Genetic markers in human bone: I. Deoxyribonucleic acid (DNA) analysis.

Deoxyribonucleic acid (DNA) was isolated from a number of spongy and compact human bone tissue specimens, and the yield was estimated on a "per milligram of starting tissue" basis. DNA was, in addition, isolated from a number of corresponding blood and bone tissue specimens. Spectrophotofluorometry and ethidium bromide visualization on minigels were used to estimate the quantity and degree of degradation of DNA. The DNA from several blood-bone pairs is shown to give concordant restriction fragment length polymorphism (RFLP) typing results by two different typing protocols with five different single-locus probes. DNA from several additional blood-bone pairs is shown to give concordant results for human leucocyte antigen (HLA)-DQ alpha phenotypes following polymerase chain reaction (PCR) amplification and hybridization to specific allele-specific oligonucleotide (ASO) probes, and for the variable numbers of tandem repeats (VNTR) length polymorphisms 3' to the human apolipoprotein B (APOB) gene following PCR amplification with specific primers and analysis of the products by electrophoresis and ethidium bromide visualization.     

Candidates' Ideological Locations,Abstention, and Turnout in U.S. Midterm Senate Elections

Many spatial models of voting suggest that citizens are more likely to abstain when they feel indifferent toward the candidates or alienated from them. In presidential elections, previous research offers evidence that alienation and indifference affect individuals' probabilities of voting. We find evidence that indifference and alienation also affect the decision to vote in midterm Senate elections, a context not previously explored. These individual-level effects imply that candidates' ideological locations should influence aggregate turnout by affecting the proportions of citizens who feel indifferent toward or alienated from the candidates. Our aggregate-level analysis supports this (at least in contests featuring two previous and/or future members of Congress). Our findings underscore the importance of the electoral context for understanding citizen behavior and suggest that elections featuring at least one centrist candidate may be normatively appealing since they stimulate participation.     

Interstate Cooperation: The Roles of the State Attorneys General

State attorneys general are major participants in intergovernmentalrelations in the United States. This article presents six modelsthat may explain their interactions with each other and teststhree hypotheses associated with two models. Their interactionscomport with the cooperative and the innovation-diffusion models.Only limited evidence supports the hostility model and its associatedhypothesis. No evidence supports the mercantilist and competitivemodels. The response from only one attorney general suggestedhis actions are based on the benign neglect model. The conclusionis drawn that the cooperative activities of the attorneys generalhave produced a more harmonious federal system in terms of enforcementpolicies.     

When Did Health Care Become a Campaign Issue?

Most people mistakenly assume that health care first became a major political issue in 1945 because President Harry S. Truman’s special address to Congress on Nov. 19 of that year marked the first time a sitting president publicly endorsed a national health-care program. But the question of whether—or to what extent—it is the responsibility of government to subsidize health care for its citizens has been around for a much longer amount of time. Now that health care has become a major focus of domestic political debate, especially in light of the impending presidential election, this article, modified from an entry in the forthcoming Encyclopedia of Campaigns, Elections, & Electoral Behavior (Sage Publications), serves to inform the reader of the origins and history of health care as a campaign issue.