蛋鸡血清与卵黄中禽流感病毒抗体的相关性

用禽流感 (AI)H9N2油乳剂灭活疫苗免疫非免疫蛋鸡 ,并于免疫后第 0、3、5、7、9、11、13、15、17、19、2 1、30d分别测定血清及卵黄中的AI血凝抑制试验 (HI)及琼脂扩散凝集试验(AGP)抗体。结果显示 ,免疫后第 7d卵黄中即可检出HI及AGP抗体 ,以后逐渐上升 ,在免疫后第 19～ 2 1d达高峰值 ,与血清的HI抗体水平接近 ,差异不显著 (P >0 .0 5 )。表明通过对鸡蛋卵黄AIV抗体检测 ,可以进行AI的疫情监测 ,但卵黄抗体检测具有一定的滞后性     

猪圆环病毒Ⅰ型ORF1基因的克隆及其在大肠杆菌中的表达

根据GenBank中猪圆环病毒Ⅰ型(PCV1)ORF1基因序列,设计合成了1对引物,对PCV1 ORF1基因进行PCR扩增,将扩增出的ORF1基因(939 bp)克隆入pMD18-T载体,将筛选获得的重组质粒命名为pMD-ORF1。测序分析表明,克隆的ORF1与广东分离株DQ659154的核苷酸序列同源性高达99.6%,推导的氨基酸序列同源性为99.4%。将ORF1双酶切产物插入原核表达载体pET-41a,得到的重组子命名为pET-ORF1。用IPTG进行诱导表达,收集菌液进行SDS-PAGE和Western-blot分析,结果表明,PCV1ORF1在pET-41a中获得了高效融合表达,其表达蛋白的分子质量约为67.6 ku。     

噬菌体在细菌性疾病诊断和治疗中的应用

从重组噬菌体法、生物扩增法、噬菌体触发的离子级联感应技术及耐药性检测4个方面阐述了噬菌体在病原菌检测中的作用;从细菌分型的角度阐述了噬菌体在病原菌分型中的应用;从活噬菌体、裂解酶、药物载体3个方面陈述了噬菌体在细菌性疾病治疗方法中的研究进展,并分析了噬菌体在应用中的优缺点,展望了今后的研究和应用前景。     

中国人口老龄化对经济发展的影响机制及对策研究

人口老龄化与少子化已经成为我国人口结构的基本特征,将对我国经济高质量发展产生深远的影响。最新公布的全国第七次人口普查数据显示我国人口老龄化呈现四个特征：人口老龄化省际差异大、未来会加速进入深度老龄化社会、城市人口老龄化问题更加严重以及“未富先老”等。人口老龄化会通过影响劳动力供给、社会资本形成以及技术创新三个方面影响经济发展。“十四五”期间积极应对人口老龄化,需要“放大”其对经济发展的正面效应,“缩小”负面效应,实行一揽子政策,从三个方面着手,分别是：增加全社会劳动力供给以及挖掘人口“二次红利”、通过不同渠道养老资金的统筹安排促进全社会资本形成以及通过拉动消费促进产业结构升级与技术创新。     

章太炎群独思想及其当代启示

与同时代的思想家不同,章太炎的群独思想蕴含着对现代性的反思与超越.在群独理论的建构中,他以互释的方式阐释群与独的概念,辩证地指出大独必群,群必以独成.在此,他解构了传统群独对立的二元模式,建构了群独融通合一的大独观与大群观.章太炎以个人主义为价值原则,但在不齐而齐的本体原则与各是其是的秩序理想的阐释下,价值原则最终导向了群体,体现出其群独思想中个体与群体同向性的理论旨归.在全球化视域下,在共生原则的支配下,章太炎的群独理论可为当今时代思考个体与共同体的关系提供有价值的思想资源.     

Creating an Ecological Field of Hope

JIANG Yijia has an ambitious goal in life.The successful entrepre-neur wants to bring hope to his fellow villagers by improving their life quality and beautifying their countryside.Jiang,who won a national award for his contribution to poverty allevia-tion,is the Party secretary of Gongshi Joint Village (consisting of Gongshi and five other neighboring villages) in Sui-ning City,Sichuan Province.Under his leadership,the village that had no road access and whose farmland was largely abandoned is now full of fruit trees on the mountain slopes,while the fields located at the foot of the mountain are fertile and lush.Most villagers now live in storied houses,drive their own cars,and have tap water and natural gas in their homes.In their spare time,villag-ers go to the village cultural and sports activity center to exercise,read books,and play chess.     

My Focus on Rural Revitalization

From the Report on the Work of the Government to the draft Outline of the 14th Five-Year Plan(2021-2025)for National Economic and Social Development and the Long-Range Objectives Through the Year 2035,rural revi-talizationhas virtually become a hot topic for this year's sessions of China's top legislature and top political advi-sory body.Deputies to the 13th National People's Congress(NPC)and members of the 13th National Committee of the Chinese People's Political Consultative Conference(CPPCC)handed in their pieces of advice and proposal that aim to comprehensively promote the country's rural revitalization.     

科学发展观与历史唯物主义关系探析

“以人为本,全面、协调、可持续”的发展观的提出,不是偶然的,它的形成有其历史必然性。它的形成既是在我国改革开放的伟大实践中,以社会主义经济快速发展为基点,以可持续发展为必经阶段,深刻总结国际国内经济社会发展经验教训的基础上形成的,更是以马克思主义理论尤其是历史唯物主义为其理论前提的。     

作为方法的华南:中心和周边的时空转换

"中心"和"周边"是两个灵活的、相对的概念,从儒家文化的视角着眼,"周边"并不限于中国的少数民族地区,还涵盖了包括东亚、东南亚等周边国家和地区.特别是在华南区域,"周边"的概念在汉族社会内部的不同民系、汉族和少数民族等各族群的互动中,又有着新的内涵和外延.从这一研究视角出发,我们可以重新审视以宗族为中心的华南汉族社会结构的研究、华南族群与互动的研究、华人和东南亚社会的人类学研究.在某种意义上,我们可以窥视华南视野下中国研究的特点.可以认为,华南研究已经具备了方法论的含义.     

Creating an Ecological Field of Hope

JIANG Yijia has an ambitious goal in life.The successful entrepre-neur wants to bring hope to his fellow villagers by improving their life quality and beautifying their countryside. Jiang,who won a national award for his contribution to poverty allevia-tion,is the Party secretary of Gongshi Joint Village (consisting of Gongshi and five other neighboring villages) in Sui-ning City,Sichuan Province.Under his leadership,the village that had no road access and whose farmland was largely abandoned is now full of fruit trees on the mountain slopes,while the fields located at the foot of the mountain are fertile and lush.Most villagers now live in storied houses,drive their own cars,and have tap water and natural gas in their homes.In their spare time,villag-ers go to the village cultural and sports activity center to exercise,read books,and play chess.