Reliable and Robust Molecular Sexing of the Hen Harrier (Circus cyaneus) Using PCR‐RFLP Analysis of the CHD 1 Gene

The hen harrier (Circus cyaneus) is a bird of prey that is persecuted in the United Kingdom, and there is a need for a DNA‐based individual identification and sexing system for the use in forensic investigations. This study reports a new set of PCR primers for the chromo‐helicase‐DNA‐binding protein 1 gene, which allows sexing using PCR‐RFLP. Instead of exonic primers that amplify across a large intron, this set consists of a primer within the intron, enabling reduction in amplicon sizes from 356 to 212 bp and 565 to 219 bp in W and Z chromosomes. DNA degradation and dilution experiments demonstrate that this set is significantly more robust than one that amplifies across the intron, and sequencing of the intronic primer‐binding region across several individuals shows that it is highly conserved. While our objective is to incorporate this primer set into an STR‐based individualization kit, it may in the meantime prove useful in forensic or conservation studies.     

Social Media and the 2014 Elections in Fiji

Abstract

After seven and half years Fiji returned to parliamentary democracy with elections on 17 September 2014. For the first time there was a ‘one’-day election, with the results confirmed a few days later. Reactions to the election results were swift, thanks to the media, particularly social media. While reactions to such events have often been sought from or dominated by political commentators and academics, a new trend emerged in post-election Fiji. Ordinary people through social media were actively participating in this process, extending conversations beyond their immediate family and community environments. Social media has been touted as a valuable tool for public participation. In Fiji the infancy of social media raises questions regarding whether it facilitates public participation and engagement, whether it has a place in Fiji’s new democracy and if it does, how it affects public discourse that, since December 2006, has been one-dimensional.