Spectroscopic determination of skin viability. A predictor of postmortem interval

We have demonstrated that skin viability decreases at a measurable rate following death in an animal model. The decreased skin viability was measured by fluorescein diacetate and ethidium bromide using fluorescence emission spectroscopy. There is significant decrease of the fluorescence intensity of the fluorescein diacetate assay between the 1-4 h, the 6-24 h, and the >40 h time points postmortem. For times between 6-24 h and >40 h postmortem the ethidium bromide assay showed consistent and significant increases in signal. The fluorescence measurements in this study showed that under the experimental conditions the time of death could be determined for <4, 6-24, and >40 hapotmotrem. The application of these assays in the field will require further study of the environmental factors.     

What Ministers Do

Having been a minister in three different departments for a period of over nine years (1999–2008), during the Blair and, briefly, the Brown governments, I thought it might be useful to reflect on what ministers actually do, their functions and how they spend their days. In thinking about what I did as a minister, I have avoided going back to some of the classic constitutional texts on what ministers supposedly do, how they are accountable and such like. I am sure there is good literature on this from political scientists and constitutionalists, but I have avoided influencing my own views and approach by consulting these texts.