Automated extraction of DNA from reference samples from various types of biological materials on the Qiagen BioRobot EZ1 Workstation

We have validated and implemented a protocol for DNA extraction from various types of biological materials using a Qiagen BioRobot EZ1 Workstation. The sample materials included whole blood, blood from deceased, buccal cells on Omni swabs and FTA Cards, blood on FTA Cards and cotton swabs, and muscle biopsies. The DNA extraction was validated according to EN/ISO 17025 for the STR kits AmpF?STR^® Identifiler^® and AmpF?STR^® Yfiler^® (Applied Biosystems). Of 298 samples extracted, 11 (4%) did not yield acceptable results. In conclusion, we have demonstrated that extraction of DNA from various types of biological material can be performed quickly and without the use of hazardous chemicals, and that the DNA may be successfully STR typed according to the requirements of forensic genetic investigations accredited according to EN/ISO 17025.     

Daily and Compulsive Internet Use and Well-Being in Adolescence: A Diathesis-Stress Model Based on Big Five Personality Traits

This study examined the associations between adolescents’ daily Internet use and low well-being (i.e., loneliness, low self-esteem, and depressive moods). We hypothesized that (a) linkages between high levels of daily Internet use and low well-being would be mediated by compulsive Internet use (CIU), and (b) that adolescents with low levels of agreeableness and emotional stability, and high levels of introversion would be more likely to develop CIU and lower well-being. Data were used from a sample of 7888 Dutch adolescents (11–21 years). Results from structural equation modeling analyses showed that daily Internet use was indirectly related to low well-being through CIU. In addition, daily Internet use was found to be more strongly related to CIU in introverted, low-agreeable, and emotionally less-stable adolescents. In turn, again, CIU was more strongly linked to loneliness in introverted, emotionally less-stable, and less agreeable adolescents.

Heteronormative Foundations of Modern Citizenship in Early-twentieth-century Denmark

During 1906–1907 the Great Morality Scandal shocked Copenhagen’s general public by disclosing a handful of respectable bourgeois men as secret homosexuals. This article examines how the city’s working-class newspapers attempted to politicize the scandal by questioning the bourgeoisie’s privileged citizen status on the grounds of their supposed homosexuality, and by claiming that the partly disenfranchised working classes were worthy of full citizen status since they were heterosexual members of society. The article draws upon a theoretical framework that includes queer theoretical critiques of heterosexual norms and ideas of citizenship as a series of performative acts. It argues that working-class writings about the Great Morality Scandal constructed access to full Danish citizenship around the embodiment and enactment of heterosexual desire. They thus contributed to the foundations of modern Danish citizenship as distinctly heteronormative.     

The cyber frontier and digital pitfalls in the Global South

How does digitalisation lead to new kinds of global connections and disconnections in the Global South? And what are the pitfalls that accompany this development? Much of the policy literature on digitalisation and development has focused on the importance of connecting developing countries to digital networks. Good connection to digital networks may have a fundamental impact on societies, changing not only how individuals and businesses navigate, operate and seek opportunities, but also as regards relations between government and the citizenry. However, the rapid pace of this development implies that digital technologies are being put to use before good, functional regulatory mechanisms have been developed and installed. The resultant shortcomings – in state mechanisms, institutions, coordination mechanisms, private mechanisms, general awareness, public knowledge and skills – open the door to new kinds of vulnerabilities. Herein lie dangers, but also opportunities for donor/recipient country exchange. Instead of adding to the already substantial literature on the potential dividends, this article examines a less studied issue: the new societal vulnerabilities emerging from digitalisation in developing countries. While there is wide agreement about the need to bridge the gap between the connected and the disconnected, the pitfalls are many.     

The ITS Directive: More than a timeframe with privacy concerns and a means for access to public data for digital road maps?

Roads are ever more congested, pollution keeps rising and traffic-related deaths remain at unacceptable levels. It is clear that society’s needs with regard to transportation and mobility have become unsustainable. Intelligent Transport Systems (ITS) are often heralded as a potential solution to this problem, yet have still to yield tangible results. The EU has, however, adopted the ITS Directive, aiming for an EU-wide implementation of ITS solutions. Three questions are raised. First, can the ITS Directive really provide for the required substantial provisions in this field? Second, as ITS solutions are often deemed to be pervasive and intrusive, how does the ITS Directive interact with the EU legal framework on privacy and data protection? Third, given the involvement of private commercial entities in the field of providing road, traffic and travel data, can a public–private partnership be found to allow for the re-use of both public and private sector data in ITS solutions?     

