Sequence variation of new alleles at the short tandem repeat D19S253 locus

This paper reports the sequences of two new alleles identified in a population database study on the short tandem repeat D19S253 locus. A Portuguese Caucasian population and a Portuguese African population were studied. Forty-four selected alleles were sequenced and 11 different alleles were found. All the sequenced alleles shown to possess a simple tetranucleotide GATA repeat region structure. The two new alleles, alleles 6 and 16, follow the simple repeat pattern. During paternity investigation casework, 1028 meiosis were analyzed and five isolated genetic incompatibilities detected. In one case, a non-detectable allele with the used set of primers could be the explanation. In the other four cases, single-step mutations could be considered. The mutation rate obtained for this locus was 3.89 x 10(-3).     

Results of the GEP-ISFG collaborative study on two Y-STRs tetraplexes: GEPY I (DYS461, GATA C4, DYS437 and DYS438) and GEPY II (DYS460, GATA A10, GATA H4 and DYS439)

A collaborative exercise was carried out by the Spanish and Portuguese ISFG Working Group (GEP-ISFG) in order to evaluate the performance of two Y-chromosome STR PCR tetraplexes, which include the loci DYS461, GATA C4, DYS437 and DYS438 (GEPY I), and DYS460, GATA A10, GATA H4 and DYS439 (GEPY II). The participating laboratories were asked to type three samples for the eight markers, using a specific amplification protocol. In addition, two control samples, with known haplotypes, were provided. The results obtained by the 13 different participating laboratories were identical, except for two laboratories that failed to type correctly the same two samples for GATA C4. By sequence analyses, two different GATA C4 allele structures were found. One control sample (allele 21) and two questioned samples (allele 22, correctly typed by all the laboratories, and allele 25) presented the following repeat structure: (TCTA)4(TGTA)2(TCTA)2(TGTA)2(TCTA)n, but different from the one found for allele 26 in one sample included in this exercise, as well as in the second control sample (allele 23), namely (TCTA)4(TGTA)2(TCTA)2(TGTA)2(TCTA)2(TGTA)2(TCTA)n. The collaborative exercise results proved that both Y-tetraplexes produce good amplification results, with the advantage of being efficiently typed using different separation and detection methodologies. However, since GATA C4 repeat presents a complex structure, with alleles differing in sequence structure, efficient denaturing conditions should be followed in order to avoid typing errors due to sizing problems.     

What About Syria?

The following is the edited text of the presentations at the September 30, 1999, meeting of the Sadat Forum at Brookings, cohosted by Richard Haass, vice-president and director of Foreign Policy Studies at the Brookings Institution, and Shibley Telhami, the Anwar Sadat Chairfor Peace and Development at the University of Maryland.     

Privacy and intra-familiy communication of genetic information.

The new knowledge (and predictions) created by DNA tests and the family nature of genetic information has already lead to a new problem: the intra-familiar communication of genetic data. This raises questions such as the following. Is there a duty to inform in cases when treatment is possible and the patient does not permit disclosure of genetic results to relatives? Is there an obligation to warn or merely an authorization (that could be used or not)? Could privacy protection be maintain as an individual interest but with some justified violations? A balance needs to be establishes between the interest of privacy and the need to disclose secret information.     

Fatal intoxication with tianeptine (Stablon)

Tianeptine (Stablon^®), although structurally similar to tricyclic antidepressants, acts by enhancing the reuptake of serotonin. A fatal case is presented involving a 26-year-old man, found lying in bed with a “mushroom of foam” around his mouth. Empty blister packs of Stablon^® and a suicide note were found next to the body. A liquid–liquid extraction procedure with n-hexane: ethyl acetate and n-hexane: 2-propanol, followed by LC-DAD-MS analysis, using positive mode electrospray ionization was performed. The detection limit was 0.001 μg/mL. The toxicological results revealed the following tianeptine concentrations in the post-mortem samples: blood 5.1 μg/mL; urine 2.0 μg/mL; liver 23 μg/g; stomach contents 22 mg. Femoral blood analyses also revealed an ethanol concentration of 0.53 g/L. The present method was also developed and validated for the other post-mortem specimens, since no previous published data had confirmed the post-mortem distribution of tianeptine. The absence of other suitable direct causes of death (macroscopic or histological) and the positive results achieved with the toxicological analysis led the pathologist to rule that death was due to an intoxication caused by the suicidal ingestion of tianeptine in combination with alcohol.     

Depression and substance use in two divergent high school cultures: A quantitative and qualitative analysis

Research has generally concluded that adolescent depression and substance use are strongly interrelated, but has rarely considered how this relationship may vary across diverse populations. In this study, we used quantitative and qualitative methods to explore the relationships among depression and cigarette, alcohol, marijuana, and harder drug use across two culturally disparate environments: a suburban and an inner-city high school. Our sample included 164 suburban and 242 inner-city high school students. The students completed Kovacs' Children's Depression Inventory of 1985 and substance use measures derived from various sources. In-depth semistructured interviews were conducted with subjects who scored in the top 10% of the CDI (N=19) from both schools. Our quantitative findings indicated a positive association between depression and cigarette, marijuana, and harder drug use among the suburban students, and no association between depression and the use of any substances for the urban students. There were no significant differences in levels of reported depression across samples. However, with the exception of marijuana use, suburban students reported greater involvement in substance use than urban students. Our qualitative analyses suggest that across-school differences in the relationships among depression and substance use may be related to the varied meanings of depression and substance use that are informed by cultural context.The research was funded by the National Institute of Drug Abuse (NIDA), Grant No. 1 R01 DA-06844, Perry London, principal investigator. The views, opinions, and findings contained in their article are not to be construed as NIDA's position or policy.B.A. from University of California, Berkeley, and Ed.D. from the Graduate School of Education, Harvard University. Research interests include the phenomenology of high-risk behavior and social development among urban adolescents.B.A. from Mount Holyoke College and Ed.M. from the Graduate School of Education, Harvard University. Research interests include the relationships among high-risk behavior, personality variables, and cultural context.B.A. from Michigan State University, M.A. from University of Minnesota-Duluth, and Ed.D. from Harvard Graduate School of Education. Research interests include integrating quantitative and qualitative methods in social science research and the phenomenology of high-risk behavior.Research interests included adolescent high-risk behavior and ethnic and religious identity development. Deceased June 1992.     

European Legal Method in Denmark and Sweden—Using Social Science Theory and Methodology to Describe the Implementation of EU Law

This article presents data from a study in which national bureaucrats working in the fields of taxation and food law in Sweden and Denmark are asked which legal sources and methods of interpretation they use when implementing EU law. The purpose is to contribute to the discussion about European legal method by using social science methodology. National agencies and authorities in the fields of taxation and food law face a ‘multilayered’ or ‘multiprincipal’ reality in which there is room for policy choices. The answers given by the interviewees speak of a plurality of legal sources, a situation where bureaucrats are becoming reluctant lawmakers instructing others on how EU law is to be applied and where bureaucrats find it necessary to found their decisions on what colleagues within the authority or from other Member States have said about how EU law should be applied.