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Peng Liu Stephanie H.I. Yeung Karin A. Crenshaw Cecelia A. Crouse James R. Scherer Richard A. Mathies 《Forensic Science International: Genetics Supplement Series》2008,2(4):301-309
An integrated lab-on-a-chip system has been developed and successfully utilized for real-time forensic short tandem repeat (STR) analysis. The microdevice comprises a 160-nL polymerase chain reaction reactor with an on-chip heater and a temperature sensor for thermal cycling, microvalves for fluidic manipulation, a co-injector for sizing standard injection, and a 7-cm-long separation channel for capillary electrophoretic analysis. A 9-plex autosomal STR typing system consisting of amelogenin and eight combined DNA index system (CODIS) core STR loci has been constructed and optimized for this real-time human identification study. Reproducible STR profiles of control DNA samples are obtained in 2 h and 30 min with ≤0.8 bp allele typing accuracy. The minimal amount of DNA required for a complete DNA profile is 100 copies. To critically evaluate the capabilities of our portable microsystem as well as its compatibility with crime scene investigation processes, real-time STR analyses were carried out at a mock crime scene prepared by the Palm Beach County Sheriff's Office (PBSO). Blood stain sample collection, DNA extraction, and STR analyses on the portable microsystem were conducted in the field, and a successful “mock” CODIS hit was generated on the suspect's sample within 6 h. This demonstration of on-site STR analysis establishes the feasibility of real-time DNA typing to identify the contributor of probative biological evidence at a crime scene and for real-time human identification. 相似文献
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Richard F. Kaufman 《后苏联事务》2013,29(1):9-31
This paper discusses the recent official U.S. intelligence estimates indicating a slowdown in the growth rates of Soviet defense spending and military procurement since 1976. The Central Intelligence Agency's explanation for the slowdown is examined. Questions are raised about two of the factors, economic constraints and technical difficulties, and the implications of the CIA's explanation for theories about the relationship of the defense sector to the rest of the economy are considered. The author offers as a possible alternative explanation of the slowdown a change in Soviet military investment priorities de-emphasizing strategic offensive weapons. Journal of Economic Literature, Classification Numbers: 052, 114, 124. 相似文献
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Richard Grossman 《The Journal of peasant studies》2013,40(1):80-99
This article explores the creation of a nationalist identity in Nicaragua during the guerrilla war of Augusto Sandino against the occupying United States. The social base of Sandino was the mountain peasants of northern Nicaragua; a social sector usually described as unlikely to become the creators and carriers of a national identity. Yet by using a gendered and familial discourse, which described Nicaragua as the ‘madre patria’ (mother homeland) and the members as his army as brothers, Sandino was successful in activating strong nationalist feelings amongst his peasant followers. The article examines both Sandino's discourse, and how it was interpreted by his peasant followers. It is this attempt to bring their perspectives into the discussion that contributes to this new assessment of the construction of a national identity in Nicaragua. 相似文献
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Richard H. Immerman 《Intelligence & National Security》2013,28(2-3):159-181
This article argues that analytic practices and processes within the US intelligence community have undergone far more fundamental reform than the public or scholarly communities recognize. It identifies the dimensions of this ‘Analytic Transformation’ and explains the reasons for optimism about the future. 相似文献
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