GFAP和VEGF在创伤性脑损伤中表达的研究进展

创伤性脑损伤是世界上导致死亡及残疾的主要原因之一。检测对中枢神经系统损伤有高度特异性和敏感性的生物标志物,可以在一定程度上反映损伤情况。胶质纤维酸性蛋白与血管内皮生长因子均可作为颅脑损伤的生物学标记物,其表达变化规律、峰值出现时间,与创伤性脑损伤的严重程度及损伤时间的推断密切相关,对于法医病理学具有重大意义。该文对胶质纤维酸性蛋白与血管内皮生长因子的分子生物学特性及其在创伤性脑损伤中的表达进行综述。     

大鼠脑损伤后EPO及其受体表达与损伤时间的关系

目的研究大鼠脑损伤后脑组织中促红细胞生成素(erythropoietin,EPO)及其受体(erythropoietin receptor,EPOR)的表达与损伤时间的关系。方法 72只SD大鼠随机分为对照组(36只)、脑损伤组(36只),在建模后1、2、4、8、12、24 h每组各取6只大鼠处死,取挫伤部位脑组织,应用实时荧光定量PCR和Western印迹法检测不同时间点EPO以及EPOR的mRNA表达和蛋白表达情况。结果伤后24h内,EPO及EPOR的表达随损伤时间的增加而呈上升趋势。EPO的mRNA及蛋白表达与损伤时间之间存在相关性,其相关系数分别为0.875、0.911(P0.05);EPOR的mRNA及蛋白表达与损伤时间之间存在相关性,其相关系数分别为0.936、0.905(P0.05)。结论大鼠颅脑损伤早期EPO及EPOR的表达呈逐步上升趋势,与损伤时间具有相关性,可以作为脑损伤时间推断的参考。     

大鼠急性肾缺血再灌注损伤早期的基因表达

目的研究急性肾缺血再灌注损伤早期的差异基因表达,探索其潜在的分子机制。方法通过单纯夹闭大鼠肾动脉制备肾缺血再灌注模型,进行基因表达芯片检测和生物信息学分析,筛选急性肾损伤早期的差异基因表达及其所涉及的细胞活动和信号通路。同时,通过构建差异表达基因的生物网络来寻找与急性肾损伤关系密切的分子,并进行荧光定量PCR验证。结果在检测出的151个差异表达基因中,132个基因显著上调,19个基因显著下调,GO与KEGG通路富集分析结果显示主要与细胞增殖、细胞因子介导的信号通路和免疫反应等有关。荧光定量PCR结果显示,与对照组相比,挑选出的3个与急性肾损伤高度相关的基因(ANXA1、PHLDA1、KLF6)在疾病早期具有显著的表达差异,上升倍数与芯片检测出的倍数基本一致。结论本研究揭示了急性肾损伤早期的差异基因表达特征以及所涉及的生物学过程和信号通路,其中ANXA1、PHLDA1、KLF6可能在急性肾损伤的发病机制中起到一定作用。     

论警察枪械致害之国家责任

《人民警察使用警械和武器条例》规定了警察违法或合法使用武器造成人员伤亡、财产损失时应该承担的法律责任。由于相关规范抽象模糊,以致在司法实践中滋生不少亟待解决的问题,有必要重新探讨警察用枪行为的性质,构建警察枪械致害赔偿案件统一的救济模式。同时,警察用枪合法与否的判断有赖于一套科学、公正的枪案调查制度。经客观、专业的枪案调查,如若警察用枪行为合法则,警察机关应承担国家补偿责任,相应的补偿渠道和补偿标准值得探讨。     

工伤赔偿与民事侵权赔偿的关系——从司法审判的角度审视

现代工业社会,工伤是经常发生的侵害劳动者权益的问题。工伤赔偿与民事赔偿请求权竞合时给司法审判带来了困难。理性处理二者的关系是和谐社会的必然要求。未来工伤保险立法必须对工伤保险赔偿与民事侵权赔偿之间的适用关系做出明确的界定,应根据工伤发生的原因而适用不同的处理模式。     

广泛软组织挫伤--挤压综合征

本文通过回顾国内外医学文献,对广泛性软组织挫伤--挤压综合征的研究现状和取得的成就进行了介绍,并对其今后的发展趋势进行了探讨.     

火器伤损伤程度的法医学鉴定(附63例报告)

目的　探讨火器损伤的致伤特点及活体损伤程度鉴定的有关问题。方法　 6 3例火器损伤主要按火器枪弹种类及数量分为四组 ,Ⅰ组 :霰弹损伤 ;Ⅱ组 :气枪损伤 ;Ⅲ组 :军用枪损伤 ;Ⅳ组 :射钉枪与爆炸损伤 ,对其进行前瞻与回顾性分析总结。结果　Ⅰ组损伤多见于斗殴 ,损伤部位以四肢部多见 ,异物存留、损伤程度明显高于其它各组 ;Ⅱ组损伤多见意外 ,不易造成异物存留 ;损伤程度轻于其它各组 ;Ⅲ组损伤多见抢劫与杀人 ,损伤部位多见头部 ;Ⅳ组损伤较少见仅占 7 92 %,不是本文讨论的重点。四组损伤合并脏器、神经血管损伤及骨折 33例 ,占 5 2 38%,构成重伤、轻伤 5 0例 ,占 79 37%。结论　火器损伤程度较重 ,致伤机理有其自身特点 ,现行损伤程度鉴定标准对其体现不够 ,值得法医同仁加以重视。     

原发性脑干损伤及其研究进展

本文综述了原发性脑干损伤的原因、临床表现、诊断与检测 ,重点介绍了其死亡原因和病理形态学 ,尤其是在细胞和分子水平上的改变 ,并对原发性脑干损伤的进一步研究方向进行了展望     

Spatial analysis of violent crimes in Metro Manila,Philippines

Spatial patterns of murder and physical injury in Metro Manila, Philippines were visualised through conditional choropleth maps. Relationship of both crime rates with some demographic variables were investigated while accounting for possible spatial autocorrelation using spatial lag models. Results show that both crime rates tend to cluster in the northern cities of Metro Manila. Furthermore, significant spatial lag coefficients were found only for physical injury rates, with values ranging from 0.49 to 0.62, signifying a positive city-level spatial dependence of physical injury rates in Metro Manila. Moreover, some demographic covariates, such as population density, percentage of young males, education, marriage, and immigration were found to be associated with both crime rates. These results could serve as useful indicators of crime incidence; thus it is recommended that crime monitoring systems include them to aid in resource allocation and program planning for better crime prevention and security management.     

Differences between Homicides Committed by Lone and Multiple Offenders in Korea

The aim of this study was to differentiate between homicides committed by multiple offenders and homicides committed by lone offenders. Using data on homicide incidents that occurred in South Korea between 1985 and 2008, we compared 134 homicides committed by multiple offenders, with 369 homicides committed by lone offenders. A greater proportion of homicides committed by multiple offenders involved injuries to the victim's head compared to homicides by lone offenders. Homicides committed by multiple offenders were more likely to involve blunt instruments and ligatures, whereas homicides by lone offenders were more likely to involve sharp instruments. In addition, a majority of the homicides committed by multiple offenders were planned. The results of this study have practical implications for homicide investigations, as well as theoretical implications for homicide research on the difference in offense behaviors based on the number of offenders.