Use of Embryos Extracted from Individual Cannabis sativa Seeds for Genetic Studies and Forensic Applications

Legal limits on the psychoactive tetrahydrocannabinol (THC) content in Cannabis sativa plants have complicated genetic and forensic studies in this species. However, Cannabis seeds present very low THC levels. We developed a method for embryo extraction from seeds and an improved protocol for DNA extraction and tested this method in four hemp and six marijuana varieties. This embryo extraction method enabled the recovery of diploid embryos from individual seeds. An improved DNA extraction protocol (CTAB3) was used to obtain DNA from individual embryos at a concentration and quality similar to DNA extracted from leaves. DNA extracted from embryos was used for SSR molecular characterization in individuals from the 10 varieties. A unique molecular profile for each individual was obtained, and a clear differentiation between hemp and marijuana varieties was observed. The combined embryo extraction–DNA extraction methodology and the new highly polymorphic SSR markers facilitate genetic and forensic studies in Cannabis.     

Forensic databases,a perspective from the penitentiary centers of Spain

Scientific and technological progress in the field of forensic genetics is very useful in the resolution of criminal cases, but it entails the need for a deep ethical reflection, as the individual Fundamental Rights may be violated.This project aims to collect and compare the opinion of prisoners and prison officials on what characteristics the country's forensic database should have. In this context, 210 subjects were surveyed, 101 of them prisoners and the rest prison officials, from three different Spanish penitentiary centers.Among the results obtained, most prisoners and officials consider the national DNA database to be useful, and additionally, a 40% of the participants would support the integration of the profiles of the entire population. 64% considered it ethical to use the DNA profiles of the database as a tool for familial searching. Despite this, half of the respondents are concerned about the future uses of the DNA database.Integrating the opinion of these analyzed groups with other relevant judicial, scientific and ethical convictions, ensures the regulation between security and individual’s Human Rights.     

Towards Performance,Quality and Environmental Management in Local Government: the Case of Spain

The search for improvements in the management and quality of public services seems to be a constant in all public administrations, and there is an ever-increasing use of management techniques and models from the business sector. The aim of this paper is to show which management systems are being used by Spanish local governments to improve their management in terms of economy, quality and the environment. Our analysis is based on a questionnaire sent to Spanish local governments about the tools they use and the factors that influence their implementation. The results show that more efforts are necessary to implement performance measurement and total quality management, but they also show some positive aspects, such as the usefulness of performance measurement and its application to decisions in those local governments that have implemented it.     

A brief measure of the triarchic model of psychopathy among community youths: psychometrics and measurement invariance

ABSTRACT

The development and validation of empirically sound measures that operationalize the Triarchic model of psychopathy among youth is currently in the nascent stage. The present study aimed to examine the psychometric properties of a brief refined measure of the Triarchic model of psychopathy based on the Youth Psychopathic Traits Inventory (YPI-Tri-S) among a mixed-gender school sample of 782 youth (M?=?15.87 years, SD?=?1.72) from Portugal. Confirmatory factor analysis offered support for the theoretically expected three-factor structure. Cross-gender measurement invariance using the present sample and cross-sample measurement invariance of the YPI-Tri-S using a previously collected forensic sample of institutionalized males was established. The YPI-Tri-S showed adequate psychometric properties in terms of discriminant validity, convergent validity, concurrent criterion-related validity, known-groups validity, and internal consistency. Findings indicate that YPI-Tri-S is a valuable brief and psychometrically reliable and valid self-report measure of the Triarchic model of psychopathy for use in diverse samples of youth.     

The globaliser dragon: how is China changing economic globalisation?

There are many studies on the effects of both economic globalisation and the rise of China. These core issues of the contemporary international agenda entail major economic, military, environmental, social and cultural transformations in most nations. While there is also an abundant literature on how globalisation supported the rise of China, there are scarce publications on how China became one of the primary drivers of globalisation. This article assumes that understanding the power of globalisation over countries is as crucial as assessing the power of certain countries over the process. In this sense, it uses the recently created ‘theory of globalisers’ to analyse how is China transforming contemporary economic globalisation. The conclusion is that China became an ‘economic globaliser’ in the twenty-first century. As the largest exporter, the second largest importer, the third largest provider of foreign direct investments, and a major supplier of high-tech goods, the Asian giant is a vital partner for several economies in different continents. In this context, Beijing’s globalisation strategy aims at both securing the benefits of globalisation and reforming the international economic order, but without a revolutionary stance.     

Who wins the most when everybody wins? Predicting candidate performance in an authoritarian election

ABSTRACT

What explains the variation in vote shares received by candidates in single-party authoritarian elections where everybody wins? The scholarly literature has often ignored institutional variations, treated all authoritarian elections as similar, and explained the variation of vote shares as a consequence of clientelism, coercion or electoral fraud. We employ a unique data set for Cuba’s 2013 National Assembly election to show an alternative answer: even in authoritarian regimes, institutional settings shape voters’ behaviour and candidates’ strategies. When the number of candidates on the ballot equals the number of parliamentary seats and yet voters can express some preference among multiple candidates, valence can become a predictor of candidate performance. Voters reward high-quality politicians, but not incumbents or Communist Party members, while candidates have no incentives to actively distinguish themselves and converge toward the general support of the single united slate.     

ORGANIZING FOR EU IMPLEMENTATION: THE EUROPEANIZATION OF GOVERNMENT MINISTRIES IN ESTONIA,POLAND, AND SLOVENIA

Do different levels of exposure to EU law implementation have consequences for the organization of national ministries? Previous accounts suggest that European integration has little influence on the ‘hardware’ of member state administrations. Data covering the organization of 21 ministries in Estonia, Poland, and Slovenia show the Europeanization effect to be more pronounced than might be expected. Ministries responsible for transposing many EU directives tend to institutionalize centralized oversight in legislative planning, review, and monitoring; ministries with few implementation responsibilities rarely make such adaptations. This effect holds when one controls for the impact of partisan preferences and organizational conventions. The results offer a counterpoint to the studies of old member states that find little EU effect on the organization of domestic ministries.     

“The big sleep: Elucidating the sequence of events in the first hours of death to determine the postmortem interval”

Recent developments on postmortem interval estimation (PMI) take an advantage of the autolysis process, pointing out to the analysis of the expression of apoptosis and autophagy genes towards this purpose. Oxidative stress plays a role in this signaling as a regulatory mechanism and/or as a consequence of cell death. Additionally, melatonin has been implicated on apoptosis and autophagy signaling, making melatonin a suitable target for PMI determination. The aim of this study was to investigate the early PMI through the analysis of the expression of autophagy genes as well as oxidative stress and melatonin receptor. Our results demonstrated a rapidly increased on the expression of autophagy genes according to the expected sequence of events, then a marked decrease in this expression, matched with the switch to the apoptosis signaling. These results revealed potential candidates to analyze the PMI in the first hours of death, helping to estimate the time-since-death.     

A River,a Dam and A Hawthorn Tree

The Three Gorges area on the middle reaches of the Yangtze River is undoubtedly one of the most beautiful and exciting places in China,and the perfect destination to admire China's natural and manmade marvels.In May,I followed my inner siren call and visited the area on a sunny weekend with my boyfriend.We took a cruise on the Yangtze.The ship was swarming with domestic tourists and several foreigners,many of us leaning over the railings in awe.I had booked seats on the top deck,and we were not disappointed as the panorama of the Xiling Gorge passed us by along with the lush vegetation on the hills and teal waters of the Yangtze.