Isolation of spermatozoa in vaginal contents by centrifugation in a colloidal silica gradient

Human spermatozoa were separated from vaginal contents with epithelial cells by density gradient centrifugation in colloidal silica coated with polyvinylpyrrolidone. In this procedure spermatozoa were collected at a density range of 1.130 - 1.142 g/ml by centrifugation at 35 000 g for 1 h.     

Identification of impurities and statistical classification of methamphetamine tablets (Ya-Ba) seized in Thailand

Impurity profiles of methamphetamine tablets seized in Thailand have been investigated. The samples are extracted with small amounts of ethyl acetate under alkaline condition and the extracts are analyzed by capillary gas chromatography. Nine compounds (1,2-dimethyl-3-phenylaziridine, ephedrine, methylephedrine, N-formylmethamphetamine, N-acetylmethamphetamine, N-formylephedrine, N-acetylephedrine, N,O-diacetylephedrie, methamphetamine dimer) are identified as impurities in methamphetamine tablet. Caffeine and ethyl vanillin are also detected as diluents and/or adulterants, and acetylcodeine monoacetylmorphine and diacetylmorphine are contained in many samples. In addition, trans-3,4-dimethyl-5-phenyl-2-oxazolidone is newly found as an impurity. For characterization and comparison of methamphetamine tablet exhibits, intensely and commonly detectable nine peaks are selected as the factor for multivariate analysis. The procedures reported here permit classification of 250 analyzed exhibits into five groups and characterization of classified groups.     

Analysis of AmpFlSTR MiniFiler™ loci and its forensic application

The allele frequencies of eight MiniFiler™ loci have been analyzed in 101 Japanese individuals living in Kanagawa with informed consent by means of ABI 310 Genetic Analyzer. A total of 7 alleles for D13S317, 8 alleles for D7S820, 11 alleles for D2S1338, 11 alleles for D21S11, 5 alleles for D16S539, 14 alleles for D18S51, 8 alleles for CSF1PO, and 13 alleles for FGA were observed. The polymorphic profiles of these MiniFiler™ loci in the present study were essentially the same as those obtained by using the AmpFlSTR^® Identifiler^® PCR Amplification kit. The combined matching probability of eight MiniFiler™ loci and cumulative probability of paternity exclusion were estimated as 1.97 × 10⁻¹⁰ and 0.9996, respectively. The MiniFiler™ kit was useful for individual identification in forensic analysis.     

Case report: Etizolam and its major metabolites in two unnatural death cases

A simultaneous analytical method for etizolam and its main metabolites (alpha-hydroxyetizolam and 8-hydroxyetizolam) in whole blood was developed using solid-phase extraction, TMS derivatization and ion trap gas chromatography tandem mass spectrometry (GC-MS/MS). Separation of etizolam, TMS derivatives of alpha-hydroxyetizolam and 8-hydroxyetizolam and fludiazepam as internal standard was performed within about 17 min. The inter-day precision evaluated at the concentration of 50 ng/mL etizolam, alpha-hydroxyetizolam and 8-hydroxyetizolam was evaluated 8.6, 6.4 and 8.0% respectively. Linearity occurred over the range in 5-50 ng/mL. This method is satisfactory for clinical and forensic purposes. This method was applied to two unnatural death cases suspected to involve etizolam. Etizolam and its two metabolites were detected in these cases.     

Assessment of non-timber forest products in Phnom Kok community forest, Cambodia

Our study focused on the potential revenues of non-timber forest products (NTFPs) in the Phnom Kok forest community in Cambodia. Questionnaires were administered at the beginning of 2007, and results showed that people collected plant- and animal-based NTFPs seasonally. Plant-based NTFPs collected included fodder, food, medicines, resin, construction materials, and ingredients for cosmetics. Animal-based NTFPs collected included honey and beeswax, live animals, bushmeat, medicines, and hides (skins). NTFPs are important economic and natural resources in community forests, and they are used for both family consumption and commercial trade. Resin products represented the largest source of income, followed by honey and beeswax and live animals. Overexploitation of NTFPs, forest degradation, and anarchic forest clearing through fire pose great threats to the continued production of NTFPs. Therefore, sustainable management techniques must be implemented and harvesting should be regulated. Local residents and leaders need to be educated about the long-term benefits of the forest and NTFPs in preserving the environment.     

