Comparison of Different Analytical Methods for the Determination of Carbon Monoxide in Postmortem Blood

The determination of carbon monoxide (CO) and carboxyhemoglobin (COHb) is of utmost importance in forensic toxicology to determine the cause of death in cases of CO poisoning, fire, and explosions. To this end, reliable and updated analytical methods are required. In this paper, four different methods for the determination of carbon monoxide in postmortem blood samples were compared: (i) the spectrophotometric determination of COHb applying the method proposed by Rodkey and modified by Beutler–West, (ii) the spectrophotometric determination of CO using a micro-diffusion-based method, (iii) the determination of CO by gas chromatography coupled to a TCD detector, and (iv) the determination of COHb by blood gas analysis. Three postmortem blood samples were analyzed with all methods, and the results were comparable. The applied methodologies showed different features depending on the sensitivity, sample preparation, and volume. The HS-GC/TCD method in our hand was the most appropriate, on postmortem samples, and versatile to apply. Unfortunately, only a limited number of postmortem blood samples were available for this study due to the rarity of that kind of intoxication in our jurisdiction.     

俄罗斯经济恢复增长中军品、技术出口因素影响

俄罗斯经济经历了一段时期的衰退之后 ,从 1999年开始逐步回升。对于俄罗斯经济好转的原因 ,国内外学者做了很多分析。则从军品、技术出口成为俄罗斯经济恢复增长的影响因素之一角度 ,分析其经济增长的前景和影响。     

公安文书写作规律与技巧研究

公安机关奖惩性公文的写作规律和技巧。写作奖惩性公文,首先要选择文种;其次,嘉奖令、奖惩性决定和嘉奖批评性通报写作上有其共同的规律。法定公文与刑事法律文书写作特点有不同;此外,不管是向上级汇报工作、反映情况、答复上级的询问,还是向上级请求指示、批准事项,亦或是答复下级的请示事项,所使用的文种和行文要求都不同。最后,刑事法律文书写作所揭示的规律,完全可以用来指导治安法律文书的写作,当然,它们两者之间也有许多不同。     

影响警察学员中长跑技术运用因素的研究

本文采取文献资料法、调查法、访谈法等方法对辽宁警察学院、中国刑警学院的警察学员在学习中长跑技术时容易受到哪些因素的影响进行研究。结果表明,运动员在长时间跑步时的自身综合素质、主观心理、以及天气场地等都是影响其发挥技术的关键环节。     

交通管理专业开设《交通事故鉴定技术》可行性分析

交通事故鉴定技术是一门正在新兴的与广泛应用的科学,交通事故民警接受相关知识与技能教育将有助于提高事故现场调查取证与责任认定过程的客观性、科学性。在分析上述过程对鉴定技术需求必要性的基础上,指出在交通管理专业的教学体系上有必要设置该门课程,并对该门课程设置的时机与先行条件、教学内容与教学方法等进行了分析说明。     

Geomatic techniques in forensic science: A review

The purpose of this review paper is to highlight various geomatic techniques that crime scene reconstructionists or forensic practitioners can use to document different kinds of scenes, highlighting the advantages, disadvantages, and when best to use each technology. This paper explores geomatic techniques such as a total station, photogrammetry, laser scanners and structured light scanners and how they can be used to reconstruct crime scenes. The goal of this paper is not to discredit manual methods, as they are long standing and reliable, but instead to shed light on alternative methods that may produce equally or more accurate results with a more visually appealing final product. It is important for law enforcement and forensic professionals to understand the advantages and disadvantages of each technique, knowing when certain techniques should be used (and when they should not), and being able to revert to traditional methods if required.     

Significant association of TH01 allele 9.3 and SIDS

Sudden infant death syndrome (SIDS) constitutes a considerable percentage of infant death of unknown etiology. Individual catecholamine response variation is suspected to play a role in SIDS. TH01 is a tetrameric short tandem repeat marker in the tyrosine hydroxylase gene, which regulates gene expression and catecholamine production with allele 9.3 exerting a particularly strong effect on noradrenaline production. We investigated in an age-controlled study the TH01 allele frequencies in 127 cases of SIDS and 406 control cases to assess whether in SIDS cases a distinct TH01 allele distribution could be determined as has been reported by a previous study. We found that genotypes containing one or two 9.3 alleles were significantly more frequent in SIDS patients (58.2%) than in control subjects (48.4%, p=0.038), whereas all other alleles were more frequent in the control subjects. Our findings support the notion that there exists a significant association between TH01 gene configuration and SIDS.     

The effect of ionizing gamma radiation on natural and synthetic fibers and its implications for the forensic examination of fiber evidence

Circumstances of criminal activities involving radioactive materials may mean fiber evidence recovered from a crime scene could have been exposed to materials emitting ionizing radiation. The consequences of radiation exposed fibers on the result of the forensic analysis and interpretation is explored. The effect of exposure to 1-1000 kGy radiation doses in natural and synthetic fibers was noticeable using comparative forensic examination methods, such as optical microscopy, microspectrophotometry, and thin-layer chromatography. Fourier transform infrared spectroscopy analysis showed no signs of radiation-induced chemical changes in any of the fiber structures. The outcome of the comparative methods highlights the risk of "false negatives" associated in comparing colors of recovered fibers that may have been exposed to unknown radiation doses. Consideration of such results supports the requirement to know the context, including the environmental conditions, as much as possible before undertaking a forensic fiber examination.     

致病性大肠杆菌O_(157):H_7基因分型的研究进展

就目前的大肠杆菌O157:H7基因分型方法进行了综述,将其归纳为3类:基于DNA的限制性酶切技术、基于特异靶标的PCR扩增技术、基于基因组特定位点的DNA多态性分析技术,阐述和分析了这些技术所涉及的方法和技术特点。认为,大肠杆菌O157:H7具有重要的公共卫生学意义,开展流行病学调查和病原溯源追踪是防控大肠杆菌病的重要手段,而基因分型是大肠杆菌O157:H7鉴定和示踪的重要技术。     

荧光标记的ASP-PCR法对ABO基因分型的研究

目的建立一种方便准确的ABO基因分型检验方法。方法选取ABO基因外显子6和7上的3个位点nt261、nt297和nt803,分别对第6和7外显子进行扩增,并对扩增产物进行测序得出样本的基因型;然后用荧光标记的等位基因特异性引物对已知基因型的样本进行扩增,并用3130遗传分析仪进行电泳分析。结果该方法检出的基因型与测序得出的基因型完全一致。结论等位基因特异性引物法分型结果准确,特异性高,可用于法医学中对犯罪嫌疑人的筛查。