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Silvia Turroni Beatrice Vitali Marco Candela Paolo Gionchetti Fernando Rizzello Massimo Campieri Patrizia Brigidi 《党史博采》2010,(1)
AIM:To profile protein expression in mucosal biopsies from patients with chronic refractory pouchitis following antibiotic or probiotic treatment,using a comparative proteomic approach. METHODS:Two-dimensional polyacrylamide gel electrophoresis and matrix-assisted laser desorption/ ionization-time of flight mass spectrometry were used to characterize the changes related to antibiotic therapy in the protein expression profiles of biopsy samples from patients with chronic refractory pouchitis.The same proteom... 相似文献
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Vitali Silitski 《Communist and Post》2010,43(4):339-350
This article examines the internal and external reactions by post-Soviet autocrats to the colored revolutions. First, the colored revolutions provoked incumbents in Azerbaijan, Belarus, Kazakhstan, Russia, and other post-Soviet countries to strengthen domestic efforts to preempt opposition challenges. Preemptive measures included restrictions on independent civil society, disruptions of independent electoral monitoring, promotion of pro-government civil society groups, and assaults on opposition and democracy assistance. Such actions, together with already existing endowments, allowed these regimes to survive. Next, the colored revolutions stimulated increased coordination among non-democratic states to squash opposition. Measures included counter-monitoring of elections to offset Western claims of fraud, and increased efforts at military and economic cooperation such as the Shanghai Cooperation Organization. Simultaneously, Russia sought to destabilize Georgia and Ukraine through economic and, in Georgia, military pressure. This authoritarian backlash following the colored revolutions buttressed the surviving autocracies, which prospects for democratization have become even more bleak and distant. 相似文献
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Conventional confirmatory biochemical tests used in the forensic analysis of body fluid traces found at a crime scene are destructive and not universal. Recently, we reported on the application of near-infrared (NIR) Raman microspectroscopy for non-destructive confirmatory identification of pure blood, saliva, semen, vaginal fluid and sweat. Here we expand the method to include dry mixtures of semen and blood. A classification algorithm was developed for differentiating pure body fluids and their mixtures. The classification methodology is based on an effective combination of Support Vector Machine (SVM) regression (data selection) and SVM Discriminant Analysis of preprocessed experimental Raman spectra collected using an automatic mapping of the sample. This extensive cross-validation of the obtained results demonstrated that the detection limit of the minor contributor is as low as a few percent. The developed methodology can be further expanded to any binary mixture of complex solutions, including but not limited to mixtures of other body fluids. 相似文献
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Lukka M Tasa G Ellonen P Moilanen K Vassiljev V Ulmanen I 《Forensic science international》2006,164(1):3-9
We report triallelic patterns in several short tandem repeat (STR) loci revealed by routine paternity testing using the commercial AMPFlSTR Profiler and AMPFlSTR SGMplus kits. One case where the TPOX-locus (2p25.3) produced three peaks from the blood sample of a child was analysed further. Quantitative polymerase chain reaction (QPCR) and STR typing of the DNAs of the family trio revealed a large (>1.59 Mb) duplication flanking the TPOX-locus in chromosome 2 in both the mother and child. The implications of such genetic anomalies for paternity testing are discussed. 相似文献
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Aliaksandra Sikirzhytskaya M.Sc. Vitali Sikirzhytski Ph.D. Gregory McLaughlin M.Sc. Igor K. Lednev Ph.D. 《Journal of forensic sciences》2013,58(5):1141-1148
Body fluid traces recovered at crime scenes are among the most common and important types of forensic evidence. However, the ability to characterize a biological stain at a crime scene nondestructively has not yet been demonstrated. Here, we expand the Raman spectroscopic approach for the identification of dry traces of pure body fluids to address the problem of heterogeneous contamination, which can impair the performance of conventional methods. The concept of multidimensional Raman signatures was utilized for the identification of blood in dry traces contaminated with sand, dust, and soil. Multiple Raman spectra were acquired from the samples via automatic scanning, and the contribution of blood was evaluated through the fitting quality using spectroscopic signature components. The spatial mapping technique allowed for detection of “hot spots” dominated by blood contribution. The proposed method has great potential for blood identification in highly contaminated samples. 相似文献
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