Reliable, sensitive, rapid and quantitative enzyme-based assay for gamma-hydroxybutyric acid (GHB)

Several assays for gamma-hydroxybutyrate (4-hydroxybutyrate, GHB) have been developed based on the enzyme gamma-hydroxybutyrate dehydrogenase (GHB-DH). Enzymatic oxidation of GHB by NAD+ is coupled to diaphorase-mediated reduction of pro-dye to yield colored product. GHB-DH from Ralstonia eutropha was cloned and expressed as a stable fusion protein easily purified by affinity chromatography. Quantitative initial velocity and endpoint versions of the assay in solution are described. Michaelis-Menten parameters for oxidation of GHB and ethanol were estimated. A semi-quantitative "dipstick" version of the assay on paper also is described. Both solution endpoint and "dipstick" assays are sensitive to about 0.05 mg GHB/mL using 10 microL of sample. Ethanol at concentrations possible in urine and agents used to stabilize physiological fluids for forensics analysis do not interfere significantly. The "dipstick" assay also allows detection of GHB in alcoholic beverages after evaporation of about one-fourth drop of beverage before testing. The enzymatic assay for GHB is reliable, sensitive, inexpensive and rapid.     

Postmortem citalopram concentrations: alone or along with other compounds

Citalopram, an antidepressant whose use has become more widespread in Spain in recent years participates directly and indirectly in the lethal mechanism in voluntary and involuntary poisonings. There were 30 cases of autopsies in the Madrid region where citalopram and other psychoactive substances (psychotropic drugs, alcohol, opiates) were detected in the corpses. The postmortem citalopram levels in relation to the manner and mechanism of death were evaluated, and a significant difference between the toxic and nontoxic cases (p < 0.01) was found. We studied the citalopram blood levels alone and along with other psychoactive products, and these cases were then further divided into those where the compounds were at deadly levels and those which were not. We found a range of citalopram levels between 0.37 and 0.83 microg/mL in which some cases were associated with citalopram toxicity and others were not. Citalopram blood levels of less than 0.35 microg/mL did not lead to fatal poisoning when it was the sole substance detected.     

Chilean women and the UN decade for women

This article analyses two aspects of the reality of Chilean women during the United Nations Decade of Women: political participation and labour force participation. This analysis takes into account some of the proposals of the Regional Plan of Action for the Integration of Women into Latin American Economic and Social Development as well as the consequences of the political, cultural and economic model established by the Chilean military government. The effects of the Decade of Women on Chilean women is not analysed primarily because the specific proposals contained in the Plan of Action are based on the prerequisites of development and participation—which are not applicable to the Chilean case during this period.Notwithstanding the fact that all forms of popular participation have been effectively eliminated, there have emerged women's organizations whose concerns include calling into question the oppression of women on the basis of sex. With respect to labour, women's participation in the labour force has increased. However, this increase is not a response to improved opportunities or a more equalitarian treatment of women workers, but rather as the consequence of elevated unemployment rates especially in lower class families where women have greater access to certain kinds of marginal employment.On a final note, the article also includes a discussion of recent trends in research on women, trends which provide a challenge to the more traditional forms of research.     

Investing in international security: rising powers and organizational choices

How do rising powers choose to allocate their finite resources among the multiple global and regional security organizations? Building on the literatures on forum shopping and rising powers, we argue that the different organizational investment choices of rising powers are explained by varying regional ideational affinities. Organizational settings have ideational foundations that can look very different from region to region. We argue that regional ideational affinity leads rising powers to invest in regional rather than global organizations. However, if the ideational composition of the region is highly diverse, global organizations are a better vehicle to accommodate rising powers’ emergent ambitions. To demonstrate our argument, we examine the choices of Brazil and South Africa in terms of their material and ideational investments in regional and global organizations.     

