人肺癌中TNF-α基因多态性分布的意义及其对细胞凋亡的影响

目的:观察TNF-α基因启动子区-308位核苷酸多态性基因型在肺癌和对照人群中的分布,以及这种多态性对该基因表达和细胞凋亡的影响。方法:PER-RFLP分析112例肺癌和99例正常人的TNF-α基因启动子区-308位核苷酸多态性,TNF-α-308*G等位基因记做TNF-α1,TNF-α-308*A等位基因记做TNF- α2。免疫组织化学方法检测肺癌组织中TNF-α的表达,比较不同基因型肺癌病例的标本之间TNF-α基因表达的差异性。TUNEL法检测肺癌组织中细胞凋亡,并对比分析TNF-α-308位核苷酸多态性及其对肺癌组织中TNF -α表达和不同类型细胞凋亡的影响。结果:肺癌组与对照组的基因型分布为TNF-α1/1(52．7％、46．5％)。 TNF-α1/2(35．7％、48．5％),TNF-α2／2(11．6％、5．1％);等位基因频率为TNF-α1(70．5％、70．7％),TNF- α2(29．5％、29．3％),组间没有显著性差异,P>0．05。两种基因型个体在TNF-α基因表达上,TNF-α2/2基因型个体高于TNF-α1／1基因型的个体,两者差异显著,P<0．01。TNF-α表达的高低与癌细胞凋亡有比较明确的关系,TNF-α表达高的病例,肺癌细胞凋亡也多,与表达低的病例之间有明显差异,P<0．01。TNF-α表达的高低与间质细胞凋亡之间没有明确的关系。间质细胞平均凋亡指数在高、低分化的肺癌中分别为3．4±0．4和 3．1±0．5,组间没有显著性差异,P>0．015;癌细胞的平均凋亡指数在高、低分化的肺癌中分别为2．4±0．3和 1．7±0．4,两组间有显著性差异,P<0．05,,结论:TNF-α-308位核苷酸的多态性基因型在肺癌和正常人中的分布之间没有明显差别,这种多态性可能不是肺癌易感的遗传因素。但这种多态性与该基因的表达有关。TNF-α高表达肺癌细胞凋亡较多,间质细胞凋亡与TNF-α表达的高低变化之间没有明确的关系。在高分化的肺癌中, TNF-α表达较高,癌细胞凋亡也多,TNF-α-308位核苷酸的多态性可能与了肺癌的分化有关。     

第一次数学危机及其哲学分析

毕达哥拉斯关于数的信条及以数为基础的宇宙模型的破产,导致了第一次数学危机。这一危机的影响是巨大的,它不仅推动了数学及其相关学科的发展,使古希腊数学的基础发生了根本性的变化,而且推动了整个科学的发展。在古希腊,数学和哲学是结盟的,哲学使古希腊的数学趋于抽象和真理。正是由于古希腊的哲学背景,使其有可能建立世界上第一个数学公理系统,并最终导致了近代科学的诞生。     

公务员培训需求分析方略

《公务员法》的颁布使公务员培训工作纳入了法制化的轨道。高效率的公务员培训建立在对培训需求科学分析基础之上。培训需求循环分析模型、培训需求任务-绩效分析模型和YGWB培训需求分析模型,在多年公务员培训实践中被证明是行之有效的培训需求分析方法。对这些方法的研究和总结既有理论上的价值也有实践上的意义。     

我国宪法中刑事诉讼制度的规范分析

宪法与刑事诉讼法都涉及国家权力与公民基本权利的关系问题,因而刑事诉讼与宪法的关系相当紧密。其中基于宪法国家根本法的地位,刑事诉讼基本制度和原则都可以在宪法规范中找到根据。我国现行宪法为刑事诉讼制度提供了最高效力的法律支撑,宪法是刑事诉讼制度运行的基础。同时也应当看到宪法所提供的支撑还不充足,有继续加以完善的必要。     

Security in the name of human rights: the discursive legitimation strategies of the war on terror in France

This article aims to question the discourse on “the war on terror” developed by the French President in the wake of the two terrorist attacks that occurred in France in 2015. Drawing from critical discourse analysis, it explores the discursive legitimation strategies deployed by President Hollande to legitimate France’s securitarian response to the two attacks. It reveals how the defence of human rights served as an overall justificatory framework, through rationalisation, appeals to authority, and moralisation. It argues that Hollande implemented a discursive manipulation of reality to shield his actions from criticisms of illiberalism, all the while reframing the notion of human rights, undermining it and paving the way for an authoritarian-driven executive.     

