Advances in the diagnosis of wound vitality: a review

The diagnosis of the vital origin of wounds in many cases remains an unsolved problem for the forensic pathologist. Practical experience enables the expert to diagnose the vital or postmortem origin of wounds on the basis of macroscopic examination. In some cases, optic microscopy is used to confirm the diagnosis. In many other cases, additional more sensitive and specific markers of vitality are required. In the past 50 years, comprehensive research on this topic has resulted in a better understanding of the acute inflammatory reaction. The development and application of sensitive and specific markers through research in the areas of histochemistry, enzymology, and biochemistry has provided a partial solution to the problems involved in wound vitality diagnosis. A review of this challenging area of forensic pathology, including an explanation of these methods and markers, is presented in this paper.     

Quantification of forensic DNA from various regions of human teeth

When the use of traditional forensic identification methods such as fingerprints or dental radiographs is difficult or impossible, identification by DNA analysis has proven valuable. In situations such as explosions or airplane crashes, identification is even more difficult because human remains are often fragmented and may be commingled. Teeth are a useful source of DNA and can often survive extreme environmental conditions. However, teeth may be fragmented into several identifiable regions. Therefore it is important to determine if DNA is present in forensically significant yields in all regions of the tooth. The main objectives of this study were to determine which region(s) of the tooth contains quantifiable DNA, if all regions contain similar yields of DNA and whether there is enough DNA in all regions to justify DNA extraction from a found tooth fragment. Results demonstrate that there is sufficient quantity of DNA in the crown body, root body, and root tip to support DNA extraction. Additionally, the root body is the region with the highest yield of DNA. This information will aid forensic DNA analysts in producing a useful DNA profile in a timely and cost-effective manner.     

Identification of a skeleton using DNA from teeth and a PAP smear.

Identification of unknown living or deceased persons using dental treatment records is an established forensic technique. However, some cases remain unidentified, especially when antemortem dental records are not available for comparison to postmortem dental records. Cytological smears have been previously reported to be potential sources of DNA reference samples which can be compared to DNA recovered from found human remains. The case described here involves an adult skeleton which exhibited extensive, complex dental restorative treatment. A putative identification of the found skeleton as a missing woman was established using circumstantial evidence found at the scene. However, it became important to establish a positive identification using reliable scientific methods. When it was discovered that antemortem dental records were not available because the treatment was completed in another country and the treating dentist could not be found, cytological smears stained with Papanicolaou (PAP) stain obtained from the putative decedent's medical records were used as a reference DNA sample. DNA was recovered from the teeth of the skeleton using cryogenic grinding. Comparison of the genotypes resulted in the conclusion that the DNA originated from the same source. The use of PAP smears in this way is seen as a valuable resource in cases where positive identification using traditional dental and medical records is not possible.     

Solving certain dental records problems with technology--the Canadian solution in the Thailand tsunami response

Each natural or man-made disaster presents a different set of circumstances and, as a consequence, each event results in new challenges for response teams. The very large number of deceased victims in recent tragic events is certainly one of the enormous challenges that can tax many different resources for identification specialists. But there are other significant challenges. And during the response, some of these seem insurmountable. They can be solved if we embrace technologies outside the customary disciplines. The Canadian identification effort following the December 2004 tsunami involved responders from several disciplines. Each discipline faced challenges in many different and special areas that were difficult and in some cases previously unseen. This paper presents examples of the ways the Canadian identification team used Internet technology to solve problems that were encountered when it became necessary to duplicate victims' dental, medical and personal records, and transport these records halfway around the world. Digital images of the records were saved at high resolution in multi-page PDF files. These images were made available to on-site personnel using an encrypted, password-secured website.     

Management and safety of a medical mission: occupational hazards of volunteering

Medical aid missions involve travel to conflict or danger zones, posing safety risks in addition to the usual occupational risks arising from daily medical work. The note reports on a volunteer mission, using personal reports, anecdotal experiences, and the formal annual report to undertake an assessment similar to worksite assessments for hazards and control measures. Hazards were found to be prevalent, including physical noise and heat, infectious exposure from patients and local vectors, poor water sanitation, and psychosocial stress from unfamiliar environments and large patient numbers with limited means. Implementable preventative measures include increasing awareness with appropriate protective equipment usage and safety guidelines. Mission planning and management should also involve occupational health input.     

The European Court, National Judges, and Legal Integration: A Researcher's Guide to the Data Set on Preliminary References in EC Law, 1958--98

We hope to stimulate more systematic research on all areas of legal integration by making available for free and open use a comprehensive data base on preliminary references in EC law. The Data Set, which has been under construction since 1996, is now online at various websites. The data are not publicly accessible elsewhere. In this article, we provide a brief summary of the data base and its potential uses. We begin by introducing the main features of the Data Set. We then discuss some of the dynamics of legal integration in light of our analyses of the data.     

The Death of Freddie Gray and Its Impact on Homicides in Baltimore and Maryland

This study evaluated the impact that Freddie Gray’s death on April, 19, 2015, had on homicides within Baltimore, Prince George’s County, and the rest of Maryland. The numbers of these deaths were compared in the 2 years prior and after his death. The average number of homicide per month in Baltimore and Prince George’s County, but not the rest of Maryland, showed a statistically significant increase in the 2 years after his death, with a sudden spike in homicides in Baltimore immediately following his death. Spatial density (“heat”) maps of homicides showed an increase in clustering of deaths after Freddie Gray’s demise in portions of Baltimore, particularly near his arrest site. Hot spot maps showed a massive expansion of statistically significant clusters of homicides in areas of West and East Baltimore after his death. An emerging hot spot analysis revealed consecutive hot spots in large areas of West Baltimore with new hot spots prominent along the southern edge of this area. A review of the history of Baltimore, as well as discussions with Baltimore homicide detectives, revealed that prior socioeconomic and political trends likely primed Baltimore for the protests and violence that occurred after Freddie Gray’s death. It is probable that a perceived “overcharging” of officers involved in his death by the State’s Attorney resulted in a “pullback” from policing that directly led to increased homicides in Baltimore.