STR Profiles from DNA Samples with "Undetected" or Low Quantifiler™ Results

Abstract:  Screening methods capable of identifying DNA samples that will not yield short tandem repeat (STR) profiles are desired. In the past, quantitation methods have not been sensitive enough for this purpose. In this study, low level DNA samples were used to assess whether Quantifiler™ has a minimum quantitation value below which STR profiles would consistently fail to be detected. Buccal swabs were obtained and the DNA extracted, quantified, and serially diluted to concentrations ranging from 0.002 to 0.250 ng/μL. Samples were analyzed once with Quantifiler™, followed by Profiler Plus™ amplification and capillary electrophoresis analysis. An absolute minimum value below which STR results were unobtainable could not be defined. From the 96 low level samples tested, STR loci (including one full profile) were successfully amplified and detected from 27% of the samples "undetected" by Quantifiler™. However, no STR alleles were detected in 73% of these "undetected" samples, indicating that Quantifiler™ data may be useful for predicting STR typing success.     

Anion Identification via Complexation with meso‐octamethylcalix(4)pyrrole and Detection Using Electrospray Ionization Mass Spectrometry*

Abstract: The routine identification of controlled substances and adulterants during forensic chemistry analysis often involves the identification of counter ions or salt forms present in an exhibit. Here, the use of the compound meso‐octamethylcalix(4)pyrrole (C4P) during salt‐form identification analysis is presented. C4P is a commercially‐available, anion‐binding agent that can be reacted with a controlled substance or adulterant, resulting in the sequestration of anionic species, usually present as counter ions to the active ingredient. Formation of noncovalent complexes between the cyclic host C4P compound and anionic guests is investigated using electrospray ionization–mass spectrometry (ESI–MS). Complexes with chloride, bromide, iodide, nitrate, and acetate are readily observed and mass spectrometry analysis provides identification via molecular weight characterization. Chloride and bromide complexes are also characterized by the isotopic distribution of their molecular ions. Formation of host–guest complexes is not observed for sulfate and phosphate salts, presumably due to steric hindrance and energetically unfavorable conditions.     

Whitewashing: How Obama Used Implicit Racial Cues as a Defense Against Political Rumors

Political Behavior - Although Barack Obama won the presidency in 2008, some Whites nevertheless penalized him because of his race. In part, these penalties involved persistent rumors about his...     

Healthcare,Policy Implementation,and Culture: What Cultural Values Influence Unpaid Primary Caregivers to Provide Care to Older Adults?

This research examines home healthcare programs implemented in Puerto Rico and Indiana. Puerto Rico is primarily a Hispanic territory of the United States, while Indiana is a culturally Anglo Midwestern state. This study explores the cultural values that influence unpaid care of older adults in these two settings. I hypothesize that dissimilar cultural values will influence care of unpaid primary caregivers (UPCs) in both jurisdictions. Results supported the hypothesis: UPCs emphasized different cultural values in caregiving. The study recommends that the federal government support families with essential services to enable them render badly-needed care to their elderly family members.     

Mediated territoriality: rural workers and the efforts to scale out agroecology in Nicaragua

The Spanish word formación can be translated as ‘training’ or ‘education’, but Latin American social movements use it as inspired by Che Guevara’s notion of ‘molding’ the values of the new woman and new man for egalitarian, cooperative social relations in the construction of a ‘new society’. This contribution presents findings on the dialectical linkages between the formación processes led by the Rural Workers’ Association (ATC) and the gradual transformation of the Nicaraguan countryside by peasant families choosing to grow food using agroecological practices. We use Vygotsky’s sociocultural historical theory to explore the developmental processes of formación subjects and the pedagogical mediators of their transformation into movement cadre. The motivations of active learners to develop new senses and collective understandings about their material reality become a counterhegemonic process of internalization and socialization of agroecological knowledges and senses. In this paper, we further explore the formación process by identifying territorial mediators: culturally significant elements within and outside of individuals that facilitate the rooting of agroecological social processes in a given territory where the social movement is active. By placing the territory, rather than the individual, at the center of popular education processes, new synergies are emerging in the construction of socially mobilizing methods for producing and spreading agroecological knowledge.