鸭疫里氏杆菌敏感烈性噬菌体的分离鉴定

为探索鸭疫里氏杆菌病的生物疗法,以富集法对健康番鸭的粪便进行了分离,获得1株鸭疫里氏杆菌敏感的烈性噬菌体,命名为RAP15.该噬菌体能裂解2株鸭疫里氏杆菌(RAf63和RAf71),说明噬菌谱较窄.透射电镜观察结果显示,该噬菌体由呈多面体的头部(直径约55 nm)和细长的尾部(长约200 nm)组成.RAP15的基因组可以被DNase Ⅰ消化,但不能被RNase A和绿豆核酸酶消化,提示其核酸类型为双股DNA.形态学和分子特征提示,RAP15属于长尾病毒科.     

表达Asia 1型口蹄疫病毒P1-2A和3C基因的重组山羊痘病毒弱毒株的构建

为构建表达Asia 1型口蹄疫病毒(FMDV)P1-2A和3C基因的重组山羊痘病毒(GTPV)弱毒株,将Asia 1型FMDV衣壳蛋白前体P1-2A基因、蛋白酶3C基因、绿色荧光蛋白(EGFP)基因、痘苗病毒启动子(p7.5)相连,构建成一整体基因表达盒p7.5-EGFP-P12A3C.以GTPV胸苷激酶基因序列中的Kpn Ⅰ酶切位点为插入位点.将整体基因表达盒插入转移载体骨架pUC119-TK中,构建GTPV转移载体pTK-p7.5-EGFP-P12A3C.将转移载体转染GTPV弱毒株AV 41感染的BHK-21细胞,转染后24 h就可见到绿色荧光,表明,成功获得了能表达目的蛋白的重组山羊痘病毒株TK-/EGFP+/FMDV P1-2A3C+/GTPV.     

俄国1905—1907年革命中的农民决议运动

俄国1905-1907年革命时期,农民决议运动这种独特的人民群众斗争形式获得广泛传播.农民决议和委托书包含了广泛的经济、政治和社会生活要求,涵盖了当时社会斗争的所有范畴,是俄国1905-1907年革命时期形成的农民纲领.农民制定、讨论和通过决议和委托书是一种重要的农民运动形式,对于农民的社会意识的发展起到了极大的推动作用.对于研究者来说,它们是折射20世纪初俄国农民精神面貌的一面镜子.     

用人单位单方调岗行为的法律效力研究

本文通过对用人单位单方调岗行为相关劳动争议的分析,在对我国现行相关法律法规梳理的基础上,提出了建立用人单位单方调岗行为规则、设置规范的调岗程序等完善我国现有法律法规和规制用人单位单方调岗权的建议.     

论行政执法与刑事诉讼的衔接

行政执法与刑事诉讼衔接是我国整顿和规范市场经济秩序、打击行政执法领域经济犯罪的必然选择,实践中行政执法与刑事诉讼衔接受立法不完善、行政自由裁量权滥用、监督不力等因素制约.借鉴国外行政刑罚制度、增设过失未移交刑事案件罪等手段是行政执法与刑事诉讼衔接的根本保证;规制行政执法自由裁量权,加强监督是关键因素;增强行政执法行为的独立性,保证检察机关依法独立行使职权是重要保障;检察机关增强法律监督能力、强化法律监督意识是重要依托.     

论合作型乡镇政府农村公共服务供给模式——基于江苏S镇社会管理创新考察

乡镇政府承担着极其繁重的公共服务责任,其公共服务质量的好坏直接关系到广大农民生活质量能否得到提高。在公共服务供给模式创新的过程中,合作型乡镇政府公共服务供给模式逐步成为一种趋势。     

The Influence of Subsidiary Directors from Headquarters and Equity Incentives on Technological Innovation from the Perspective of Subsidiary Board

To find out whether the purpose of subsidiary board is only to satisfy the local legal requirements, this article, based on the relevant data of listed firms in Shanghai and Shenzhen stock exchange in China, through empirical study, tries to show that: subsidiary directors belonging to enterprise group have a positive impact on innovation; directors' equity holdings have an inverted U-shaped impact on innovation; directors from enterprise group and director shareholding cooperative stimulate the innovation ability of subsidiaries. The impact of subsidiary board on innovation is through the intermediary effect of R&D. Our paper tests the impact of subsidiary board on innovation, and gives some suggestions on how the formation of a reasonable structure of subsidiary board.     

Intergenerational Transmission of Educational Aspirations in Chinese Families: Identifying Mediators and Moderators

Parents’ educational aspirations for youth play an important role in shaping youth’s own educational aspirations; however, little is known about how and in what context parents may transmit their aspirations to youth effectively. This is of particular interest and import to be examined in Chinese families, given Chinese cultural emphasis on educational achievement and Chinese youth’s outstanding academic performance internationally. By integrating several key theories of motivation and parental socialization (i.e., the expectancy-value model of academic achievement, the two-step model of value transmission, the contextual model of parenting, and the self-determination theory), the current study investigated simultaneously the mediating roles of parental involvement in youth’s learning and youth’s perceptions of parental aspirations, as well as the moderating role of parental warmth in the intergenerational transmission process of educational aspirations in Chinese families. A two-wave longitudinal study spanning about half a year was conducted among 323 Chinese seventh graders (54% female; M_age?=?13.25 years) and one of their parents (median educational attainment?=?completion of high school, median monthly income?=?USD 766–1226). It was found that parental educational aspirations for youth were related positively both indirectly through parental involvement and directly to youth’s perceptions of parental aspirations, which in turn were associated positively with youth’s own educational aspirations about half a year later. It was also found that parental educational aspirations for youth and youth’s own educational aspirations were associated positively with each other only when youth reported experiencing high levels of parental warmth, but unrelated when youth reported experiencing low levels of parental warmth, whereas such moderating effects of parental warmth were absent on the links from parental aspirations to youth’s perceptions of parental aspirations and parental involvement. These findings highlight the importance of integrating multiple theories to understand parent-to-youth transmission of educational aspirations in non-western cultures, which helps not only reveal generalizability, as well as boundary conditions for Western-originated theories, but also inform practical endeavors at promoting youth’s educational achievement worldwide to draw on strengths of different cultures.     

我国紧急状态法制与国际条约关系

我国紧急状态法制与国际条约的协调事实上不存在冲突的问题,其共同构成紧急状态法制克减公民权利的界限。此外,立法者可以在《戒严法》的基础上制定《紧急状态法》,并采用现代宪法对紧急状态法的立法原则。     

转型期中国社会劣势群体问题——析21世纪中国社会政治发展进程中的一道难题

中国社会正处于一个重大的转型时期。在这个转型期中出现了较为明显的社会不同阶层群体的分化。使社会劣势群体问题突现出来。面对社会劣势群体问题的严重化,我们必须加强对策思考并在实践中采取有效措施,才能化解21世纪中国社会政治发展进程中的这道难题。