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H C Lee E M Pagliaro K M Berka N L Folk D T Anderson G Ruano T P Keith P Phipps G L Herrin D D Garner 《Journal of forensic sciences》1991,36(2):320-330
Deoxyribonucleic acid (DNA) was isolated from a number of spongy and compact human bone tissue specimens, and the yield was estimated on a "per milligram of starting tissue" basis. DNA was, in addition, isolated from a number of corresponding blood and bone tissue specimens. Spectrophotofluorometry and ethidium bromide visualization on minigels were used to estimate the quantity and degree of degradation of DNA. The DNA from several blood-bone pairs is shown to give concordant restriction fragment length polymorphism (RFLP) typing results by two different typing protocols with five different single-locus probes. DNA from several additional blood-bone pairs is shown to give concordant results for human leucocyte antigen (HLA)-DQ alpha phenotypes following polymerase chain reaction (PCR) amplification and hybridization to specific allele-specific oligonucleotide (ASO) probes, and for the variable numbers of tandem repeats (VNTR) length polymorphisms 3' to the human apolipoprotein B (APOB) gene following PCR amplification with specific primers and analysis of the products by electrophoresis and ethidium bromide visualization. 相似文献
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Donna Lee Van Cott 《拉美政治与社会》2003,45(2):1-39
The central question of this article is why indigenous social movements formed electorally viable political parties in Latin America in the 1990s. This development represents a new phenomenon in Latin America, where ethnic parties have been both rare and unpopular among voters. Institutional reforms in six South American countries are examined to see if the creation and success of these parties can be correlated with changes in electoral systems, political party registration requirements, or the administrative structure of the state. The study concludes that institutional change is likely to be a necessary but not sufficient condition for the emergence and electoral viability of ethnic parties. 相似文献
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This article analyzes demographic change in Bremen 1815–1914 against the background of changing employment opportunities. Within the pre-industrial employment structure, which still prevailed in the decades after 1850, in-migrants were particularly disadvantaged. When modern industries developed, the situation changed. At the beginning of the twentieth century both male and female in-migrants benefited from a higher life expectancy than the city-born population had. The strong sectoral changes in employment opportunities in Bremen at the turn of the century reinforced the trends towards family limitation. More skilled in-migrants felt the need to adapt their reproductive behavior to facilitate upward social mobility. The article argues for a wider use of archival data, like those used in their study, to explore issues relating to urban demographic change in German cities during industrialization. 相似文献