A new 39-plex analysis method for SNPs including 15 blood group loci

A novel 39-plex typing system for single nucleotide polymorphisms (SNPs) has been developed. This multiplex approach has the advantage of being able to type 38 autosomal SNPs and one sex-discriminating base exchange site on the X and Y chromosomes rapidly and simultaneously. The SNP loci on the autosomes, which we examined, contain 15 loci distributed on blood type genes: three on RhCE, two each on Km and Gc, and one each on Duffy, AcP1, Tf, MN, GPT, EsD, PI, and Kidd genes. Thirty-seven genomic DNA fragments containing a total of 38 SNPs and one sex-discriminating site were amplified in one multiplex PCR reaction. Following the reaction, single nucleotide primer extension reaction was performed by dividing these SNP loci into five groups. The SNP type of each of the 39 loci was determined at one time by capillary electrophoresis using the newly designed multi-injection method. The combined PD (power of discrimination) of this typing system was (1-1.1) x 10(-14), and the MEC (mean exclusion chance) was 0.9990. We applied this system to forensic cases, including 16 paternity testing cases (13 non-exclusion and three exclusion cases) and one personal identification case. For the paternity testing cases, the highest Essen-M?ller's W-value was 0.9999995. The pM (matching probability) of the personal identification case was 2.22 x 10(-17). These data showed that this system was an excellent tool for use in forensic cases of paternity testing and personal identification.     

Japanese Suspect Interviews,Confessions, and Related Factors

Two hundred and ninety-one new adult male inmates convicted of murder, robbery, arson, rape, forcible indecency, and kidnapping completed questionnaires about their pretrial interviews. Logistic regression analyses revealed that marital status, feelings of guilt, and perceptions of the strength of the evidence significantly affected the likelihood of confession by those who had already decided to confess prior to interview. By contrast, prior arrest history and interview style affected the likelihood of confession by those who had not previously decided to confess. Suspects who had no previous arrests and had undergone either Undifferentiated-high or Relationship-focused interviews were more likely to make full confessions. In Undifferentiated-high interviews, police officers employed all of the interviewing techniques examined: they listened closely to the suspects’ accounts, attempted to build good relationships, and discussed the crimes, while also presenting evidence and confronting the suspects. In Relationship-focused interviews, police officers listened attentively to the offenders’ accounts, tried to build good relationships, and discussed the crimes directly. Prisoners who experienced Relationship-focused interviews felt satisfied with their confessions and admitted guilt at trial.     

Police interviewing styles and confessions in Japan

Psychological studies of suspects' confessions have been conducted mostly in English-speaking and European countries, and the results may not generalise to countries whose cultures and policing practices differ. In particular, the difference between Japanese and Western laws may affect the roles that police interviewers play in suspects' confessions. This study examined the interviewing techniques used by Japanese police officers and associated features of the suspects' confessions. An extensive questionnaire was completed by 276 police officers across Japan. Detailed ratings of their interview techniques were factor analysed, yielding five factors: Presentation of Evidence, Confrontation, Rapport Building, Active Listening, and Discussion of the Crime. Based on these five factors, we identified four interviewing styles: Evidence-focused, Confrontational, Relationship-focused, and Undifferentiated. When interrogators employed the Relationship-focused interviewing style, suspects were more likely to make full confessions and to provide new information. By contrast, suspects were more likely to make partial confessions and were less cooperative when the police officers employed an Evidence-focused style.     

The Structure of Indigenous Food Crop Markets in sub-Saharan Africa: The Rice Market in Uganda

Using data obtained from a series of nation-wide market surveys in Uganda, this article attempts to document and assess the domestic rice market at all stages in the post-harvest marketing chain from the farm gate to metropolitan area retail outlets. The criteria used are quantities marketed, prices, marketing margins, marketing costs and net returns to traders. The results show that the regional rice markets are integrated into the national market and that on average little surplus is left for rice traders at all market stages if marketing costs are accounted for. The spontaneously developed indigenous crop market works reasonably well.     

<Emphasis Type="Italic">Society’s</Emphasis> Books of Note

Society’s Books of Note

Society’s Books of Note January/February 2012     

D5S818 Typing Discrepancy Between PowerPlex® Fusion and Other STR Kits Including GlobalFiler® Caused by a One‐base Deletion in 31 Nucleotides Upstream of the Repeat Region

Short tandem repeat (STR) typing is widely used in forensic investigation. When the same DNA sample is analyzed with different STR typing kits, a typing discrepancy is occasionally observed. In this study, we examined the cause of a typing discrepancy in a sample at D5S818 locus. This sample was designated as 10, 12 using Identifiler^®, Identifiler^® Plus, GlobalFiler^®, PowerPlex^® 16HS, and PowerPlex^® 18D, but as 9.3, 12 using PowerPlex^® Fusion. Sequencing results indicated that the shorter allele in the sample had a deletion (U31Tdel) at 31 nucleotides upstream of the repeat region (AGAT)₁₀. This deletion was located in the binding site of the published D5S818 forward primer in PowerPlex^® 16 and was only 9 and 11 nucleotides downstream of our estimated 5′ end position of D5S818 forward primer in GlobalFiler^® and PowerPlex^® 18D, respectively. We also examined the effect of primer length on the heterozygous peak balance in this sample.     

World heritage and the local politics of memory: the Miike coal mine and fu no isan

Abstract

This article investigates the complex relations between heritage and memory through an analysis of the UNESCO World Heritage ‘Sites of Japan’s Meiji Industrial Revolution’ and its component Miike coal mine. When discussing this World Heritage Site, scholars and the media have focused on the diplomatic controversy over the history of forced labour between Japan and South Korea, interpreting it as a new example of a dispute over history in East Asia. However, this understanding oversimplifies the relations between heritage and memory. Based on fieldwork research and documentary analysis, this article investigates the diversity and complexity of the collective memory of Miike coal mine from a local perspective. The results show that there is a sharp dissonance between the World Heritage story and some of the ex-miners’ memories, which focus on the negative past, fu no isan. Documentary analysis shows that fu no isan has two different but closely related meanings: negative legacy and negative heritage. Fieldwork research reveals that the various commemorations of fu no isan in the local community, mainly based on ex-miners’ social networks, constitute a form of vernacular memory, independent from the official memory of the World Heritage. Finally, I conclude that heritage can be public memory, rather than just official memory, as long as it is open to plural memories of the past.