Analysis of ionic ratios in myocardial tissue and their relation to cardiac damage

The authors evaluated the usefulness of the postmortem biochemical analysis of ionic ratios in different parts of the heart and their relation to cardiac damage caused by chest trauma, as observed by anatomopathologic study. Fifty-nine 59 cases were studied, selected from routine necropsies, and samples were taken from different sites of cardiac tissue. The cause of death was trauma in 40 cases and nontraumatic causes in 19 cases. The object of this study was to analyze the levels of Na+, K+, Ca+2, Mg+2, and Zn+2 in different zones of the heart, and the relationship between intracellular and extracellular ion ratios and the different causes of death and any anatomopathologic alterations observed. The biochemical tests revealed a possible relation between the ionic values and cause of death. Alterations in cell membrane permeability and corresponding modification of the ionic ratios were produced earlier than histologic alterations, which need longer to establish themselves whether or not they follow a traumatic process.     

A Fatal Case of Anaphylactic Shock During Paragliding

Abstract: The growth in popularity of flying ultralight aircraft and paragliding has been associated with an increased involvement of Emergency Medical Services because of various types of trauma suffered from both inexperienced and skilled individuals. This case presentation reports on a paraglider pilot, who was seen spinning “unusually” rapidly toward the ground, without visible attempts to regain control of the aircraft. Besides the bilateral mydriasis and the absence of any ECG activity, there was a significant swelling of face, lips, neck, and tongue. Upon opening the mouth, a dead bee was found over the tongue, underneath the palate. A fatal anaphylactic shock was the likely cause of death of the pilot while still “in mid‐air.” This case is certainly different from the commonly reported accidents during paragliding. An updated review of the medical literature shows no reported cases of fatal anaphylactic shock during the practice of paragliding.     

Public-Sector Consolidated Financial Statements: An Analysis of the Comment Letters on IPSASB’s Exposure Draft No. 49

Public sector consolidated financial statements can be drafted according to different approaches, especially the “control approach” or “budgetary perspective.” Considering the growing importance of the International Public Sector Accounting Standards (IPSAS) in the international scenario, the aim of the article is to investigate these approaches through an analysis of the comment letters submitted to the Exposure Draft no. 49, which will replace the current IPSAS 6. The main finding concerns the acceptance of the key role played by the control approach, even though it needs to be better specified, in order to take into account the public sector characteristics.     

Pulmonary Cement Embolism After Kyphoplasty

Pulmonary polymethylmethacrylate (PMMA) cement embolism after kyphoplasty (KP) surgery is a quite frequent event as well as the pulmonary embolization of central venous catheter fragment. This report shows the case of a subject who, after KP, developed pulmonary embolism and who underwent thoracic surgery. After hospital discharge, the subject advanced a claim for damages toward the hospital where he was operated, complaining sensation of tenderness at the chest surgical scar and esthetic damage. To understand the nature of the embolism (either central venous catheter fragment or cement), chemical investigations were then ordered. Spectrometry identified the PMMA cement used for KP. When doubts rise about the origin of the embolized material, chemical investigations may reveal important data not only for clinical but also for forensic purposes.     

A standard procedure for accommodating forensic anthropological and genetic analysis of decomposing human remains from tropical climates

At the Medical Legal Center in Ribeirão Preto, Brazil (CEMEL/FMRP-USP), unidentified decomposing bodies routinely undergo soft tissue removal (by immersion in water at 80–90 °C for 24 h) prior to an anthropological analysis intended to yield a biological profile of age, sex, ancestry, height, pathology and so on. In the event that this analysis is unsuccessful, samples may be submitted for DNA profiling. The tropical climate and the defleshing process may confound preservation, recovery and analysis of DNA, however. In order to establish an optimal standardized protocol for identification of decomposing human remains from a tropical climatic region, the outcome of anthropological and genetic analyses was compared, along with the utility of bone (mainly femur and sternum) and teeth (mainly molar) specimens for DNA analysis. In a sample (n = 39) of partially skeletonized remains, anthropological analysis was sufficient for identification in eight cases. In further six cases, DNA profiling was successfully attempted. As a consequence of our study, we recommend collection of 1–2 well preserved teeth prior to defleshing and anthropological analysis in these circumstances.     

