Hair analysis for drug abuse: I. Determination of methamphetamine and amphetamine in hair by stable isotope dilution gas chromatography/mass spectrometry method

Determination of methamphetamine and amphetamine in hair was performed by gas chromatography/mass spectrometry using stable isotope-labeled internal standards, 2-methylamino-1-phenylpropane-2,3,3,3-d4 and 2-amino-1-phenylpropane-2,3,3,3-d4. Extraction of hair with methanol/5M hydrochloric acid (20:1) using ultrasonication was chosen as the standard method. The calibration curves for amphetamines in the hair were linear from 1 to 100 ng/mg (r greater than 0.99). The detection limit was 0.5 ng/mg at the 95% confidence level. The coefficients of variation (CV) (n = 8) of analysis using the spiked hair with methamphetamine were from 0.7 to 6%. The CV (n = 8) of analysis of the methamphetamine abuser's hair was 17.5%. Sectional analysis of monkey and human hair after methamphetamine ingestion suggested a good correlation between the duration of drug use and drug distribution in the hair.     

Sex determination from buccal mucosa and hair root by the combined treatment of quinacrine staining and the fluorescent Feulgen reaction using a single specimen

In order to determine sex using a single specimen, buccal mucosa and hair roots obtained from male and female individuals were used. The specimens were first stained with quinacrine for the detection of the Y-chromatin, and subsequently were stained by the fluorescent Feulgen reaction using acriflavine for the detection of the X-chromatin. In the male specimens, the frequency of fluorescent spots of quinacrine-positive bodies was high, whereas that of acriflavine-positive spots was low. On the other hand, in the female specimens, the frequency of quinacrine-positive spots was very low, while that of the acriflavine-positive spots was high. These specimens were air-dried and were allowed to stand at room temperature for periodical observations. The result was that sex difference was distinguishable for 4 months by the combined treatment method.     

Suicide bombers in Western literature: demythologizing a mythic discourse

In facing the challenge of suicide terrorism today on a global scale, it is tempting to attribute the phenomenon to cultural or religious behavior patterns observed solely in non‐Western countries. Yet recent scholarly research on its possible cultural or religious origins has led less to convincing and satisfactory results than to an unresolved aporia between arbitrariness and contingency. By the same token, little notice is taken of the fact that, in Western history and literature, actions and figures can be found whose development and strategies conspicuously resemble those of today’s suicide bombers. A series of such analogous examples in Western literature from antiquity to the present is explored in order to provide deeper insights into situational and systemic factors at work beyond cultural and religious determinants. To more effectively counter suicide terrorism, alternative policies are proposed with the vision of a peaceful coexistence of diverse cultures and religions.     

Putin's foreign policy toward North Korea

Since coming to office in 2000, President of the Russian Federation,Vladimir Putin, has taken a pragmatic and calculating approachto the conduct of foreign policy in order to promote Russia'sgeopolitical and economic interests. For him, a higher profileon Korean issues has been an opportunity to show that Moscowcan influence Pyongyang and play a meaningful role in greatpower diplomacy on the Korean peninsula. As a result of activediplomacy toward North Korea, the two former communist alliesput a period to the deteriorating relations in the 1990s; afterthe September 11 terrorists attack and the launch of the waron terrorism Moscow's influence over Pyongyang reached its peak.The beginning of the North Korean nuclear crisis in October2002, however, relegated Moscow to a marginalized position inthe Korean issue. This paper examines how Putin's pragmaticdiplomacy was put into effect in Russia's foreign policy towardNorth Korea and how circumstances on the Korean peninsula haveaffected Russia–DPRK bilateral relations. An analysisof these factors shows why Russia's role in the ongoing Six-Partyprocess has become insignificant. At the present time, it iscertainly plausible that Russia will not activate diplomaticefforts to solve the North Korean nuclear crisis and will besatisfied with merely securing a seat at the multilateral talksin Northeast Asia.     

Confrontation or coexistence: The national reconciliation process in Cambodia

On October 23, 1991, the four warring factions in Cambodia signed a peace accord, agreeing to disarm and allow UN‐sponsored elections. Through the UN Transitional Authority in Cambodia (UNTAC), elections were held in May 1993, despite disruptive efforts by some factions. Yet conflict continues. Professor Yasuhiro Takeda of Japan's Defense University examines current possibilities for reconciliation between the Khmer Rouge and the new government in Cambodia.     

The Political Economy of Familial Relations: The Japanese State and Families in a Changing Political Economy

This article explores the ongoing negotiation over the boundaries between the world of political economy and families by analysing the reactions and responses of the Japanese government and feminists to changes in the political economy as well as popular patterns of family forming since the 1990s. This negotiation has occurred through the transition in the political economy brought about by globalisation and neo-liberal political reforms, and the re-calibration of family and gender roles has emerged as its primary ground. As a result, Japanese women are being required to make a hasty leap from the Fordist model of family life to a more self-steering idea of the individual. This ineluctably re-calibrates the bio-political arrangement into a more advanced mechanism, while discussion of the ‘ethics of care’ has been left relatively absent. Taking up these issues, this article discusses the implications of a changing political economy on Japanese families.     

Hair analysis for drug abuse, Part II. Hair analysis for monitoring of methamphetamine abuse by isotope dilution gas chromatography/mass spectrometry

Sectional analysis of methamphetamine abuser's hair was performed by using stable-isotope dilution GC/MS method. Drug concentrations of hair shaft cut into 2-cm sections from the root side were compared with the self-reported drug histories of 11 cases and the results of experiments on monkeys. It was found that in nine of the 11 cases, the relationship between the results of sectional analysis and drug histories coincided, but the sectional analyses of two cases were not consistent with self-reported drug history. The difference in drug concentrations between the regions of scalp hair was also investigated. Our study suggests that hair analysis, especially sectional analysis, may be useful in determining past drug history even though it is not exact.     

Sex determination from plucked human hairs without epithelial root sheath. II. Depigmentation of melanin in the hair cortex before Feulgen reaction

In hair roots devoid of the epithelial root sheath, an attempt was made to decolorize the melanin granules without affecting the Feulgen reaction for the sex chromatin. The hair samples were treated with 0.25% potassium permanganate for 1 hour, 0.3% hydrogen peroxide for 1 minute and 5% oxalic acid for 5 minutes, and then stained with Feulgen. The frequency of sex chromatins ranged from 22% to 47% (average 32%) in female samples and from 0% to 8% (average 5%) in male samples. Thus, the frequency distributions of the male and female samples were completely independent of each other. The sex chromatins in dried female hairs were detectable at a frequency of 16 – 26% several weeks after plucking. The depigmentation procedure almost completely bleached the melanin granules in the hair cortex, and produced no harmful effect on the Feulgen reaction that followed.