抑制消减杂交技术筛选大鼠急性心肌缺血后差异基因

目的采用抑制消减杂交技术,筛选早期心肌缺血关的RNA分子,为相火研究和应用提供依据。方法10只SD雄性大鼠结扎冠状动脉前降支1h,制作心肌缺血模型。以缺m心肌的RNA分了为实验组,以非缺血区Ⅸ的RNA分子为对照组,朋Chmtech公司抑制消减杂交试剂盒进行杂交。经蓝白斑筛选阳性克隆测序斤经过斑点杂交确认,构建早期大鼠心肌缺血后抑制消减杂交文库,所得序列和Genbank数据库比对。结果筛选到10个阳性克隆和11个未知基因的表达序列标签（expressedsequencetag,EST）,按照功能分类,包括代谢相关的酶类基侧,核糖体和翻译相关的基斟,心肌重塑相芙的基旧等,其叶1组织蛋白酶LI,basi面n等基因等是首次报道与早期心肌缺血相芙。结论采用抑制消减杂交技术获得的差别表达基因可为早期心肌缺血提供新的目标分子。     

进一步推动GMS合作升级 共建21世纪海上丝绸之路

共建21世纪海上丝绸之路与大湄公河次区域(GMS)国家有直接联系。20多年来,GMS国家已经在多个合作机制中取得了进展。本文就推动GMS合作升级,共建21世纪海上丝绸之路提出了建议。     

参建海上丝绸路 打造海陆新门户——广西北部湾经济区参与共建21世纪海上丝绸之路探讨

本文分析广西北部湾经济区参与共建21世纪海上丝绸之路的优势及基础,提出广西北部湾经济区参与共建21世纪海上丝绸之路、加快与东盟合作发展的建议。     

中国国家荣誉制度立法的历史考察( 1881 -1949)

晚清政府在借鉴西方勋章制度的基础上,颁发了《奏定宝星章程》,开了近代中国国家荣誉立法之先河.其后的民国政府颁发了《勋章章程》、《颁给勋章条例》等系列规范,构建了以勋章制度为基本内核的国家荣誉制度.早期中共政权也制定了英模表彰等方面的条例和规范.中国近代以来诸如此类的国家荣誉立法例,为新中国的国家荣誉制度立法和建设提供了历史借鉴和启示.     

蒙巴顿对战后初期缅甸政局的影响

二战末期,盟军东南亚战区总司令蒙巴顿在负责缅甸军管时期力排众议,坚决与缅甸民族主义力量实施合作,并吸纳缅甸本土武装加入正规军。缅甸文官政府回归后,蒙巴顿继续关注缅甸事务,举荐新任缅甸总督,并积极推动英国工党政府调整对缅政策。蒙巴顿的主张,顺应了民族自决和非殖民化的国际趋势,对战后初期的缅甸政局、英缅关系和缅甸独立进程产生了深远影响。     

Early Voting and Campaign News Coverage

The proportion of votes cast before election day has risen steadily over the last two decades. Previous research asked how early voting has impacted voter participation. In this article, we ask how early voting has affected the flow of information to voters through the mass media. By increasing the number of days voters are able to vote, are we also increasing the number of days that candidates and campaigns continuously disseminate campaign-related information to the news media? Is news coverage of campaigns quantitatively and qualitatively different when opportunities to vote early are available and utilized? Our expectation is that early voting significantly influences the volume and nature of campaign news coverage. We study the effects of early voting on campaign news coverage of gubernatorial and Senate races in 2006 and 2008. Our findings reveal that the volume and content of campaign news coverage is significantly influenced by early voting.

[Supplementary material is available for this article. Go to the publisher's online edition of Political Communication for the following free supplemental resource: Appendix for Early Voting and Campaign News Coverage—Alternative Model Specifications.]     

Poor relief and families in nineteenth-century Ireland and Italy

This article examines the extent to which poor relief supported families and households in two contrasting European (and predominantly Roman Catholic) countries, Ireland and Italy, over the long nineteenth century. The main focus of the article is on the poor law in Ireland and the extent to which it provided support to families and households. While nuclear and extended families dominated household structures in late nineteenth-century Ireland (at least in rural areas), we show that by 1900, the majority of persons supported in workhouses were single. However, outdoor relief also formed an important part of the Irish poor law system and data on the household composition of persons supported is not available from official sources. Drawing on a data-matching exercise for one poor law union, this article suggests that the household structure of persons on outdoor relief may have been more complex than the official data would indicate and, in contrast to indoor relief, much more representative of overall household structures. In order to put these findings in context we compare the Irish approach with that adopted in Italy.     

Minors,gender, and family: The discourses in the court system of traditional Buenos Aires

The article deals with public attitudes toward family responsibility in early-nineteenth-century Bienos Aires, particularly as revealed in lawsuits occasioned by disputes over the custody of minors. The care and education of minors was ordinarily entrusted to parents, but death, incapacity, licentious conduct, poverty or abandonment often resulted in the minor being removed from parents and taken by others into custody of guardians or institutions of public welfare. The majority of the analyzed lawsuits resulted from an attempt by parents to recover their authority over their children. The lawsuits indicate that the racial, gender, social, and generational “disadvantages” of plaintiffs did not a priori determine the judgement, but rather that arguments for family stability frequently were decisive when heard by sympathetic judges. Disadvantages persons thus frequently argued sucessfully about the meaning of the family, as protagonists in the legal system that otherwise often marginalized them.     

‘Till Death Us Do Part’: spousal homicide in early modern Russia

The history of domestic violence, let alone domestic homicide, in Russia has yet to be written. This article focuses on the legal attitudes to domestic and especially marital homicide in early modern Russia and explores types of and methods used in spousal killings. The research is based on court records in addition to laws, legal documents and other sources. Its preliminary conclusions include assumptions about scale of domestic violence, gender of perpetrators and victims, main trends in domestic homicide and their connections with available explanatory frameworks. The study reveals that Russian households were violent places accounting for different types of assaults and homicides, but in all these acts women died more frequently than men. Marital homicide occurred in all social groups in Russia. Motives and methods for marital homicides were consistent with gendered theories of power relations. Penal policies also reveal harsher treatment of women than men, pointing to the gendered definitions of power disciplining methods.     

Implementing the Foundations of Learning Project: Considerations for Preschool Intervention Research

While studies have documented the importance of strong implementation in intervention studies (e.g., see Durlak & Dupre, 2008 Durlak , J. A. , & Dupre , E. P. ( 2008 ). Implementation matters: A review of research on the influence of implementation on program outcomes and the factors affecting implementation . American Journal of Community Psychology , 41 , 327 – 350 .[Crossref], [PubMed], [Web of Science ®] , [Google Scholar]), more information is needed about how to ensure strong fidelity and quality of program implementation when delivering interventions under “real world conditions” and on a large scale. In this article, key lessons in implementing a demonstration and evaluation project known as the Foundations of Learning (FOL) demonstration are presented. Our discussion highlights several key components to the success of the project, including the building of effective coalitions before, and during, the delivery of the intervention to support the implementation process, as well as intensive, collaborative, and multilayered technical assistance provided as the intervention was delivered. Key lessons learned over the course of this project represent some that are highly consistent with prior research on this topic, as well as some that are unique, thus representing new areas for exploration in this burgeoning area of study.