Genetic markers in human bone: I. Deoxyribonucleic acid (DNA) analysis.

Deoxyribonucleic acid (DNA) was isolated from a number of spongy and compact human bone tissue specimens, and the yield was estimated on a "per milligram of starting tissue" basis. DNA was, in addition, isolated from a number of corresponding blood and bone tissue specimens. Spectrophotofluorometry and ethidium bromide visualization on minigels were used to estimate the quantity and degree of degradation of DNA. The DNA from several blood-bone pairs is shown to give concordant restriction fragment length polymorphism (RFLP) typing results by two different typing protocols with five different single-locus probes. DNA from several additional blood-bone pairs is shown to give concordant results for human leucocyte antigen (HLA)-DQ alpha phenotypes following polymerase chain reaction (PCR) amplification and hybridization to specific allele-specific oligonucleotide (ASO) probes, and for the variable numbers of tandem repeats (VNTR) length polymorphisms 3' to the human apolipoprotein B (APOB) gene following PCR amplification with specific primers and analysis of the products by electrophoresis and ethidium bromide visualization.     

The only solution is revolution: the Spanish Confederación Nacional de Trabajo and the problem of unemployment in Republican Spain, 1931–1933

Although the Great Depression has been the subject of much research, focus normally centers on the impact instead of the tactics developed by working-class organizations to tackle the problems it caused, specifically unemployment. Recent research has sought to fill this gap, but numerous areas remain uncovered. This paper covers two of these: the situation in Spain and the reaction of anarcho-syndicalist union – the Confederación Nacional de Trabajo. Spain presents an anomalous case; a country that saw the replacement of a semi-fascist dictatorship by a democracy in the 1930s. Furthermore, the fact that the initial government of the Spanish Second Republic included the Socialists provides an opportunity of comparing and contrasting the positions and policies of reformist and revolutionary workers’ organizations. The study is based on predominantly on articles appearing in the CNT newspaper Solidaridad Obrera. The conclusion reached is that for the CNT rising unemployment was a symptom of an irreversible trend in a failing capitalist system, which could only be solved by the revolutionary overthrow of that system. Nonetheless, the union had to present plausible solutions to ameliorate the conditions of the workers to attract the unemployed and thus, create a force strong enough to lead that revolutionary change.     

Isolation of deoxyribonucleic acid (DNA) from saliva and forensic science samples containing saliva.

Saliva and saliva-stained materials were examined as potential sources of deoxyribonucleic acid (DNA) for DNA analysis and identity testing. In this paper, the authors demonstrate that DNA was isolated and DNA banding patterns suitable for DNA typing were obtained from fresh saliva and various saliva-stained materials, such as envelopes, buccal swabs, gags, and cigarettes. Furthermore, DNA and DNA banding patterns were obtained from actual forensic evidentiary samples containing mixed saliva/semen stains. The DNA banding patterns obtained from saliva or saliva-stained material were indistinguishable from the patterns obtained from blood or hair from the same individual. Intact DNA was readily isolated and DNA banding patterns were obtained from saliva stored at -20 degrees C and dried saliva stains stored under varying conditions. We conclude that saliva and saliva-stained material can be good sources of DNA for analysis and for DNA typing in certain forensic settings.     

Delay reduction in the federal courts: Rule 50(b) and the Federal Speedy Trial Act of 1974

During the 1970s, 94 federal district courts implemented two major policy initiatives, Rule 50(b) of theFederal Rules of Criminal Procedure and the Federal Speedy Trial Act, that were designed in Washington to combat delay in the processing of federal criminal cases. Both of these initiatives established a national priority of delay reduction in criminal cases, encouraged local district court planning for delay reduction; established reporting procedures for monitoring local compliance, and provided for the determination of quantitative goals for the time to disposition of criminal cases. Neither initiative mandated specific activities for delay reduction; this determination was left to the discretion of local federal district courts. This research examines the effectiveness of Rule 50(b) and the Speedy Trial Act by constructing a 150-month time series of three measures of case processing time. A multiple-intervention time-series model found that both of these initiatives contributed to the dramatic reduction in the time to disposition in federal criminal cases. These effects persisted after controls for changes in case characteristics and judicial resources were introduced.Points of view expressed in this research are those of the author and do not necessarily represent the official position of the U.S. Department of Justice.     

Sociodemographic variations in the linkage between emotion regulation and peer victimization

Sociodemographic variables may have important implications for understanding the role of global and discrete-level emotion regulation ability in the prediction of elementary school children’s peer victimization. We tested this hypothesis in a sample of 109 elementary school children from economically and racially diverse backgrounds. There was a positive relation between sadness regulation and overt victimization for children in early elementary grades, but not for children in later grades. In addition, there was a positive relation between sadness regulation and relational victimization for boys and a negative association between the two variables for girls. Girls were rated higher than boys on global emotion regulation ability. In addition, economically disadvantaged children reported more relational victimization than their more affluent peers. Affluent children also received higher ratings on global emotion regulation as well as sadness regulation. Anger regulation was also positively associated with overt victimization.