A method for preparation of DNA suitable for medico-genetic studies from heparinized blood samples

Heparin traditionally used as an anticoagulant for blood preparations is one of the most powerful inhibitors of the polymerase chain reaction. The molecular genetic analysis for the purpose of forensic medical expertise encounters difficulties when DNA preparations obtained from heparinized samples have to be used. In our practical work, we were faced with the necessity to use heparin-treated blood samples. It turned out impossible to eliminated heparin from DNA isolated from these samples by the known methods. In order to obviate this difficulty, we have developed a special method for obtaining DNA from heparinized blood preparations suitable for the purpose of forensic medical expertise. The new technique includes the stages of preliminary extraction and purification of the blood cell fraction.     

Optimization of the biological microchip for genotyping the AB0 locus: analytical aspects

The present work continues the search for methodological options facilitating the improvement and optimization of the biological microchip designed for genotyping the AB0 locus. It was shown in an earlier study designed to test a prototype biological microchip using a reference set of preparations with the known group specificity that under certain conditions some cells of the biochip appear to generate artifact hybridization signals that tend to make the results of genotyping either incorrect or difficult to interpret. We performed the correction of the molecular structure of DNA probes of the prototype biochip for the purpose of optimization of their hybridization potency. In addition, we developed and synthesized new DNA probes and designed new variants of the biochip. The experimental analysis of hybridization properties of all DNA probes thus obtained was carried out for the final choice of the most promising options suitable for the creation of the optimized biochip.     

Does Hypocrisy Matter? National Reputational Damage and British Anti-Corruption Mentoring in the Balkans

In the late 2000s, numerous prominent public commentators raised concerns that corruption scandals were harming Britain’s ability to play a leadership role in anti-corruption initiatives abroad. With a view to contributing to critical criminological scholarship on international policy transfer and double standards in criminal justice policy and practice, this article explores the extent to which reputational damage curtailed Britain’s appeal as an anti-corruption mentor in South-East Europe during the 2000s. Challenging the common presumption that stronger states tarnished by corruption scandals will face ‘hypocricy costs’ abroad, this article finds that a range of factors work to insulate stronger states from the potential ramifications of reputational decline.     

Polymorphism of structural genes of human mitochondrial DNA for forensic medical personal identification

An experimental study is described, which deals with polymorphism of nucleotide sequences of three structural genes of mitochondrial genome, i.e. of the 3d, small 4th, and 6th subunits of the NADN dehydrogenase complex (ND3, ND4L and ND6) sampled from Russian population. The genetic primary structure was analyzed in 63 unrelated individuals. The investigated locuses were shown to possess a pronounced polymorphism. A total of 19 polymorphic positions were detected in the ND3, ND4L and ND6 gene region within the studied sampling. Besides, a possibility is demonstrated in the paper that the mtDNA structural genes can be used as additional identification markers in the forensic experimental typing of the mtDNA control region.     

Design of the automatic system of processing data on mitochondrial DNA "mDNAbase" sequestration for expert identification of the unidentified bodies under the conditions of mass reception of human remains

The authors consider utilization of software which provides indirect personality identification by establishment of biological kinship using a comparative analysis of nucleotide sequences (NS) of mitochondrial DNA (mtDNA) in unidentified bodies and suspected relatives of these victims. The software supports data bases with information on NS. To secure automatic search there are modes of cross-processing. Results of identification are formed as lists of exclusions and tables with calculated frequencies of mitotypes of identification objects as well as protocols of a comparative analysis of nucleotide position in mtDNA sequences under comparison. The proposed computer approach is a new highly effective tool for identification of personality based on data on mtDNA analysis in conditions of stream information processing under mass reception of unidentified bodies.     

Use of computer software for indirect molecular genetic identification of unidentified bodies

The authors discuss the development and use of computer software for realization of indirect DNA identification, based on identification of biological relation. Estimated algorithms of this method are based on regularities of parental signs inheritance by children and consist in comparative analysis of allele states of nuclear DNA typed locuses in unidentified bodies and probable parents of these dead subjects and subsequent estimation of the coefficients of the likelihood of hypotheses of their probable blood relationship. Available software maintain the database with identification characteristics of VNTR and STR locuses, HLA DQA1 locuses, and PM system (potential set of 23 locuses). The results of identification are presented as lists of exclusions and tables with estimated likelihood coefficients for the probability expert evaluation of relationship. The suggested computer-aided method of indirect identification is a new highly effective tool for personality identification by chromosome markers under conditions of mass information processing in examinations of unidentified corpses.     

Typing of STR locus in biological objects with pronounced degeneration: analysis of DNA in exhumed remains of victims of war actions

The authors describe some specific features of enzymatic amplification typing of DNA preparations obtained from degraded tissues of remains of humans, which were brought from regions of war actions in the Chechen Republic. Special attention is paid to the specific artefact of polymerase chain reaction, for the first time detected by the authors in examinations of the above-mentioned objects. This so-called "ladder" effect manifesting by simultaneous nonspecific amplification of many variants of allele fragments of the STR locus on the individual DNA matrix, which can be erroneously interpreted as an evidence of mixed DNA preparation, is apparently characteristic of individual objects with pronounced degradation of biological material. Such phenomena were observed in typing of STR locuses in biological tissues subjected to biological, thermal, and physicohemical degradation. The phenomenon was studied in detail.     

Computer analysis of face and skull size by "quantitative verbal portrait" method

The aim of this study was to develop a "quantitative verbal portrait" method on the basis of universal measured signs of the face and skull. The database includes 90 cases with expert evaluation of photocompatibility with proven identity. Four groups of signs were investigated: DX and DY--the width and height between accurately fixed concrete craniometrical (facial) points and the so-called RX and RY indices--the ratio between distances within the framework of the same direction. The proposed scheme of evaluation helps compare the photograph of a live human and the skull. For evaluating the degree of identity, one should have a summary characterization of all selected signs for the skull and compare it with the sum on the photograph; sometimes it is possible to rule out the identity of the photograph and the skull by the absolute size, e.g., a very large skull cannot belong to a human with a very little face. The score for each group of signs is used for analysis of the information for detecting the similarities. Accumulation and comparative analysis of two information flows are possible: database on portraits of lost subjects and database on graphic images of the skulls of unidentified corpses.