In Situ Identification of Semen Stains on Common Substrates via Raman Spectroscopy,,

The spectroscopic identification of body fluids in situ is a major objective in forensic science. This approach offers the confirmatory, nondestructive, rapid, and on‐scene identification of various body fluids. Although Raman spectroscopy has shown tremendous promise toward this goal in prior proof‐of‐concept experiments, a significant challenge which still remains is substrate interference. Here, an approach for detecting semen stains in situ on various substrates using Raman spectroscopy is explored. Simulated semen evidence was prepared on skin, glass, and various fabrics. Raman data were accumulated from stains without any pretreatment using a common confocal mapping spectrometer using 785 nm laser excitation. The results demonstrate that the spectroscopic interferences encountered by substrates can be reduced and eliminated using a combination of existing subtraction techniques and chemometric models. Heterogeneous substrates proved most challenging, however, automatic subtraction treatment, and location of fluid hotspots was able to elucidate a clear spectroscopic signature of semen in every instance.     

Prevalence and distribution of premarital aggression among couples applying for a marriage license

This study used a modified version of the Conflict Tactics Scale to examine the prevalence of premarital aggression in a community sample of husbands and wives. The sample consisted of 625 couples participating in a longitudinal study of marital functioning and alcohol use. Consistent with past studies, the overall prevalence rate of husband-to-wife premarital aggression was 35%. Sociodemographic variables were associated with premarital aggression, with differences emerging primarily with regard to moderate aggression rather than mild aggression. Further, factors such as race, education, social class, cohabitation, and presence of children were independently associated with premarital aggression. This study is one of the first to examine premarital aggression in a large, heterogeneous sample of young husbands and wives at the same stage of relationship development. Future analyses will examine the prevalence, frequency, and continuity of husband and wife aggression over the first year of marriage.     

Regulators and Redskins

We examine the correlation between federal government activity and performance of the capital’s National Football League team, the Washington Redskins. We find a positive, non-spurious, and robust correlation between the Redskins’ winning percentage and bureaucratic output, measured by pages published in the Federal Register. Because the Redskins’ performance is prototypically exogenous, we give this result a causal interpretation and provide a plausible, causal mechanism: bureaucrats must make “logrolling” deals to expand their regulatory power, and a winning football team offers a shared source of optimism to lubricate such negotiations. We do not find the same correlation when examining congressional activity.     

The effect of sodium fluoride preservative and storage temperature on the stability of 6-acetylmorphine in horse blood, sheep vitreous and deer muscle

This study examined the in vitro stability of 6-acetylmorphine (6AM) in horse blood, sheep vitreous humour (VH) and homogenised deer muscle stored under different storage conditions. The stability of 6AM in horse blood is of interest because many toxicological laboratories utilise this matrix for the preparation of blood calibration and check standards and the latter are typically stored during routine use. Data on the storage stability of 6AM in human VH is extremely limited and no data has been reported in muscle. In the absence of human samples, 6AM stability was demonstrated in sheep vitreous and deer muscle. Blood and VH were stored with and without NaF at room temperature (RT), 4 and -18°C for 84 days. Muscle tissue homogenates were prepared in water with and without NaF and also in phosphate buffer (pH 6.0) containing NaF. Homogenates were stored for 31 days at RT, 4 and -18°C. Morphine and 6AM were extracted using SPE and quantified by GC-ion trap-MS/MS. In the absence of NaF, 6AM could not be detected after 7 and 14 days in blood stored at RT and 4°C, respectively. Although at -18°C 6AM was stable for 7 days (12% loss), only 54% was detected by day 84. The addition of NaF to horse blood increased 6AM stability substantially at every temperature. Further, the rate of degradation was found to be significantly slower in blood preserved with 2% NaF compared with 1% NaF (p=.05). 6AM was stable for the study period in preserved blood (1 and 2% NaF) stored at -18°C. For laboratories utilising horse blood in the preparation of standards, preservation with 1% NaF (minimum) and storage at -18°C is recommended. The addition of NaF to VH was essential for 6AM stability. Irrespective of temperature substantial losses (≥ 42%) were observed in unpreserved sheep VH by day 7. In preserved VH the concentration declined by only 22% on day 7 following storage at RT and no loss observed in VH stored at 4 and -18°C at the same time. In muscle, 6AM was stable for 7 days in preserved samples stored at RT and in all samples stored at 4°C and below. The addition of NaF increased the stability of 6AM substantially in muscle. The increased stability of 6AM in VH and muscle preserved with fluoride was attributed to inhibition of bacterial action and the subsequent reduction in the rate of putrefaction of these tissues.     

Complex Congenital Heart Malformation Including Transposition of the Great Vessels,Presenting as Sudden Unexplained Infant Death

Transposition of the great vessels (TGV) is a common congenital heart defect that is difficult to diagnose before birth. Antenatal diagnosis is associated with increased survival. Unusual features such as anomalous pulmonary artery origin may delay cyanosis, decreasing clinical suspicion. A three‐week old female infant who had never been cyanotic presented for forensic autopsy due to onset of unresponsiveness at home. History included risk factors for TGV and signs of heart enlargement that were not recognized during life. Cardiac pathology consultation identified D‐type TGV with additional rare anomalies. TGV may present as sudden unexplained infant death (SUID) for forensic autopsy if variant features prevent development of cyanosis. Cardiac pathology consultation is helpful in clarifying these features.     

Risky Firearms Behavior in Low-Income Families of Elementary School Children: The Impact of Poverty, Fear of Crime, and Crime Victimization on Keeping and Storing Firearms

This report examines possession and storage of firearms in low-income urban families with at least one child between 8 and 12 years of age. The data primarily consisted of responses to a survey administered to parents, but these data were supplemented by records obtained from discussion groups composed of children between 8 and 12 years of age. The data were collected from five low-income neighborhoods in a medium sized city in the Pacific Northwest as part of a larger study focusing on the presence of risk factors for substance abuse, violence, and gang activity. All five neighborhoods are known to be plagued by poverty, violence, substance abuse, and gang activity. To make our findings more understandable, we compared our findings from these neighborhoods to similar data from a middle-class neighborhood. Middle-class parents were twice as likely to have firearms in their homes, but were much less likely to keep them loaded and/or unlocked. High rates of victimization, fear of crime, self-protective behavior, and exposure to threats or attacks were associated with keeping firearms for protection and engaging in risky gun behavior in the home.