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Grassin Delyle S Duverneuil-Mayer C Abe E Mathieu B Lorin De La Grandmaison G Charlier P Alvarez JC 《Forensic science international》2008,178(2-3):e19-e21
The dead body of a 44-year-old woman, previously known for depression and alcoholism, has been discovered at her place of residence by her husband. A forensic autopsy has been carried out. The results indicated unspecific histological lesions (alveolar oedema, liver steatosis and interstitial nephritis) but did not reveal any apparent cause of death. Several boxes of medicines have been found near the body, justifying a toxicological analysis. This has been performed on peripheral blood and urine samples using liquid chromatography with diode array and mass spectrometric detections, in conjunction with gas chromatography coupled with mass spectrometry. Ethanol has been found (1.24 g/L in blood, 2.63 g/L in urine and 1.33 g/kg in gastric content), as well as therapeutic concentrations of meprobamate (14.1mg/L) and low concentrations of nordazepam (0.12 mg/L) in blood. On the other hand, particularly high levels of labetalol, a widely used beta-blocker, have been found both in blood (1.7 mg/L) and urine (20.2mg/L), which led us to measure labetalol levels in available viscera samples (liver, heart, kidney, and lung) and gastric content. Measured concentrations were 14.2 microg/g, 7.8 microg/g, 5.4 microg/g, 5.2 microg/g and 31.1 microg/g, respectively. We describe here the first report of a fatal intoxication attributed to labetalol that is linked to its acute toxicity, with tissue distribution of this beta-blocker. 相似文献
83.
La Fond JQ 《International journal of law and psychiatry》2008,31(2):158-171
No other country has taken the idea of the liberal state - a government that relies on the rule of law to limit state power and maximize individual liberty - as seriously as the United States. But now many states are manipulating the government's civil commitment authority to indefinitely confine sex offenders who have served their criminal sentences and are entitled to their freedom in the community. This new system of preventive detention masquerading as civil commitment poses an ominous threat to individual liberty and to the continued vitality of the liberal state. 相似文献
84.
Bones and teeth often represent the only sources of DNA available for identifying human remains. DNA in bones and teeth is generally better preserved than that in soft tissues because of the presence of hard connective tissue with a high level of calcium. Because of the extensive mineralisation, the choice of an efficient DNA extraction procedure is important to minimise the sampling of a high level of minerals and to remove polymerase chain reaction (PCR) inhibitors. Some protocols are available for DNA extraction from bones and teeth as part of the Qiagen EZ1 DNA Investigator Kit using the EZ1 Advanced XL automated purification platform. To improve the efficiency of DNA extraction from skeletal remains, the present study focuses on a modification to these already available protocols. In this study, different bones and teeth collected between 1 and 50 years after death were subjected to DNA extraction using the standard EZ1 protocol, a supplementary protocol, and a modified protocol. The modified approach included a decalcification step, whereas the Qiagen protocols worked directly on non-decalcified powder. In all three procedures, 150 mg samples were used for DNA extraction. We evaluated the quantity of DNA recovered from samples, the presence of any PCR inhibitors co-extracted, the level of DNA degradation, the quality of short tandem repeat (STR) profiles, and the reproducibility of the modified procedure. When compared with the other protocols, the modified protocol resulted in the best recovery of DNA that was free of PCR inhibitors. Additionally, the STR profiles were reliable and of high quality. In our opinion, the decalcification step increases DNA recovery by softening tissues, which allows lysis solutions to act more effectively. Furthermore, the use of two lysis solutions and the variation added to the EZ1 purification step allow for DNA recovery with quality and quantity superior to those of the previously available Qiagen-based protocols. These findings may be helpful solutions to the problems commonly encountered when dealing with difficult samples, such as bones and teeth.
Key points
- Bones and teeth often represent the only sources of DNA for identifying human remains.
- The choice of an efficient DNA extraction procedure is important for maximizing DNA recovery and removing PCR inhibitors.
- This study focuses on modifications to the previously available Qiagen-based protocols.
- The modified protocol enabled the best recovery of DNA, and both quality and quantity were superior to those of the previously available Qiagen-based protocols.
- The STR profiles obtained from samples extracted using the modified protocol were reliable and of high quality.
85.
This paper investigates agglomeration externalities on the productivity of informal household businesses in Vietnam. Although the paper finds heterogeneous impacts of agglomeration on the productivity of informal firms between urban and rural settings and across industries, it is found that technology transfers are not an agglomerative force for the informal sector of Vietnam. The disaggregation of local scale externalities into city types reveals that the highest developed cities in Vietnam may either be low-quality agglomeration or go beyond their optimal scale for the informal sector in some industries. 相似文献
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David De Young De La Marck 《Intelligence & National Security》2013,28(1):21-40
De Gaulle's relationship with his secret intelligence and subversive services, the Bureau central de renseignements et d'action (BCRA) headed by Colonel Passy, as well as with British intelligence is examined in the light of the now declassified archives of the Special Operations Executive (SOE) and the BCRA. These archives reveal that de Gaulle failed to interest himself seriously in secret intelligence or subversion before the arrival of Jean Moulin in London in October 1941. De Gaulle's subsequent relationship with the BCRA and British intelligence was defined by an obsessive need for political control, which only served to compromise the BCRA's otherwise successful collaboration with British intelligence. 相似文献
90.
Felice Carabellese Donatella La Tegola Gabriele Rocca Alessandro Catamo Domenico Martinelli 《The journal of forensic psychiatry & psychology》2014,25(5):574-583
An ample volume of research evidence supports the conclusion that severe mental illness is correlated with violent behavior. While episodes of violent behavior are included in medical notes, not all episodes of violent behavior are officially reported to the police, even when they are actual crimes. We conducted a retrospective study on the already existing clinical files of four public psychiatric outpatient facilities, based in Southern Italy, in order to assess how many cases of violent behavior are actually reported and which variables are more frequently connected with the decision to report. The data shows that the episodes known to the health services, in number and seriousness, vastly outnumber the complaints actually made towards their patients. 相似文献