New alleles and mutational events at 14 STR loci from different German populations

The molecular origin of DNA mutations and the mutation rates were analyzed at 14 short tandem repeat (STR) loci with samples from trio cases derived from 10 different German population samples. STR loci comprised of D2S1360, D3S1744, D4S2366, D5S2500, D6S474, D7S1517, D8S1132, D10S2325, D12S391, D18S51, D19S246, D20S480, D21S226, and D22S689. In a total of 488 meioses, 16 isolated genetic inconsistencies in 8 different STRs were observed, whereas no mutations were found at the other loci. The data of five mutations suggested the presence of silent or null alleles due to sequence variation in primer binding site. This could be confirmed for four suspected cases by the use of alternative primer sets and by DNA sequence analyses. Furthermore, this study revealed nine new allelic variants at five different loci.     

幸运之轮转向中国

中国已成为金融危机和经济危机后最大的赢家。任何到访亚洲的人都会认识到,西方在金融和经济能力方面的声望严重受损,中国却名声大振。幸运之轮正在转向。由此立刻出现了三个问题:     

Deleuze: “Had I not done philosophy I would have done law”

For those too few of us who carry a flame for an idea or shape of community and if necessary law to come Deleuze provides us with some useful signposts and overall a working toolbox with which to tackle both the micro and macro of our daily encounters. The whole package of Deleuzian machines and assemblages can be taken up as an abstract discourse which too outsiders might appear devoid of any linkage to reality. The whole notion of Deleuzian lawyers is one that can easily raise a chuckle, but nevertheless some of us seem to persevere with playing with the law with this toolbox. Martin Hardie, martin.hardie@gmail.com, has managed bands and worked in Aboriginal Art and Craft centres. He has been a solicitor, and a barrister, acting in matters concerning administrative law and constitutional law, and particularly, as counsel for Australian Indigenous artists in their quest for copyright protection, the challenge by the current East Timorese Prime Minister to Australian legislation implementing an agreement with the Republic of Indonesia concerning the division of East Timor’s oil and gas resources, and, in a case concerning legislation in Australia legalising euthanasia. He has also been an advisor to various members of the former East Timorese resistance and government, a university lecturer, a cyclist, cycling journalist and team manager. He currently lives in Bilbao working as an English teacher and writer. His time is spent in the Basque Country with the ambition of becoming the archetype of life within communism; at the break of dawn a cyclist, during the day a teacher and a cook, cyber-conspiracist and correspondent, in the afternoons a student and philosopher and, at nights, simply pleasant company. His home at http://auskadi.tk/     

Post-mortem toxico-kinetics of trazodone.

Trazodone is a structurally unique bicyclic anti-depressant, said to be significantly less toxic than other anti-depressants following an acute overdose. We studied the tissue distribution and post-mortem redistribution of trazodone in two fatalities, one of which allowed comparison with trimipramine, a typical tricyclic anti-depressant. Case 1, a 53-year-old female weighing 72 kg, had femoral vein concentrations of trimipramine 5.5 micrograms/ml, trazodone 14.4 micrograms/ml and alcohol 107 mg%. Case 2, a 48-year-old female of 70 kg, had a femoral vein trazodone of 15.5 micrograms/ml and alcohol 34 mg%, with no other drugs detected. For case 1 and case 2 respectively, trazodone tissue concentrations were: skeletal muscle 7.3 and 9.0 micrograms/g; left and right lungs 13.3, 12.9 and 35.3, 40.1; myocardium, 30.9 and 28.9; kidneys 34.7 and 39.6; liver 73.7 and 82.4; fat 18.5 and 16.5; brain 48.6 and 20.9. For case 1 and 2, respectively, blood trazodone concentrations in 10 initial autopsy samples ranged from 13.7-17.3 and 14.4-16.9 micrograms/ml. Twenty-four and forty-eight hours later the respective ranges were 12.8-18.0 and 12.4-19.9 for case 1, 12.5-20.1 and 12.7-27.0 for case 2. By contrast, for trimipramine, blood concentrations at 0 time, 24 h and 48 hours ranged from 5.5-11.4, 5.2-14.3, and 4.2-18.2, respectively. We conclude that trazodone shows little preferential concentration in solid organs and consequently has relatively stable post-mortem blood concentrations with little drug redistribution artefact. Both the clinical pharmacokinetics and post-mortem toxicokinetics of trazodone differ significantly from the tricyclic anti-depressants.