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1.
本文将双波长法用于二阶导数光谱中,提出一种新的COHb饱和度测定方法。用本法测定了已知COHb饱和度的标准血样和未知COHb饱和度的检血,都得到了比较满意的结果。  相似文献   

2.
通过分析近年来有关血液样品中碳氧血红蛋白稳定性方面的研究资料,发现血液中碳氧血红蛋白的稳定性受到盛放容器、保存温度、容器顶部空气体积、初始HbCO饱和度及防腐剂的添加与否的影响,其中保存温度、容器顶部空气体积及初始HbCO饱和度则是其主要影响因素。  相似文献   

3.
95例尸体血中HbCO%的分析   总被引:4,自引:2,他引:4  
血中碳氧血红蛋白饱和度(简称HbCO%,下同)含量是判断一氧化碳中毒,推断火灾中尸体生前状态的依据。本文证明:血中HbCO%与其年龄、性别及不同情况下CO中毒有关。一氧化碳中毒的尸体含量较高(平均大于60%);火灾事故中遇难的尸体含量中等(平均在30~50%);而被杀后投入火场或服毒后自焚则较低(平均小于20%)。  相似文献   

4.
四阶导数光谱法测定血液碳氧血红蛋白饱和度   总被引:1,自引:0,他引:1  
本文报导了两种利用四阶导数光谱测定血液 HbCO%的方法。一种需从检血制备一氧化碳饱和血溶液,操作稍繁但准确可靠,受仪器稳定性影响较小。另一种不需制备一氧化碳饱和血溶液,是一种操作简单,快速亦准确可靠的方法,但由于计算中使用预先测定的常数,测定的准确性乃受仪器稳定性波动的影响。  相似文献   

5.
检验血液中碳氧血红蛋白饱和度越来越多地应用仪器进行定性、定量分析,其中紫外光谱法由于简单、方便,应用较普遍。但该法需要对所得到的紫外光谱图进行手工测量计算,常常产生较大的误差。本文采用Matlab软件,根据碳氧血红蛋白饱和度的计算方法编写了一个程序,消除了这种人为的误差,并实现了测量过程的自动化,且计算快速、简便。Mat-lab软件计算功能强大,提供了一种演算纸方式的编程语言,已成为科学研究和工程计算的基本技能[1,2],但在法庭物证科学中的应用还很少,现报道如下。1一氧化碳中毒血紫外吸收光谱图取0.2ml检血,加适量的蒸馏水稀…  相似文献   

6.
<正> 导数光谱法用于血液碳氧血红蛋白饱和度的测定是从70年代未见于文献的。不仅能克服普通光谱法的缺点,而且还具有更高的灵敏度、准确性和重现性。已报导的导数光谱法有二阶导数光谱法和四阶导数光谱法,都是首先将检血还原成还原血红蛋白(Hb)和碳氧血红蛋白(H  相似文献   

7.
血液样品中防腐剂对碳氧血红蛋白稳定性的影响   总被引:1,自引:0,他引:1  
目的研究临床上常用8种试剂对血液样品中碳氧血红蛋白(HbCO)稳定性的影响。方法将血液样品分为高、低两个HbCO浓度组,选用临床常用的甲醛、氟化钠、乙二胺四乙酸二钠、亚硝酸钠、草酸钾、肝素钠、柠檬酸钾及氟化钠与草酸钾混合物(1:3)8种试剂,按常用浓度分别添加到血样品中,并于添加后0h、2h、8h、24h、3d、7d用紫外可见光分光光度法检测其中HbCO饱和度,用统计学方法进行结果分析。结果本实验选用的8种试剂只有甲醛和亚硝酸钠对HbCO的稳定性影响较为显著,而其余6种对HbCO稳定性的影响无统计学意义。结论在检验疑似CO中毒并经甲醛或亚硝酸钠防腐的检材时应慎重,以免导致错误的鉴定结论。  相似文献   