Learning-Shaping Crises: A Longitudinal Comparison of Public Personnel Reforms in Italy, 1992‒2014

Abstract

This article analyses the attempts to reform public administration, notably personnel management, in Italy between 1992 and 2014, with a focus on implementation and the period following the multiple crises that have unfolded since 2008. By untangling the policy learning processes between multiple crises, past reform attempts and domestic and European “contexts in motion”, the article finds that efficiency-oriented reforms have floundered regardless of the political color of governments or indeed of the nature – political or technocratic – of the governments. Domestic factors, notably the frequency of government alternation, i.e. government instability, and European pressure have further reinforced the orientation towards single-loop lessons, i.e. the almost exclusive effecting of short-term cost-cutting measures.     

Passport to Parenthood: Reproductive Pathways In and Out of Denmark

Denmark has become a destination for single women, lesbians, and heterosexual couples wanting donated sperm. At the moment, women from Sweden, Norway, Germany, Italy, and the UK travel to Denmark. Simultaneously, waiting lists for donated eggs and age restrictions are prime motivations for infertile Danish women and heterosexual couples to leave Denmark and travel to Spain, the Czech Republic, the Ukraine, and Greece for egg donation. Informed by Donna Haraway’s notion of “the apparatus of bodily production”, Marcia Inhorn’s development of “reproductive flows”, and the use of Adele Clarke’s “situational analysis”, this paper explores the question: How do global reproductive pathways in and out of Denmark emerge when fertility travellers narrate, negotiate, and cross national borders to go through fertility treatment? Methodologically, we use a multi-sited and multi-modal approach centring on interviews with fertility travellers moving to and from Denmark in combination with ethnographic observations carried out in Danish and Spanish fertility clinics and an analysis of legal regulations. The paper concludes by discussing how the concept of reproductive pathways helps to theorize transnational movements of bodies and contributes to feminist scholarship on transnational reproductive travel.     

在校女大学生的择业倾向及其影响因素——基于北京市大学生的调查

用问卷调查法对北京市30多所高校大学生就其择业倾向的调查结果显示:大部分女大学生把工作稳定排在首位;首选的就业地区是经济发达的大中城市;大部分认为工作与所学专业要有一定关联;期望的月薪集中在2500~5000元之间,工作的首要目的是实现自我价值;低收入家庭选择找工作的比例高,高收入家庭选择准备出国的比例高;愿到基层就业的女大学生总体趋势随着家庭年收入的增加而下降;高收入家庭的学生更看重第一份工作对自我成长的价值,而低收入家庭的相对看重先自食其力;成绩排名靠前的学生选择继续深造的比例高,成绩排名靠后的选择找工作的比例高。     

中国大气保护公共支出的绩效评价——基于各地区环境数据的比较分析

对我国各省的大气环保支出的绩效进行评价表明,大气公共支出绩效的提高与地方环保机关的工作水平和工作效率紧密相关。在客观条件一定的情况下,地方环保部门是能够通过自身效率的提高来促进大气环境的改善。     

17例杀亲案件的回顾性分析

目的总结杀亲案件的特点,为此类案件的分析、预防提供参考。方法调查溧阳地区2004—2014年破获的17例杀亲案件,对原始登记信息、现场勘验笔录、尸体检验鉴定书、破案情况进行统计分析。结果 17例杀亲案件中受害人以女性居多,嫌疑人以男性居多,杀人动机以配偶不忠或怀疑配偶不忠占较高比例。杀亲案件中有精神病患者杀人、伪装杀人,嫌疑人杀人后自杀占有一定比例。结论杀亲案件多与婚外性行为、精神病患者杀人等家庭因素有关,部分为杀人旋即自杀,致伤工具具有简单随意、易于获取的特点。