猪传染性胃肠炎病毒S基因抗原位点B与C之间片段的克隆与原核表达

将RT-PCR扩增的猪传染性胃肠炎病毒S基因抗原位点B与C之间的目的片段经克隆、双酶切后连接于表达载体,经酶切、PCR和测序鉴定的阳性重组质粒转化BL21(DE3),用IPTG诱导重组菌株表达其融合蛋白.结果显示,目的片段的大小为357bp,序列分析表明,S基因与其他猪传染性胃肠炎病毒相应基因具有很高的同源性;Western-blotting检测表明,分子质量约34ku的融合蛋白具有良好的反应原性.     

Peasants and labourers in Southern Spain,Cuba, and Highland Peru

This paper discusses some of the differences and similarities between a peasantry and an agricultural proletariat and considers the economic relations between peasants or labourers and landowners. The examples are drawn mainly from studies of Southern Spain, Cuba and Peru. The paper de‐emphasises the specificity of ‘peasant society’ as an object of study both from the economic and the socio‐political point of view.     

Top-Level Design for Chinese-Style Public Diplomacy against the Backdrop of Globalized Communication

<正>In the era of new media, the nature of being real-time and globalized of the public communication has posed unprecedented challenges to China’s public diplomacy as a whole. This is mainly manifested in the increasing obscurity of the boundary between domestic and foreign affairs. Any domestic event,     

From bureaucratic centralism to self‐regulation: The reform of higher education in Spain

Theories of self‐regulation in higher education claim that autonomous institutions are better able to fulfil their educational and scientific goals. The reforms initiated in Spain in thel970s and which finally crystallised in the Ley de Reforma Universitaria (University Reform Act) in 1983 have taken this path, granting autonomy to the universities and acknowledging that the policy network has been enlarged through the incorporation of new actors (students, junior lecturers, regional political elites), and that this is no longer the exclusive domain of the university professors. However, this article argues that the reforms have not had the expected results insofar as quality is concerned: deregulation has been introduced without establishing the conditions for the institutions to become fully responsible for their decisions, so encouraging the persistence of self‐interested and often anti‐scientific attitudes among many academics.