8.
不同保存条件对血中CN-浓度的影响   总被引:2,自引:0,他引:2  
目的研究在各种温度贮存条件下血液中CN-浓度与保存时间的关系。方法用氯胺T衍生GC/ECD分析。结果在室温下存放的阳性血,在30天~50天中,CN-浓度有所增加,以后浓度下降很快,80天后则检不出。在4℃及-20℃下贮存的血液CN-浓度,在一定的时间段中,有所增加,以后则逐渐下降,至8个月仍可检出CN-。结论不同条件下存放的血液CN-其浓度的变化是不同的。  相似文献   

9.
血和肝脏检材保存条件对氰化物浓度的影响   总被引:1,自引:0,他引:1  
研究了大白鼠血液和肝脏检材保存时间及保存温度对氰化物浓度的影响。研究表明,保存168h,保存温度4℃,血液和肝脏检材中的氰化物含量变化很小;保存温度25℃,血液和肝脏险材中的氰化物浓度随时间延长而上升,肝脏检材中氰化物浓度在168h为4h的1.17倍。4h染毒组大白鼠氰化物浓度血中比肝脏中高2.3倍,证实了氰化物在体内分布的差异性。  相似文献   

10.
苯巴比妥在家兔血保存标本中分解动力学研究   总被引:1,自引:0,他引:1  
本实验研究了家兔尸体血液室温保存组(10~15℃)和冰箱保存组(4℃)中苯巴比妥的分解动力学。结果表明,苯巴比妥在血液保存标本中分解是非常缓慢的,室温保存组和冰箱保存组的分解速度相近。此结果为疑为苯巴比妥中毒案件中正确评价毒物学分析结果提供了一定的参考依据。  相似文献   

11.
人体内乙醇含量检测的影响因素分析   总被引:2,自引:2,他引:0  
人体内乙醇含量检测不仅是法医鉴定工作中常规检测项目,也是交通肇事案件最终的仲裁依据,其检测结果直接影响着受检人员的责任判罚。本文根据乙醇的毒理特征及在人体内的代谢过程,就不同检材、送检时效性、尸体腐败、血液检品中不同成分和保存方式、以及检测结果的不确定度等对乙醇含量检测结果的影响进行分析,以利于对受检者是否饮酒及其程度做出科学、公正的判定。  相似文献   

12.
The spectrophotometric method for the determination of carboxyhemoglobin (HbCO) in blood reported by Fretwurst and Meinecke was modified so as to give the same values of percentage HbCO (HbCO%) as those determined by the oxygen electrode method. Values of HbCO% of nine practical samples determined by both the oxygen electrode method and the present method were nearly identical regardless of the presence of methemoglobin (Met-Hb) in blood. The present method is suitable for forensic practice.  相似文献   

13.
This paper describes the spectrophotometric determination of carboxyhemoglobin (CO-Hb) in blood on the basis of double wavelength spectrophotometry of absorbance difference. Absorbance measurements are made in the 500–600 nm region at a blood dilution of 100–200-fold. Blood is diluted with a solution containing Na2S2O4 to provide two components of CO-Hb and deoxyhemoglobin (deoxy-Hb). Absorbance difference at the two wavelengths at which deoxy-Hb has the same absorbance reflects only the CO-Hb component because the opposite component is nulled out of the mixture. After measurement of the absorbance difference, the measuring solution is saturated with CO gas to make all Hb derivative CO-Hb and remeasured at the same wavelengths. The percent of CO-Hb is considered the absorbance difference ratio. Results obtained by the present method was in satisfactory agreement with gas chromatographic data in blood not containing methemoglobin (Met-Hb). Comparative experiments using the gas chromatographic method and the present method were performed with samples containing Met-Hb. However, while there is a deficiency in the gas chromatographic method when the samples contain Met-Hb, the results of the present method were in close agreement with theoretical values when samples are mixed with CO-Hb, O2-Hb and Met-Hb. Advantages of this method are that it is simple and accurate, standard curve or equation for calculation and accurate dilution are not necessary.  相似文献   

