应用免疫磁珠分离混合斑中精子

目的探讨应用免疫磁珠法分离精子-上皮细胞混合液中精子的可行性。方法制备不同比例的精子-上皮细胞混合液各15例,分别应用免疫磁珠法及差异裂解法提取精子,经PCR扩增进行DNA-STR分型,比较两者的差异。结果免疫磁珠可以特异性捕获精子,对精子个数为500～2 000/mL的混合斑STR分型成功率(正确分型13个以上,RFU200)为93.33%,差异裂解法为46.67%,两种方法存在明显差异(P0.05)。结论免疫磁珠可有效分离精子和混合斑中其他成分,在精子个数低于2 000个/mL检材中优于差异裂解法。     

The Timing of Split-Ticket Voting Decisions in Mixed Systems: Evidence from South Korea

Abstract

Although two-vote mixed legislative systems have proliferated globally, the factors contributing to split-ticketing in these cases remains poorly understood. Using survey data regarding South Korea's 2008 National Assembly election, this article addresses two questions: Is ticket-splitting in two-vote legislative systems influenced by the timing of one's vote decision and are late deciders more or less rational in their decision to ticket-split than early deciders? Empirical analysis finds that split-ticket voting under various specifications is more likely to be carried out by late deciders. Among split-ticket voters, however, late deciders are more likely to irrationally split their vote.     

Valence politics and economic crisis: Electoral choice in Canada 2008

This paper investigates factors affecting voting behavior in Canada’s October 2008 federal election. The election was held in the context of a rapidly worsening financial crisis that threatened to become a global economic meltdown. National survey data gathered in the 2008 Political Support in Canada Study reveal that the deteriorating economy trumped the opposition Liberal Party’s Green Shift Program as the major campaign issue by a huge margin. Damage done to the governing Conservatives by the economic crisis was limited by perceptions of their leader, Prime Minister Stephen Harper. Analyses of a mixed logit model of electoral choice shows that although the Conservatives had a relatively small share of party identifiers and Harper was widely disliked, his image as “safe pair on hands” helped his party weather the political storm generated by the flood of bad economic news.     

Staying sincere: an experimental analysis of non-strategic voting in South Korea

Under ideal conditions, when do voters switch from sincere to strategic voters? Despite a large literature on strategic voting and Duverger’s Law, few studies assess when individuals shift from sincere to strategic nor what factors may encourage or discourage defecting from one’s first choice. Through an experimental web survey analysing district voting in South Korea’s National Assembly, this study measures the effect of partisan cues and the margin of error. Not only did a non-negligible number of respondents remain sincere voters despite conditions favourable to strategic voting, but supporters of smaller parties and those without partisan identification were more likely to defect from their sincere vote when given an additional prompt about the margin of error. The results suggest the contextual influence on the strategic vote calculus.     

Barriers to Integrating New Urbanism in Mixed-Income Housing Plans in Chicago: Developer,Housing Official,and Consultant Perspectives

This article explores the barriers to implementing mixed-income development plans that incorporate the social (income) and physical (new urbanism) mixing goals of HOPE VI. I examine a comparative case study of three HOPE VI planning efforts in Chicago, Illinois, that exhibit different results. I draw from 25 in-depth interviews across three primary types of actors involved in the development process: developers, housing officials, and consultants. This research uses the perspectives of these key actors to identify the barriers that constrain the implementation of new urbanist designs. Current research indicates that mixed-income developments vary in their degree of income mixing and how new urbanist strategies are implemented. However, there is little consensus on why this is so. Findings indicate implementation of new urbanism is constrained by limited interagency coordination, restrictive design policies, low community buy-in, and exclusive marketing and occupancy practices. Overall, the research offers lessons learned from which I recommend changes in planning practices to assist actors in the consistent implementation of new urbanism in mixed-income developments.     

精斑检验干扰因素的研究

目的研究精斑检验中预试验与确证试验结果的关系,以及取材时间、生活习惯对确证检验结果的干扰。方法取376例阴道拭子,用酸性磷酸酶(ACP)染色法、斑点ELISA法和抗人精PSA金标试纸法检测检测精斑。结果ACP阴性时,可检出P30、精子,性生活后精斑确证检验大部分阳性检出分布在48小时以内样本中,结果经X2检验,P<0.01,差异性显著,而不同的生活习惯对P30检测具有显著性差异。结论研究结果对精斑的检验以及检材的提取具有推广应用和指导性意义。     

浅析混业经营对我国金融监管体制的影响

我国目前实行的金融监管体制是分业经营、分业监管。由于经济环境的变化,这种监管体制面临诸多挑战,主要是金融开放政策、金融创新、金融整合发展的挑战。通过借鉴国际经验,可以发展完善我国的金融监管。     

刑事诉讼实践中的若干问题研究

刑事诉讼实践中存在着一些问题,如法院将作出对被告人有利的无罪判决时,检察院以“事实、证据有变化为由”,申请撤诉,从而严重侵害了被告人的人权；无罪案件中的非法证据问题；法院是否可以变更起诉罪名和事实宣告被告人无罪；法院在宣告被告人无罪前,检察院申请撤回起诉等等。研究这些问题,对促进和完善刑事诉讼程序具有一定的实践和理论意义。     

塑料客体上蛋白质中介物手印的银染显现研究

对塑料制品上的鸡蛋清形成的蛋白质潜在手印进行硝酸银染色实验:在几种塑料制品上用鸡蛋清捺印样本,样本分别经无水乙醇固定、硝酸银-乙酸溶液染色、蒸馏水清洗三遍,晾干;再置于日光下光照至鸡蛋清手印显出棕黄色清晰纹线,10天以内的手印均可显出。该方法操作简单,对于塑料制品上蛋白质潜在手印的显现效果良好,可以在实践中加以应用。     

国际组织的法律责任——角色定位与法律基础分析

国际组织的人格局限于基本文件规定的范围和条件之下，其只可能在这个有限主体范围内从事活动，因而只在此范围内对其国际行为直接承担责任。国际组织虽然只具有派生的权利能力和行为能力，但它是以自己的主体资格承担国际法律责任的。混合条约应当明确规定国际组织及其成员国各自的权责界限，这是解决混合条约情形下的权责分配问题的有效办法。