The results illustrate identification to a high degree of certainty.