A novel multiplex PCR system consisting of Y-STRs DYS441, DYS442, DYS443, DYS444, and DYS445

We have developed a new sensitive multiplex PCR system consisting of five male-specific and polymorphic tetranucleotide STRs--DYS441 (GDB: 10013873), DYS442 (GDB: 10030304), DYS443 (GDB: 10807127), DYS444 (GDB: 10807128), and DYS445 (GDB: 10807129) on the Y chromosome. Fifty pg DNA per 10 microL reaction volume was required for the correct typing of five STRs. Using this system, the five Y-STRs were correctly typed from blood and semen stains that had been stored for several years at room temperature.     

Sensitive fluorometric detection of heat-stable alkaline phosphatase from bloodstains for the forensic diagnosis of pregnancy

Sensitive and simple procedures are established for the forensic pregnancy test from bloodstains. This paper describes new techniques based on heat stability of placental alkaline phosphatase. In this study the total and heat-stable alkaline phosphatase activities were determined using 4-methylumbelliferyl-phosphate as substrate. The ratio of heatstable alkaline phosphatase to total alkaline phosphatase was calculated. A more simple screening test which was visible by exposure to UV light is described. These may be helpful in the diagnosis of pregnancy from only a slight bloodstain.     

Ideology and Voter Preferences as Determinants of Financial Globalization

We propose that the global spread of ideas affects international economic openness policies, and that to omit ideology as an explanatory variable for economic globalization is to risk omitted variable bias. Using voting data, we create measures of global ideology regarding economic openness and propose that changes in both global and domestic ideology influence how open or closed to international finance an economy is. We also test other influences on liberalization, including proposed state-centered diffusion mechanisms. Using PCSTS and system-GMM models, we estimate the determinants of change in international capital account regulation for 82 countries, 1955 to 1999. We thereby examine diffusion of both liberalizations (1950s and 1990s) and closures (1960s and 1970s). Changes in both global and domestic ideology robustly influence liberalization and closure. The capital account policies of neighboring countries (positively) and of the leading economies (negatively) also influenced a country's capital account liberalization.     

Resistance and reform: discourses on marital law in Japan*

This article examines opinions concerning fūfubessei (a married couple retaining their birth surnames) posted on an online forum. Recently, the topic of fūfubessei has once again come under a spotlight, since the Japanese Supreme Court upheld the constitutionality of the Civil Code Article 750, which stipulates that a married couple must choose either the husband's or wife's surname upon marriage registration. Owing to the large number of women forfeiting their surnames, the fūfubessei issue has often been researched from feminist perspectives, which may have hindered the voices of others. This article analyzes, using text mining (quantitative analysis) and discourse analysis (qualitative analysis), a large number of opinions from people of various cultural backgrounds. The results of this study reveal a diversity of beliefs and attitudes towards Article 750, reflecting the complexity of the issue. In general, proponents of reform claim the law violates equality, rights and liberty. From opponents there was a strong push to conform rather than to recognize a need for individual choice. Moreover, the many personal narratives provided insight into issues on the ground and revealed how many in Japanese society have dealt with the law, social pressure and social expectations and why they have followed the path they have chosen.     

The structure of medical malpractice decision-making in Japan

The article summarises the problems in the medical malpractice litigation systems in the United Kingdom and Japan, demonstrating the similarities and identifying the length of time between initiating an action and its decision and other factors responsible for lengthy litigation. Based on analysis of decisions of medical malpractice cases between 1986 and 1998 in Japan, the functioning of the Japanese medical malpractice litigation system is discussed. Lengthy litigation is shown to be correlated with outcome and implies that the Japanese medical dispute resolution mechanism favours those who can endure lengthy litigation, namely the defendants, who are physicians or hospitals. In view of the similarities between the two systems, it is likely that the same bias--that the wealthier party in the litigation is more likely to win the case--also occurs in medical malpractice litigation in the United Kingdom and Australia.