Automated PCR setup for forensic casework samples using the Normalization Wizard and PCR Setup robotic methods

Human genome, pharmaceutical and research laboratories have long enjoyed the application of robotics to performing repetitive laboratory tasks. However, the utilization of robotics in forensic laboratories for processing casework samples is relatively new and poses particular challenges. Since the quantity and quality (a mixture versus a single source sample, the level of degradation, the presence of PCR inhibitors) of the DNA contained within a casework sample is unknown, particular attention must be paid to procedural susceptibility to contamination, as well as DNA yield, especially as it pertains to samples with little biological material. The Virginia Department of Forensic Science (VDFS) has successfully automated forensic casework DNA extraction utilizing the DNA IQ(trade mark) System in conjunction with the Biomek 2000 Automation Workstation. Human DNA quantitation is also performed in a near complete automated fashion utilizing the AluQuant Human DNA Quantitation System and the Biomek 2000 Automation Workstation. Recently, the PCR setup for casework samples has been automated, employing the Biomek 2000 Automation Workstation and Normalization Wizard, Genetic Identity version, which utilizes the quantitation data, imported into the software, to create a customized automated method for DNA dilution, unique to that plate of DNA samples. The PCR Setup software method, used in conjunction with the Normalization Wizard method and written for the Biomek 2000, functions to mix the diluted DNA samples, transfer the PCR master mix, and transfer the diluted DNA samples to PCR amplification tubes. Once the process is complete, the DNA extracts, still on the deck of the robot in PCR amplification strip tubes, are transferred to pre-labeled 1.5 mL tubes for long-term storage using an automated method. The automation of these steps in the process of forensic DNA casework analysis has been accomplished by performing extensive optimization, validation and testing of the software methods.     

Assessing the effectiveness and efficiency of a police service: the analytics of operational reviews

Concerns over rising costs have forced police services to balance increasing fiscal and operational pressures. Discussions of the resources and requirements of police, however, are generally only informed by political expediency and traditional practice. Operational reviews (ORs) can be used to document the demands on police, their capacity to respond, and ways in which they can become more efficient and effective. Drawing on an OR of a major urban Canadian police service, this article provides a broad outline of the components of an OR and the analytics that can be used to answer these key questions.     

The Hollywood War Machine

What we call the "Hollywood War Machine" analyzes the production of films within the studio system depicting the glories of US military action within theaters from around the world, spanning the Revolutionary War period to the present. A large majority of films under discussion here can be understood from the perspective of "good wars" that the film industry has strongly favored, especially during the 1990s. This essay reveals an increasing emphasis on the World War II experience as the quintessential "good war" that brings American audiences back to the undistilled benevolence of the US military through its various patriotic and heroic exploits. The undoubtedly powerful impact of the September 11, 2001 events and their aftermath upon the Hollywood War Machine is a topic worthy of further discussion.     

The Caribbean Court of Justice: a learning experience for the International Criminal Court?

This article starts by examining the role of Small States in the development of the International Criminal Court (ICC). It then surveys the functioning and administration of the ICC before proceeding to draw on key lessons for this Court, including on the election of judges and financing, from the Caribbean Court of Justice.