Developmental Pathways in Youth Sexual Aggression and Delinquency: Risk Factors and Mediators

Childhood exposure to violence against females and male-modeled antisocial behavior were examined as risk factors for sexual aggression, and nonsexual aggression and delinquency, in a sample of 182 adolescent male sex offenders using structural equation modeling. Both risk factors produced direct and indirect effects on nonsexual aggression and delinquency with Psychosocial Deficits and Egotistical–Antagonistic Masculinity playing important mediating roles. Exposure to violence against females helped explain sexual aggression through the mediating role of Psychosocial Deficits. As hypothesized, youth who sexually offended against prepubescent children manifested greater deficits in psychosocial functioning, committed fewer offenses against strangers, and demonstrated less violence in their sexual offending than offenders against pubescent females. Findings are discussed within the context of two major evolutionary psychological concepts for explaining human sexual behavior: intrasexual selection and intersexual selection.     

Nuclear DNA typing from ancient teeth

Because of the adverse effects that diagenesis exert on ancient skeletal remains, DNA from these samples is often compromised to the point where genetic typing can be challenging. Nevertheless, robust and reliable methods are currently available to allow successful genotyping of ancient specimens. Here we report nuclear DNA-based methods and typing strategies used to analyze 2 human skeletons from a medieval burial. Reliable DNA nuclear profiles were obtained from teeth, whereas mitochondrial DNA analyses in bones were inconclusive. A complete nuclear mini short tandem repeat profile was obtained from a well-preserved premolar, but only a partial one from the femur. Increasing the sensitivity of the polymerase chain reaction system allowed a full profile from the latter, but the presence of artifacts reinforced the idea that the interpretation of this kind of analysis must be performed with caution. The results presented here also indicate that DNA from dental pieces can be better preserved than from bones, even in the case of well-preserved long bones with thick cortical tissue such as the femurs, and have a better chance of successful genetic typing, probably because of the high degree of protection conferred to the DNA by the enamel.     

Non-Sexual Delinquency in Juvenile Sexual Offenders: The Mediating and Moderating Influences of Emotional Empathy

The theoretical linkage of empathy to sexually aggressive and antisocial behavior is reviewed, and assessment conducted on the role of emotional empathy in the non-sexual delinquent behavior of juvenile sexual offenders. In examination of developmental antecedents of empathy, self-reported parental attachment and positive fathering experiences were found to be positively associated with emotional empathy, while reported exposure to violence against females was inversely related. As hypothesized, emotional empathy was found to have both mediating and moderating influences on risk of engagement in non-sexual delinquency. Emotional empathy was found to be negatively associated with non-sexual delinquency and to partially mediate the positive influences of exposure to violence against females and hostile masculinity. Emotional empathy was also found to function as a moderator of hostile masculinity, with high empathy levels associated with an attenuated positive effect of hostile masculinity on non-sexual delinquency, and low levels with an accentuated effect. Possible mechanisms for this moderating influence are discussed, along with clinical implications of the findings and directions for future research.     

Advantage of affinity histochemistry combined with histology to investigate death causes: indications from sample cases

Mast cell histochemistry has been proposed in addition to classic histological methods to estimate the course of traumatic events before and after death. We have addressed the utility of this approach on nine victims of different types of trauma. Sections of wounded skin were stained with hematoxylin and eosin and with fluorescent avidin to tag mast cells. Mast cell numbers were evaluated by both direct and digitalized counts. Intact skin was used as control. The results on mast cells implemented the findings upon hematoxylin and eosin stain and helped to put the wounds and death in chronological sequence. Digitalized morphometry allowed to reduce intra- and inter-observer variation. We conclude that combined histological and histochemical analyses can be of practical use in forensic pathology, that a preliminary setting of the reference values is needed for each laboratory, and that image analysis can be of help for the quantification of the results.