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Our present inability to rapidly, accurately and cost-effectively identify trace botanical evidence remains the major impediment to the routine application of forensic botany. Grasses are amongst the most likely plant species encountered as forensic trace evidence and have the potential to provide links between crime scenes and individuals or other vital crime scene information. We are designing a molecular DNA-based identification system for grasses consisting of several PCR assays that, like a traditional morphological taxonomic key, provide criteria that progressively identify an unknown grass sample to a given taxonomic rank. In a prior study of DNA sequences across 20 phylogenetically representative grass species, we identified a series of potentially informative indels in the grass mitochondrial genome. In this study we designed and tested five PCR assays spanning these indels and assessed the feasibility of these assays to aid identification of unknown grass samples. We confirmed that for our control set of 20 samples, on which the design of the PCR assays was based, the five primer combinations produced the expected results. Using these PCR assays in a 'blind test', we were able to identify 25 unknown grass samples with some restrictions. Species belonging to genera represented in our control set were all correctly identified to genus with one exception. Similarly, genera belonging to tribes in the control set were correctly identified to the tribal level. Finally, for those samples for which neither the tribal or genus specific PCR assays were designed, we could confidently exclude these samples from belonging to certain tribes and genera. The results confirmed the utility of the PCR assays and the feasibility of developing a robust full-scale usable grass identification system for forensic purposes. 相似文献
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This paper analyses the challenges facing the Partido de la Revolución Democrática (PRD) administration when it became the first elected government of the Federal District in 1997. Through a daily review of press coverage between December 1997–December 2000, complemented by intensive interviewing during summer 1999, five areas of policy-making activity are analysed and evaluated. The policies entrained and their outcomes show significant advances in decentralization, devolution, and intergovernmental liaison, as well as modest improvements in environmental contamination and reduced crime, although they did not meet the high expectations generated during the Cárdenas campaign for election. However, the fresh image and invigorated confidence that his replacement Rosario Robles brought to the PRD was key in the PRD's success in the July 2nd 2000 DF elections won by López Obrador. The new administration will have to confront a more plural government structure, including five of the sixteen delegaciones and an evenly divided Legislative Assembly. López Obrador has a full six years in which to prove that a left-of-centre political party is capable of developing a 'Third Way' of governance in the DF. 相似文献
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Ian Ward 《The Modern law review》1994,57(2):315-329
Jacques Derridu, The Other Heading: Reflections on Today's Europe , translated by P-A Brault and M. Naas 相似文献
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Daniel L. Ward 《The Modern law review》2007,70(2):278-293
Part 36 of the Civil Procedure Rules is an important component of English civil procedure's costs apportioning mechanism and its primary means of encouraging early settlement. Two changes to make Part 36 more attractive to defendants have been proposed. Firstly, public and insured defendants would be exempted from the requirement of payment into court. Secondly, the costs protection given to a claimant that betters its Part 36 offer at trial – indemnity costs assessment and enhanced interest – would apply to defendants' offers. It is argued that the payment into court requirement should be abolished. Claimants do not reject settlement offers over doubts as to the defendant's solvency as this risk remains throughout the litigation. The requirement only deters defendants from using Part 36. Awarding indemnity costs under Part 36 creates perverse incentives. Where bettering a Part 36 offer weakens costs scrutiny there is an additional incentive to expend disproportionate resources in achieving a better result. 相似文献
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Liverpool Law Review - The experience of Covid-19 has taught us many things, not least the consequence of what John Milton termed ‘gibberish law’. Law drafted amidst the ‘throng... 相似文献