Effective injection in pulsed splitless mode for impurity profiling of methamphetamine crystal by GC or GC/MS

Gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS) are commonly used for the impurity profiling of illegal drugs. For the impurity profiling of methamphetamine, it is very important to obtain information about impurities related to the manufacturing route and the precursor chemicals [B. Remberg, A.H. Stead, Drug characterization/impurity profiling, with special focus on methamphetamine: recent work of the United Nations International Drug Control Programme, Bull. Narcotics LI (1999) 97-117 ]. There are many artifact impurities arising from the preparation of samples and conditions of GC. Moreover, some impurities pose a barrier to the statistical processing of methamphetamine profiling. We investigated capillary GC analysis using pulsed splitless (PS/L) injection to minimize the thermal decomposition of impurities at the injection port and improve the transfer of samples into the column. We confirmed that the optimal conditions of PS/L-mode are 230 degrees C (injection temperature), 50 psi (pulsed pressure) and 1.1 min (pulsed time) for the methamphetamine profiling. Based on the impurity profiles of 48 methamphetamine crystals in PS/L-mode, we can achieve very easy handling and obtained, good results.     

Disquieting growth: urban space in Kawashima Yūzō's film Suzaki Paradise Red Light

This article examines how Japanese postwar cinematic texts manifest and comment upon contemporary political and economic events, and considers the usefulness of cinema for a more complete historical understanding of the period. In particular, it argues for the significance of fūzoku eiga, or ‘films of customs and manners’ by analyzing a representative text of that genre, Kawashima Yūzō's 1956 film Suzaki Paradise Red Light. Although Kawashima's film has been treated as an apolitical melodrama, a close textual analysis reveals it to be a counter-narrative to the success story of postwar economic recovery and growth that the Japanese state sought to promote. Key to this analysis is an examination of the urban space of the Suzaki neighborhood in Tokyo, as depicted in the film. Kawashima's tour of Suzaki addresses the issues of the economic stagnation within the metropolis, uneven development, and the liminal space of muen, or ‘no ties,’ which offers a brief refuge from an increasingly disciplined everyday life.     

Three cases of sudden death due to butane or propane gas inhalation: analysis of tissues for gas components

We report three cases of sudden death due to inhalation of portable cooking stove fuel (case 1), cigarette lighter fuel (case 2), and liquefied petroleum gas (LPG) (case 3). Specimens of blood, urine, stomach contents, brain, heart, lung, liver, kidney, and fat were collected and analyzed for propylene, propane, isobutane, and n-butane by headspace gas chromatography. n-Butane was the major substance among the volatiles found in the tissues of cases 1 and 2, and propane was the major substance in case 3. A combination of the autopsy findings and the gas analysis results revealed that the cause of death was ventricular fibrillation induced by hard muscle exercise after gas inhalation in cases 1 and 2, and that the cause of death in case 3 might be hypoxia. It is possible that the victim in case 3 was under anesthetic toxicity of accumulated isobutane which is a minor component of liquefied petroleum gas.     

The estimation of the time of death by non-protein nitrogen (NPN) in cadaveric materials. Report 3: Multiple regression analysis of NPN values in human cadaveric materials

The non-protein nitrogen (NPN) values in brain, lung, liver, and kidney in 79 autopsy cases were determined according to the Micro-Kjeldahl Nessler method. Multiple regression analysis of the data was performed with every possible combination of the time of death and the NPN values in the tissues.The brain NPN showed the best correlation with the postmortem time (r = 0.673), whereas the other correlations were less satisfactory (lung r = 0.422, liver r = 0.397, and kidney r = 0.379, respectively). However, multiple combinations of each tissue NPN value proved to give better correlation coefficients and smaller errors of the estimated time of death.The practical significance of the tissue NPN as a postmortem biochemical indicator of the time of death and the multiple regression analysis of such indicators were extensively discussed in this report.     

日本社会的结构变化与高等教育的课题

20世纪 90年代 ,日本面临着全球化背景下知识社会的新挑战。因此 ,日本的高等教育不但一方面要把注意力集中在其中的一些结构性问题上 ,同时要满足来自于经济和社会变革的需求。日本政府为此提出了一系列高等教育改革措施。东京大学正在落实的改革有三个方面。第一是管理 ,包括学校管理和财政。目前 ,政府倡导要求国立大学具有更大的自主性 ,给校长更大的权力。虽然这似乎是一种普遍的方向 ,但是 ,政府详细的改革计划也带来了各种各样的问题。第二个改革领域是研究。大学采取措施加强与工业界的关系 ,加强国际关系网络。第三 ,教育 ,特别是本科生层次的教育是大学非常重要的任务之一。然而 ,在一个以研究为中心的大学 ,提高教育质量不是一件容易的事。东京大学已经着手采取措施改进本科生教育