Proteinase K challenged by a novel protease

Proteinase K is used in forensic DNA extraction methods for cell lysis and degradation of proteins. Here we compare Proteinase K with a novel protease. We conclude that there is no need to exchange Proteinase K in our methods.     

Molecular study of time dependent changes in DNA stability in soil buried skeletal residues

In the past years, many publications about identification and sex-determination of dry human bones by means of DNA analysis have been published. However, few studies exist that investigate the potential use of DNA technique to determine the postmortem interval (PMI). In the present study we analyzed the rate of increasingly smaller fragments of chromosomal DNA and PMI.     

Can Situational and Structural Factors Differentiate Between Intimate Partner and “Other” Homicide?

A large body of research has identified strong and consistent correlates of aggregated intimate homicide incidents; however, the bulk of these studies focuses on the influence of either case or neighborhood characteristics on homicide types, but not both. This study examines data collected from 739 homicides in two distinct metropolitan cities to determine which factors differentiate intimate and non-intimate homicide. Findings reveal that intimate partner homicides (IPH) are more likely to involve females both as victims and offenders when compared to non-IPH incidents. In addition, IPH homicides are more likely to be committed with weapons than non-IPH, but this finding only appears in Indianapolis homicides. Indeed, one of the stark contrasts between Indianapolis and Newark was the much greater prevalence of firearms involved in IPH homicides in Indianapolis. This suggests the need for future research that sorts out patterns of household gun possession, factors influencing community levels of household gun possession, and the use of legally- or illegally-possessed firearms in IPH homicide.     

Community-level HIV prevention for persons with severe mental illness living in supportive housing programs: a pilot intervention study

Individuals with severe mental illness (SMI) are at risk for HIV/AIDS. Despite the availability of supportive community programs for those with SMI, there have been no published evaluations of community-level HIV prevention trials among this population. A pilot intervention trial was conducted to determine the feasibility of such an intervention in supportive housing programs (SHPs). A multi-component community-level trial was implemented in two SHPs with a total of 28 residents. Participants completed assessments at three time points: prior to the intervention (baseline), following skills training (post-assessment), and following the 4-month community intervention (follow- up). Results demonstrated significant improvements in psychosocial risk factors at both post- and follow-up assessments, with indications of sexual behavior change at follow-up. The community-level intervention appeared to reduce the risk of HIV among persons with SMI living in SHPs, and supports the importance of conducting larger scale intervention trials.     

The problem of single parent/child paternity analysis--practical results involving 336 children and 348 unrelated men

In a certain amount of paternity investigations, only DNA from child and alleged father is analyzed, thus increasing the possibility of false paternity inclusions. The aim of this study was to determine how many wrong paternity inclusions could be detected in a rather small geographical area comparing empirical results from 336 children and 348 men (13-15 STRs were investigated per person). This comparison between each child and all unrelated men (i.e. all putative fathers from the other cases) with an especially designed computer program resulted in 116,004 man/child pairs. Less than three excluding STRs were found in 1666 child/unrelated man pairs (1.44% of the comparisons). At least one unrelated man with only two or less STR mismatches could be determined for 322 children (95.8% of all investigated children). In 26 comparisons no STR mismatches between a child and an unrelated man were detected, thus at least one and up to three "second father(s)" under 350 men could be found for 23 children, if the mother is excluded. Paternity probabilities between 95.475% and 99.996% were calculated. Our results underline the difficulties in motherless paternity cases using only STR analysis and advise great precaution in assigning verbal predicates such as "paternity proven" in those investigations.     

Testing the Trauma Model of Violence

The present study sought to explore the relationship between trauma and violence, as measured by the Traumatic Events Questionnaire and the Conflict Tactics Scale. Using Multiple Regression Analysis, several types of traumatic experiences were studied as predictors to violent behavior in 55 graduate students. Results suggest that trauma as a set, as well as being the victim of a violent crime, are significantly related to future violent behavior. However, no other individual predictor variable, including being the victim of childhood physical abuse, significantly predicted future violence. Implications of the findings are discussed.