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131.
132.
The purpose of this study was to compare the effectiveness of the QIAGEN QIAamp Stool Mini Kit against a standard phenolchloroform procedure for the extraction, quantitation, and STR-typing of human nuclear DNA from human feces. Stools from six subjects were sampled by swabbing and excision. Samples extracted with the QIAamp kit gave a wide range of DNA yields, whereas those extracted by the organic method yielded no DNA. DNA was not recovered from one subject's stools by either procedure. The QIAamp extracts were amplified with the Profiler Plus and COfiler kits, and PCR inhibition was observed with DNA extracts that were further concentrated. Substitution of water or TE-4 for the QIAamp elution buffer eliminated most, if not all, of the inhibition. A modified QIAamp procedure was used to extract thirty samples, which were subjected to one of five environmental conditions. DNA was recovered from all of these samples, and typing results were obtained on 93% of the samples. 相似文献
133.
Robert E. Roberts Catherine R. Roberts Irene G. Chen 《Journal of youth and adolescence》2001,30(1):1-18
The objective of the study was to examine the association between disturbed sleep and the functioning of adolescents, in particular cumulative effects on functioning across multiple life domains. Data from a large, school-based survey (n = 5,423) were used to examine the association between eight indicators of psychological, interpersonal, and somatic functioning and symptoms of insomnia and hypersomnia among adolescents. The strongest correlates of insomnia were disturbed mood (OR = 5.9), fatigue (OR = 7.4), and suicidal ideation (OR = 3.4). The same pattern held for hypersomnia, although the associations were not as strong for fatigue (OR = 6.8), mood disturbance (OR = 3.5), and suicide ideation (OR = 2.8). Youths who experienced both insomnia and hypersomnia had greater odds of psychological, interpersonal, and somatic dysfunction than youths who had experienced only one sleep disturbance. Cumulative effects of sleep problems were pronounced, with 27.6% of those with hypersomnia, 41.7% of those with insomnia, and 59.2% of those with both sleep problems reporting three or more types of dysfunction. The results suggest that adolescents experiencing disturbed sleep also experience a range of deficits in functioning. Further, youths with disturbed sleep are more likely to manifest multiple deficits in functioning. From the available evidence, however, it is not possible to specify what the causal pathways are. What are needed are cohort studies designed to examine the association of disturbed sleep and functional impairment among adolescents prospectively. 相似文献
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A defining feature of the modern US Senate is obstruction. Almost all pieces of legislation considered in the Senate are affected either directly or indirectly by obstruction. Obstruction takes many forms in the modern Senate, but one of the most prevalent, yet least studied, is the hold. Using a newly created dataset on Republican Senate holds, we cast light on this important practice. Our results suggest that a variety of factors including timing, party status, and a senator's voting record are related to both the prevalence of holds and the success of legislation subject to holds in the Senate. 相似文献
137.
Roberts T. Jones 《政策研究评论》1987,6(4):777-781
The U.S. Department of Labor's "Work Force 2000" project has identified long-term trends that will affect future employment and training policy. These include demographic trends such as a decline in the pool of young labor market entrants and an increase in women, minority, and immigrant workers; the continued shift from goods-producing industries to the service sector; and continued technological innovation in response to increased international competition. These projected changes in the labor market and workplace present a national challenge and an opportunity to tackle the longstanding problems of disadvantaged workers. Cooperative public and private programs to eliminate illiteracy and increase job skills and competencies, ease labor mobility and provide retraining, and attack underlying social problems that support the cycle of welfare dependency and unemployment are needed to provide the workforce America needs for the future. 相似文献
138.
William Roberts Clark 《国际研究季刊》1998,42(2):245-270
Two analytical distinct approaches to the study of domestic politics have been referred to as the "new institutionalism." The fundamental difference between the two brands of institutionalism can be seen in the way they handle the relationship between "agents" and "structures.""Structure-based" approaches to institutions give ontological primacy to structures and view agents as being constituted by them. "Agency-centered" approaches view human agents as ontologically primative and view institutions as structures that are created by goal-maximizing individuals. The two approaches are compared, with special attention given to the way they treat the preferences that actors hold. I argue that contrary to arguments made by many structure-based theorists, the agency-centered approach is capable of contributing to discussions regarding the sources of actor preferences. A limited information model of the strategic interaction between workers and capitalists is used to demonstrate ways in which the agency-centered approach can begin to make preferences endogenous. 相似文献
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140.
This paper describes the use of physical anthropological methods of analysis in attempting to identify an historical figure in England. The historical background to the examination is detailed and reasons for the belief that the skeletal remains excavated from the site were those of a named person, Margaret Clitherow, are discussed. Analysis of age, sex and manner of death indicated that the remains were not those of Margaret Clitherow. 相似文献