Public interest versus interest groups: a political economy analysis of allowance allocation under the EU emissions trading scheme

In a theoretical analysis, we use a common agency model to show that lobbying by energy-intensive sectors covered under an emissions trading scheme (ETS) shifts the regulatory burden of an economy-wide emission constraint to sectors outside the ETS. The emission tax on the latter becomes inefficiently high such that lobbying does not only induce burden shifting but also efficiency losses. A complementary empirical analysis for a cross section of German firms under the EU emissions trading scheme supports our theoretical result on the role of lobbying on allowance allocation.     

Torture in Israel

Conclusion The High Court's verdict is a major step forward insofar as that the existing institutionalized GSS torture practices are declared unlawful and are no longer possible in any institutionalized form. It appears, however, likely that Israel will attempt to reintroduce the legal use of different forms of “physical pressure” under specific circumstances. The legality of these forms in Israel is then likely to be tested by a High Court of Justice which is still clearly ambivalent and far from unequivocal in declaring all forms of physical means during interrogations to be unlawful. Whereas the Court's ruling declaring the present guidelines and various practices of “moderate physical force” unlawful is a positive step from a human rights perspective and in the context of the Middle East peace process, its significance might thus eventually turn out to be less of a breakthrough than initially thought. The international community has ample reason to continue to follow Israel critically regarding possible infractions of the Convention and the ICCPR. I would like to thank Jeroen Gutter and Ian Seiderman for their assistance with this article.     

Non-Zero-Sum Leadership Games: Is Facilitative Leadership a Win-Win?

According to the concept of facilitative leadership, leaders can strengthen their own leadership by strengthening the other players in the game. However, quantitative empirical evidence that such facilitative leadership processes can lead to win-win situations has been lacking. This article develops a way to detect such non-zero-sum leadership games building on an index constructed to measure political leadership in surveys. The technique is applied to a case study focusing on Regional Mayors in Denmark, and the empirical findings clearly demonstrate that, in this case, the leadership game indeed resembles a non-zero-sum pattern.     

A report of the 2000 and 2001 paternity testing workshops of the English speaking working group of the international society for forensic genetics

During the last 10 years, the English Speaking Working Group (ESWG) of the International Society for Forensic Genetics (ISFG) has once a year arranged a Paternity Testing Workshop in which blood samples as well as a questionnaire concerning laboratory strategies were distributed to the participating laboratories. In 2000 and 2001, paper challenges were included in the workshops. Here, we present the results of the 2000 and 2001 Paternity Testing Workshops. The numbers of participating laboratories were 33 (2000) and 36 (2001). A total of 36% (2000) and 31% (2001) of the laboratories submitted typing results of variable number of tandem repeats (VNTRs) investigated with restriction fragment length polymorphism (RFLP) and single locus probes (SLPs). A total of 91% (2000) and 86% (2001) submitted typing results of polymerase chain reaction (PCR) based systems. Typing errors occurred in 0.3% of the submitted PCR-based results in 2000 and in 0.1% in 2001. The results of the paper challenges showed a high degree of variation in the formulas used for calculation of the weight of evidence of rare events such as inconsistencies or possible silent alleles. The majority of the laboratories used the same formulas for calculations of frequently occurring events.     

Photogrammetric Documentation of Regions of Interest at Autopsy—A Pilot Study

In this pilot study, the authors tested whether photogrammetry can replace or supplement physical measurements made during autopsies and, based on such measurements, whether virtual computer models may be applicable in forensic reconstructions. Photogrammetric and physical measurements of markers denoting wounds on five volunteers were compared. Virtual models of the volunteers were made, and the precision of the markers' locations on the models was tested. Twelve of 13 mean differences between photogrammetric and physical measurements were below 1 cm, which indicates that the photogrammetric method has a high accuracy. The precision of the markers' location on the models was somewhat less, although the method is still promising and potentially superior to the current procedures used for reconstructions. The possibility to measure any distance on a body, even after the autopsy is concluded and the corpse is no longer available, is one of the biggest benefits of photogrammetry.