Dominance of pre-analytical over analytical variation for measurement of methadone and its main metabolite in postmortem femoral blood

On the basis of simultaneously sampled postmortem blood specimens from the left and right femoral veins the pre-analytical variation of methadone measurements was evaluated and compared to the analytical variation. The material consisted of a series of 27 duplicate samples from routine autopsy cases comprising mainly drug addicts. A chiral LC-MS/MS method was used for measurement of the R- and S-enantiomers of methadone and its main metabolite 2-ethyl-1,5-dimethyl-3,3-diphenylpyrrolinium (EDDP). The analytical CV% was determined to be in the range 3-4% for methadone enantiomers and 4-6% for EDDP enantiomers. The total measurement uncertainty (CV(T)) was estimated from the pre-analytical variation (CV(PA)), analytical variation proper (CV(A)), and variation related to calibration (traceability) (CV(Cal)) according to the relationship CV(T) = [CV(2)(PA) + CV(2)(A) + CV(2)(cal)](0.5). Uncertainty related to calibration concerned a component related to the purity of drug reference compound and a contribution from the production of calibrator solutions (CV(Cal)<1%). Pre-analytical sampling variation was estimated from the duplicate measurements of blood samples after subtraction of the analytical component. The pre-analytical variation amounted to a CV% of 19-21% for R- and S-methadone and 30-38% for R- and S-EDDP, i.e. considerably larger than the other components. Due to the squared addition principle, the resulting total uncertainty (CV(T)) became largely identical to the CV(PA), i.e. 19-21% for R- and S-methadone and 31-38% for R- and S-EDDP enantiomers. Accordingly, CV(T) exceeded CV(A) by a factor 5 or more. Dominance of the pre-analytical component of variation may also be likely for other compounds measured in postmortem blood samples. Thus, the width of the 95%-uncertainty interval (+/-2CV(T)) for a postmortem measurement is largely determined by the pre-analytical component of variation. This should be kept in mind when judging on the uncertainty of postmortem measurement results.     

Magnetic law: Designing environmental enforcement laws to encourage us to go further

The European Union has some of the world's most ambitious and highly developed environmental laws on its books, but their effectiveness is severely compromised by non-compliance. With the UNECE Aarhus Convention (1998), Europe launched an innovative legal experiment, democratizing environmental enforcement by conferring third party citizens and environmental non-governmental organizations (ENGOs) with legal rights of access to environmental information, public participation, and access to justice in environmental matters. Based on some 2000 surveys and over 150 interviews with stakeholders from three Member States – France, Ireland, and the Netherlands – we adopt a holistic, 360° perspective, capturing the views of regulated parties, NGOs, and the general public on this private governance experiment. Our data provide important new insights into the practical effectiveness of Europe's laws enabling private environmental enforcement, its (intended and unintended) effects on farmers' compliance decisions in the vital area of nature conservation, and how law might be used to stimulate pro-environmental predispositions.     

Results of the 2018 Rapid DNA Maturity Assessment

Three commercially available integrated rapid DNA instruments were tested as a part of a rapid DNA maturity assessment in July of 2018. The assessment was conducted with sets of blinded single-source reference samples provided to participants for testing on the individual rapid platforms within their laboratories. The data were returned to the National Institute of Standards and Technology (NIST) for review and analysis. Both FBI-defined automated review (Rapid DNA Analysis) and manual review (Modified Rapid DNA Analysis) of the datasets were conducted to assess the success of genotyping the 20 Combined DNA Index System (CODIS) core STR loci and full profiles generated by the instruments. Genotype results from the multiple platforms, participating laboratories, and STR typing chemistries were combined into a single analysis. The Rapid DNA Analysis resulted in a success rate of 80% for full profiles (85% for the 20 CODIS core loci) with automated analysis. Modified Rapid DNA Analysis resulted in a success rate of 90% for both the CODIS 20 core loci and full profiles (all attempted loci per chemistry). An analysis of the peak height ratios demonstrated that 95% of all heterozygous alleles were above 59% heterozygote balance. For base-pair sizing precision, the precision was below the standard 0.5 bp deviation for both the ANDE 6C System and the RapidHIT 200.     

The changing face of organized crime in New Jersey

Trends in Organized Crime -     

An honest human body : Sexuality and the continuum of resistance

Theories of resistance have informed ethnographic research over the last two decades. Researchers have been concerned to document the significance of counter-cultural acts of resistance and have highlighted intersections of class, gender and “race” within the processes of resistance. However, questions of sexuality have been largely silenced. This paper focuses on the experiences of six women who identify themselves as lesbian and attempts to assert the importance of sexuality within debates on resistance and accommodation. The experiences of these women reveal that lesbians are willing and able to use a conscious collage of bodily signifiers to resist the discourse of compulsory (hetero)sexuality. The “continuum of resistance” illuminated in this paper can encompass both the lesbian who wears her sexuality on a t-shirt and the lesbian who “passes” in a heterosexual frock.     

Insurance Rationing versus Public Political Rationing: The Case of the Oregon Health Plan

This article describes the development and evolution of the Oregon experiment with public political rationing in health care. As dissatisfaction with current health care rationing has mounted, the search has accelerated for alternatives to replace the de facto rationing generated by the private health insurance marketplace. As the foremost example of public rationing, the closely-watched Oregon Health Plan is widely offered as an alternative to traditional private health insurance. Other health care rationing alternatives include health maintenance organizations (HMOs) and proposals that encourage self-rationing by individual consumers.     

The new chain gang: Corrections in the next century

This article examines the operations and excesses of the correctional chain gang as a group of prisoners working outside a penal facility under armed correctional supervision and wearing chains or other restraining devices to prevent escape. We detail the operations and excesses of earlier chain gangs, identifying variables that contributed to the contemporary phase that emerged in 1995. Predicated on vengeance, retributive justice, and punitiveness, the chain gang represents yet another devolving standard of human decency in correctional policies. Future scenarios are explored.     

The reasons for choosing a career in policing among South Korean police cadets

Despite the importance of understanding the reasons for choosing the police as an occupation for developing effective recruiting strategies, there is a surprising lack of research about what motivates people to choose careers in policing in South Korea. Employing the sample of 410 Korean police cadets, the study found that a financial factor — “steady salary” and “job security” — was the most important factor affecting the decisions of police cadets to join the police. Regression analyses indicated that gender and social-economic status had significant effects on cadets' motivation of joining the police. In the conclusion, policy implications for developing an effective recruitment strategy are discussed.