用酶标抗人精液单克隆抗体A_(10)C_6建立ELISA斑点法快速鉴定人精(液)斑

作者用过氧化物酶标记自己研制的抗人精液单克隆抗体A10C6建立了简便快速鉴定人精斑的ELISA斑点法，直接通过酶标抗体上的酶催化底物显示颜色变化，来判断结果，阳性呈灰色斑点，阴性为无色。其结果只对人精斑浸出液显示阳性斑点，对人的其他组织器官及体液均呈阴性，对动物精斑也无交叉反应，精斑浸出液稀释到1：3200倍仍呈阳性结果。     

D6S2418在中国（汉族）、泰国和德国人群中的遗传多态性

目的获得D6S2418基因座的群体遗传学数据,分析其基因频率在不同群体间的分布情况是否存在差异,并分析其在法医学中的应用价值。方法采用PCR、非变性聚丙烯酰胺凝胶电泳(PAGE)及银染技术分析中国成都地区汉族、泰国曼谷地区泰国人群以及德国Maint地区德国人群中D6S2418基因座的遗传多态性,获得三个群体D6S2418基因座的群体遗传学数据。结果从300份分别采自成都地区汉族、泰国曼谷地区泰国人群以及德国Maint地区德国人群三个群体的无血缘关系个体的静脉血,共发现9个等位基因,观测到31种基因型。观测杂和度为64%～81%,个人识别机率为84.2%～93.5%,经统计学检验,基因型的频率分布符合Hardy-Weinberg平衡定律。等位基因频率的分布在三个群体间有显著差异。结论D6S2418基因座的个人识别能力高,在法医学个人识别和亲子鉴定应用中有较高价值。     

长白猪IL-6基因的克隆及其序列分析

用PHA和LPS诱导猪脾和淋巴结淋巴细胞,提取细胞总 RNA,利用 RT-PCR技术克隆猪IL-6的cDNA并进行序列测定与分析。测序结果及同源性分析表明,已扩增到 IL-6 基因,大小约为669 bp,含有1个639 bp的开放阅读框,编码212个氨基酸,其中前28个氨基酸为信号肽,且碱基序列在不同种属动物间差别较大。分组诱导表明,LPS效果更佳,PHA和 LPS联合诱导能使更多的IL-6转录表达。     

论十六大的主旨与宪政的发展

十六大所确立的主题和基本内容 ,为建设以民主政治为核心的宪政国家指引了方向。十六大报告的主旨将在六个方面对宪政的发展产生影响 :小康社会初步奠定宪政的社会基础 ;扩大基层民主是宪政建设的立足点 ;完善财产制是对宪政经济基础的巩固 ;对权力的制约和监督以及推进司法体制改革是宪政必备的两个要素 ;解决“三农”问题和深化分配制度改革是宪政实施的社会环境保障。     

大学英语评估系统的现状及改革建议

当前的大学英语教学评估系统包括三个子系统:基础阶段结束时的四、六级英语考试;各学期期末的学业考试;社会对大学生英语综合能力的需求和评价。但是,由于许多学校和用人单位都把四、六级考试作为考核的终极标准,导致现在的大学英语教学整个只是围绕四、六级考试进行,而四、六级考试本身又存在许多问题,造成了一种恶性循环;各学期期末的学业考试失去了其本身的意义,只是一种四、六级考试的模拟;学生也缺乏适当的途径去了解社会对英语运用能力的实际需求。这三个子系统对大学英语的教学产生了严重的负面反拨效应,要想对大学英语进行改革,首先就要从评估系统着手。     

Congressional Attention and Opportunity Structures: The Select Energy Independence and Global Warming Committee

This article examines the role that overlapping committee jurisdictions play in fostering or hindering attention to environmental and energy issues in the U.S. Congress. The Select Energy Independence and Global Warming Committee, created by House Democrats in 2007 , and its chairman Rep. Ed Markey took advantage of opportunities within the existing jurisdictional arrangement to increase attention to these issues despite lacking any legislative authority. Data from the 110th and 111th Congresses show that the Select Committee held more hearings on climate change and alternative energy, and on environmental and energy policy generally, than any other committee. The committee also increased the range of sources informing debate on these issues by calling different witnesses than other committees. These findings have important implications for the relationship between institutional structure and agendas and for Congress' policy‐making role on energy and the environment.     

Policy Adoption and Policy Intensity: Emergence of Climate Adaptation Planning in U.S. States

The United States is experiencing growing impacts of climate change but currently receives a limited policy response from its national leadership. Within this policy void, many state governments are stepping up and taking action on adaptation planning. Yet we know little about why some states adopt State Adaptation Plans (SAPs), while others do not. This article investigates factors that predict the emergence of SAPs, both in terms of policy adoption and policy intensity (goal ambitiousness). Applying the diffusion of innovation theory, I consider the relative influence of internal state characteristics, regional pressures, and test for conditional effects between government ideologies and severity of the problem. The results show interesting differences between predictors that influence policy adoption and ambitiousness. States are more motivated to adopt a policy when faced with greater climate vulnerability, have more liberal citizenry, and where governments have crossed policy hurdles by previously passing mitigation plans. The intensity of policies and goal setting, moreover, is more likely to be driven by interest group politics and diffuse through policy learning or sharing information among neighboring states in Environmental Protection Agency regions. These findings support an emerging scholarship that uses more complex dependent variables in policy analysis. These variables have the potential to differentiate symbolic from substantive policies and capture finer information about predictors of importance.     

Projecting the Arena of Inclusion: The Case of South Korea in Pursuing a Phased Inclusive Growth Process

South Korea created a mechanism that fuels inclusive growth, a process that coevolves economic growth and social well‐being. This study attempts to elaborate on the context and preconditions for development that would manifest salience policy implications for moving up the industrial value chain and attain inclusive growth. We contend against the view of simplified growth cum equity that is used to elucidate the success of Korea's socioeconomic development (economic miracles). Our findings informed us that there were intense measures taken throughout different phases of Korea's industrial development. In addition, we observed in many occasions intense negotiations between the state, firms, and civil society for social welfare and a better working environment. This led to a pursuit for inclusive growth in the post catching‐up phase that blended together many inclusive agendas, realizing growth that coevolved industrial upgrading and social welfare. This article seeks to explain how Korea populated its arena of inclusion in the process of pursuing rapid industrialization. The overview of different phases of development provides normative principles that are useful as a guide for other economies which aspire to attain similar development.     

Exploring the Effects of North–South and South–South Research Collaboration in Emerging Economies,the Colombian Case

International research collaboration (IRC) is associated with both positive and negative effects on the performance of research in emerging economies. While some authors claim that North–South collaborations improve scientific quality and visibility for Southern countries, others claim that it may entail the reorientation of research to comply with Northern agendas. South–South collaborations are thought to increase the focus on local affairs, therefore leading to a relatively small number of scientific international publications appearing in “high quality” journals. Research on the impact of IRC beyond publications in international journals has been neglected despite the importance of other products in knowledge creation. This research uses a broad range of scientific outputs to empirically assess such assumptions and explore the outcomes of IRC in Colombia. Results from multivariate regressions and nonparametric analyses show that, contrary to common assumptions, Colombian research teams collaborating with partners from the global South report higher scientific production, while those collaborating with Northern countries seem to contribute to local knowledge the most.