Complex chemical and toxicological analysis of prosidol

Methods for measuring a Russian narcotic analgesic prosidol are suggested: thin-layer chromatography, microcrystalloscopy, gaseous chromatography with ionization flame detector, gas chromatography with mass selective detector, ultraviolet spectroscopy, high-performance liquid chromatography with ultraviolet detector, proton nuclear magnetic resonance used in chemical toxicological analysis, pharmacological analysis, and investigation of material evidence pieces.     

Detection of cannabinoids in urine

Described in the paper are results of comparative examination of urine by the methods of immunochromatographic express analysis (ICA), fluorescence-polarization immune analysis (FIA) and of thin-layer chromatography (TLC) made for the purpose of detecting consumers of hemp products. A high specificity and a good sensitivity of the methods were demonstrated, which is a basis for using them as preliminary tests in detecting cannaboids and their metabolites in urine. The methods were evaluated quantitively and qualitatively versus the etalon method of chromato-mass-spectrometry. A possibility was pointed out to apply the methods of ICA and TLC as system analysis in the determination of 11-nordelta-9-tetrahydrocannabinol-9-carbonic acid in urine of marijuana users.     

Gas chromatography with mass-selective detector in testing blood for amitriptyline and nortriptyline

The article describes the method of simultaneous detection of amitriptiline and nortriptilin in cadaveric blood using gas chromatography with mass-selective detection. Preparation includes liquid-liquid extraction and derivation with trifluoroacetic anhydride. Nortriptilin threshold of detectability in the blood is 0.02 mcg/ml, amitriptilin one--0.05 mcg/ml. The range of detectability--0.05-3.0 mcg/ml for both compounds. Maximal error of the compounds detectability was under 12.9% for concentrations 0.10 mcg/ml and 10.6% for concentrations 2.0 mcg/ml. The method was tested on expert material in forensic chemical examinations.     

Chemical toxicological analysis of urinary cannabinoids by chromatography mass spectrometry

A chromato-mass-spectrometric method for identification of cannabinoids and 9-carboxy-11-nor-delta 9-tetrahydrocannabinol by their methyl derivatives is proposed. Use of alkaline hydrolysis in pretreatment of samples ensures the optimal conditions for complete isolation of 9-carboxy-11-nor-delta 9-tetrahydrocannabinol. Derivation with methyl iodide in absolute acetone in the presence of potassium carbonate was used. Preliminary conclusion on the presence of cannabinoids in the urine can be made from the results of enzyme immunoassay.     

Quantitative determination of strychnine in blood and urine by gas chromatography with mass-selective detector

A method for the quantitative determination of strychnine in biological fluids by gas chromatography--mass spectrometry is proposed. The preparation of samples for the analysis included extraction of strychnine from blood and urine with the use of AccuBond(II) EVIDEX cartridges for solid-phase extraction and SPEC MP3 disks respectively. The efficiency of extraction was estimated at 0.05 mg/l for blood and 0.02 mg/l for urine. The detection limit was 0.10 mg/l in blood and 0.05 mg/l in urine.     

Detection of blood opiates by fluorescence-polarization immunoassay

It was found possible to use fluorescence-polarization immunoassay (FPIA) in screening of opium drugs in blood and blood serum as a preliminary test. The results of the study of blood and serum samples by FPIA and chromato-mass-spectroscopy were compared. As a criterion of assessment of the preliminary study results we propose to use a cutoff point by serial production of the analysed blood samples of the threshold morphine concentrations (20 ng/ml for living subjects and 40 ng/ml--for corpses). The method of opiates screening in blood proposed is characterized by fast conduction, high specificity and precision, small amounts of the expert material.