Y-chromosome STRs in an Antioquian (Colombia) population sample

Haplotype data were obtained from a sample of 777 unrelated male individuals from Antioquia Department (Colombia), for eight Y-chromosome STRs (DYS19, DYS385, DYS389 I, DYS389 II, DYS390, DYS391, DYS392 and DYS393). A total of 442 different haplotypes were identified of which 334 were represented only once in the database and the most frequent haplotype was found in 32 individuals. A high haplotype diversity was found (99.45%). Genetic distances were calculated using previously published haplotype data and the lowest values were found for the comparisons with samples of lberian origin.     

EXCLUSIONARY PRICING AND CONSUMERS HARM: THE EUROPEAN COMMISSION'S PRACTICE IN THE DSL MARKET

The assessment of exclusionary abuses by dominant firms is byno means an easy task. This is particularly true for pricingabuses, as there is no clear-cut way of distinguishing a pricecut that is abusive from one that instead is pro-competitive.We argue that there are several shortcomings in the way theEuropean Commission as assessed exclusionary pricing abusesin two important decisions concerning Internet access servicemarkets: Deutsche Telekom AG and Wanadoo Interactive. Firstof all, in computing downstream costs to apply the price squeezetest the incumbent's economies of scale and unavoidable costsshould be factored in, as the test is only meant to establishwhether an as-efficient competitor has been unlawfully foreclosed.Secondly, since the price squeeze test provides only for a necessarycondition for predation to occur, it is also necessary to provethat the recoupment of initial losses is a plausible scenarioso that the conduct under examination may indeed turn out tobe harmful to consumers. This is all but a trivial task, asthere are some sources of endogeneity that may cause a radicalchange in the underlying market structure. Particularly, inmarkets at early stage of development, entry barriers that mayfacilitate the recoupment of initial losses ex-ante may causea change such that market structure no longer supports recoupmentex-post.     

Allele distribution of three X-chromosome STR loci in an antioquian population sample

The X-linked short tandem repeats (STR) markers have proven to be very useful tools for paternity testing when the disputed child is female. The aim of this study was to describe the polymorphism of three X-chromosomal STR loci (DXS6797, DXS6800 and HPRTB) in an Antioquian (Colombian) population sample. PCR products were separated in 4% acrylamide-bis-acrylamide denaturing gels followed by silver staining. Allele size determination and genotyping were performed according to recommendations of the DNA Commission of the International Society of Forensic Genetic using the allelic ladder manufactured at home and based on DNA controls including K562 and 9947A (Promega). Gene frequencies were calculated using ARLEQUIN version 3.11. Population genetic data were obtained by analyzing 127-400 unrelated males and 135 unrelated females from Antioquian (Colombian) population. Distribution of the allele frequencies of these systems for Antioquia population is similar to the European populations.     

Analysis of 16 Y-STRs in a sample of Colombian males

We studied and stablished a data base of 16 Y-STR (DYS19, DYS385, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, DYS439, DYS460, DYS461, GATA-A10, GATA-H4 and DYS635) in 133 unrelated Colombian males. The haplotype diversity was 0.9997 ± 0.0010. One hundred thirty one different haplotypes were found and only one was found three times. Good allelic representation in all the systems was observed, between 3 and 7 alleles, with the exception of the DYS385 with 35 allelic classes. DYS385 displayed the greater genic diversity (0.9012), whereas the system that displayed the smaller (0.4570) was the DYS393. This approach represents a very powerful tool for individual identification and paternity testing in forensic genetic.     

Genetic Polymorphisms of four X-STR Loci: DXS6797, DXS6800, HPRTB and GATA172D05 in a Peruvian population sample

The X linked short tandem repeats (STR) markers have proven to be very useful tools for paternity testing when the disputed child is female. The purpose of this study was to describe the genetic polymorphism of four X-chromosomal STR loci (DXS6797, DXS6800, HPRTB and GATA172D05) in a Peruvian population sample and evaluate their efficiency in forensic practice and paternity testing.