14.
Kinetics of the oxidation of carboxyhemoglobin (HbCO) by potassium ferricyanide was studied photometrically in a weakly acid solution. An increase in the absorbance at 630 nm reached a maximum within 10 min when over a 100-fold excess of ferricyanide to hemoglobin iron was used. A slight decrease in the absorbance was observed after completion of the reaction when over a 500-fold excess of the reagent was used. In the presence of 0.4% Sterox SE, the absorbance began to decrease without complete oxidation. From these findings, a simple, rapid and accurate method for the determination of methemoglobin (Met-Hb) in blood was devised. The method was compared with two other methods, using 11 blood samples containing various amounts of HbCO, and proved to be suitable for blood containing elevated HbCO as well as for ordinary blood.  相似文献   

15.
Lubricating oil comparisons impact a variety of forensic investigations, including cases where oil was transferred from a suspect vehicle to the crime scene or victim. In this study, high-temperature gas chromatography/mass spectrometry was used to examine the influence of oil mixtures and oil changes over time on the comparison of known and questioned lubricating oils. Varying concentrations of oil mixtures were prepared and showed the potential for identifying individual components. Motor oils from 18 automobiles monitored over a 2-month period did not demonstrate significant changes in the chromatographic data. Chemometric analysis of motor oil mass spectral data provided little information regarding differentiation of, or changes in, the samples. Power steering fluid (PSF) from a naturally occurring leak collected from several locations was consistent with the PSF in the automobile's reservoir, and the PSF composition did not change over time.  相似文献   

16.
Chu JX  Shen JH  Jiang WH 《法医学杂志》2006,22(3):217-219
目的探索乙酰甲胺磷中毒在血液灌流(HP)过程中血药浓度的变化规律及终末浓度。方法采用医院急诊病例病人股静脉血,通过气相色谱法分析血浆中的乙酰甲胺磷含量。结果经血液灌流后血浆中乙酰甲胺磷浓度迅速下降,并呈现出灌流前血药浓度越高下降速度越快的特性。结论血液灌流法能迅速降低血液中乙酰甲胺磷浓度,其下降的速度受制于乙酰甲胺磷的初始浓度和灌流开始时间等。  相似文献   

17.
目的探讨硫化氢(H2S)中毒者血中H2S的检测及特征鉴别;方法取新鲜空白血液和5℃下保存1月、1年的血液各2m L,经微波低火加热1min后,吸取顶空气体100μL进行GC/PFPD-S、GC/MS检测;取相同样本进行酸化处理后进行检测;取疑似为H2S中毒者血液直接加热后进行检测。结果新鲜血液和5℃下保存1月、1年的血液均可检出H2S成分,但保存1个月以上的血液可同时检出二硫化碳(CS2)成分;酸化处理后的血液,H2S和CS2成分检出的含量明显比未加酸化的血液高;中毒者血液中除了检出H2S成分外,还检出甲硫醚成分。结论血液样本同时检出H2S和较大量的CS2时,不宜判定为H2S中毒,提示可能为腐败所致,而同时检出H2S和较大量的甲硫醚,则可做出H2S中毒的判断。  相似文献   

18.
Yan YY  Liao LC  Yang L 《法医学杂志》2004,20(1):23-25
目的探讨甲醛对一氧化碳中毒血的检测是否产生干扰,提高一氧化碳中毒鉴定的可靠性。方法采用常用的血中一氧化碳或碳氧血红蛋白饱和度的检测方法对未加甲醛和加甲醛的血样分别进行实验研究。结果甲醛对加热法、氢氧化钠法、氯化钯法、分光光度法等检测方法均可产生不同程度的干扰。结论经福尔马林灌注或固定的检材不宜用于一氧化碳中毒血检测,否则可能导致错误的鉴定结论。  相似文献   

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