Development of a heptaplex PCR system to analyse X-chromosome STR loci from five Italian population samples. A collaborative study

Many X-chromosome short tandem repeats (X-STRs) have been validated for forensic use even if further studies are needed on allele frequencies and mutation rates to evaluate the extent of polymorphism in different populations and to establish reference databases useful for forensic applications and for anthropological studies. A single multiplex reaction of seven X-STRs, which includes the DXS6789, HUMARA, DXS10011, DXS7423, HPRTB, DXS6807, DXS101 loci, is presented and their allele frequency distribution in a large population sample including 556 subjects (268 females and 288 males) analysed by five forensic laboratories of Central and Northern Italy is shown. Our results demonstrate the feasibility of a single amplification/detection reaction involving seven markers of the X chromosome, which can be fruitfully used in complex kinship analysis.     

A Y-chromosome STR marker should be added to commercial multiplex STR kits

Abstract:  Autosomal short tandem repeat (STR) analysis has become highly relevant in the identification of victims from mass disasters and terrorist attacks. In such events, gender misidentification can be of grave consequences, yet the list reporting amelogenin amplification failure using STR multiplex kits continues to grow. Presented here are three such examples. In the first case, we present two male suspects who demonstrated amelogenin Y-deficient results using two commercial kit procedures. The presence of their Y chromosomes was proven by obtaining a Y-haplotype. The second case demonstrated a profile from a third male suspect where only the Y homolog of the XY pair was amplified. In events such as mass disasters or terrorist attacks, timely and reliable high throughput DNA typing results are essential. As the number of reported cases of amplification failure at the amelogenin gene continues to grow, we suggest that the incorporation of a better gender identification tool in commercial kits is crucial.     

Human height estimation from highly distorted surveillance image

Video surveillance camera (VSC) is an important source of information during investigations especially if used as a tool for the extraction of verified and reliable forensic measurements. In this study, some aspects of human height extraction from VSC video frames are analyzed with the aim of identifying and mitigating error sources that can strongly affect the measurement. More specifically, those introduced by lens distortion are present in wide-field-of-view lens such as VSCs. A weak model, which is not able to properly describe and correct the lens distortion, could introduce systematic errors. This study focuses on the aspect of camera calibration to verify human height extraction by Amped FIVE software, which is adopted by the Forensic science laboratories of Carabinieri Force (RaCIS), Italy. A stable and reliable approach of camera calibration is needed since investigators have to deal with different cameras while inspecting the crime scene. The performance of the software in correcting distorted images is compared with a technique of single view self-calibration. Both approaches were applied to several frames acquired by a fish-eye camera and then measuring the height of five different people. Moreover, two actual cases, both characterized by common low-resolution and distorted images, were also analyzed. The height of four known persons was measured and used as reference value for validation. Results show no significant difference between the two calibration approaches working with fish-eye camera in test field, while evidence of differences was found in the measurement on the actual cases.     

Perspectives,practices and prospects of public affairs in Central and Eastern Europe: a lobbying future anchored in an institutional context

‘Public Affairs’ is not a term most people in Central and Eastern Europe (CEE) will recognise and associate with interest representation and lobbying as ways of linking business, society and government. The term may be seen as a euphemism for corruption-inspired ‘lobbying’, an artificial and confusing umbrella term or a public relations creation. It is a term in transition for a subject in transition in a part of Europe in transition. Even as part of the European Union, CEE is still different, in transition, finding its own feet like a phoenix arising from the ashes of 50 years of communism, but slowly, in bursts, réculer pour mieux sauter and very different from country to country. Which are the features and values of the 50 years of communism that will disappear, fast more slowly, which ones will linger on? What are the institutions in CEE countries that public affairs will have to deal with, and what is the view on transparency and transparency regulation in CEE? To add understanding, values, cultural differences, institutional context and other factors affecting the public affairs environment are examined. Copyright © 2014 John Wiley & Sons, Ltd.     

Europe as a Beacon of Democracy? Citizenship Policies Relating to Youth and Migrants in Portugal

Abstract

This article aims to compare discourses about national and European policies on active citizenship and democratic participation, with a particular focus on youth and migrants. For this purpose we analysed official documents of public institutions and nongovernmental organizations (NGOs) in order to assess how the process of Europeanization has influenced national policies with regard to increasing political participation and citizens' civic awareness. Additionally, we conducted interviews with policy makers and NGO leaders in order to integrate and compare different levels of discourse and thus identify potential dissonances. Analysis of the documents shows that there is a strong concern to match national policy priorities with those established by international organizations. Notwithstanding positive perceptions, NGO leaders and policy makers criticize the ways policies have been implemented, stressing the need to adopt a strategy that bridges the gap between the prescribed and the real, as well as the importance of overcoming the hegemony of economic factors in policy decisions. In this regard, NGO leaders criticize the cynicism of political leaders and policies motivated by demographic and economic concerns. In relation to European identity and integration, NGO leaders argue that Europe must be collectively constructed; yet, policy makers stress that the failure of the Constitutional Treaty in 2005 resulted from a deficit in the negotiation process. In sum, this article suggests that it is necessary to promote greater involvement of civil society in the design and implementation of policies which, in turn, may contribute to the strengthening of shared democratic principles.     

Long Allele Designations at D2S1338 and D19S433 Loci as Influenced by Various Multiplex STR Kits

European forensic laboratories are replacing the STR multiplex kits with the new generation 16/17 STR kits. This study examines the influence of the new generation kits and the new Applied Biosystems 3500xL Genetic Analyzer on the designation of long D2S1338 and D19S433 off‐ladder alleles. Different allele calls were obtained using the new NGM? (Applied Biosystems) and PowerPlex^® ESI? (Promega) kits compared with AmpF?STR^® SGM Plus? kit (Applied Biosystems). Sequence analysis was used to determine accurate allele designation. The new multiplex kits and the 3500xL Genetic Analyzer improved accuracy of long allele designations. DNA databases worldwide include countless profiles obtained by previous kits. Discrepancies between the new and former technologies may cause failure to detect hits. Discordance is expected due to primer sequence differences between various kits. An additional discordance, occurring in long alleles, independent of primer sequence is reported in this study.