Identification of DNA of human origin based on amplification of human-specific mitochondrial cytochrome b region

Species-specific differences in a non-polymorphic region of the mitochondrial cytochrome b gene appear to be large enough to allow human-specific amplification of forensic DNA samples. We therefore developed a PCR-based method using newly designed primers to amplify a 157-bp portion of the human mitochondrial cytochrome b gene. The forward and reverse primers were designed to hybridize to regions of the human mitochondrial cytochrome b gene with sequences differing from those of chimpanzee by 26% (7 bp/27 bp) and 26% (6 bp/23 bp), respectively. Using this primer pair, we successfully amplified DNA extracted from blood samples of 48 healthy adults. All these human samples produced a single band of the expected size on agarose gel electrophoresis, and the sequence of the single band was shown to be identical to that of the target region (157 bp) by sequence analysis. On the other hand, no visible bands were amplified from DNA extracted from blood samples of animals including non-human primates (chimpanzee, gorilla, Japanese monkey, crab-eating monkey) and other species (cow, pig, dog, goat, rat, chicken and tuna). Thus, DNA producing a single band following PCR amplification using this primer pair can be reasonably interpreted as being of human origin. In addition, aged biological specimens comprising bloodstains, hair shafts and bones were successfully identified as being of human origin, illustrating the applicability of the present method to forensic specimens.     

Alcohol addiction of methamphetamine abusers in Japan.

Fifty-eight methamphetamine (MAMP) abusers were surveyed to demonstrate the types of nature of MAMP abuse. In-depth interviews using psychophysiological techniques assessed the subjects to be truthful or deceptive about their abuse histories. Research diagnostic criteria determined the subjects clinical symptoms. The results show that three factors characterize MAMP abuse: significant correlation was found between the years of abuse; the manifestation of somatic or mental disorders or both, and alcohol (Alc) addiction. MAMP abusers who were nondrinkers or light drinkers developed a tendency to dislike Alc after MAMP abuse, while there was no change in Alc consumption in heavy drinkers. The findings suggest that the combined abuse of MAMP and Alc aggravates somatic and mental disorders and that Alc plays an important role in the fatal effect of MAMP, especially from small doses. This provides support for the synergistic effects between MAMP and Alc. Coadministration of MAMP and Alc appears to produce long-acting and more complicated changes in the brain's neurotransmitter function.     

A new molecular approach to help conclude drowning as a cause of death: Simultaneous detection of eight bacterioplankton species using real-time PCR assays with TaqMan probes

We developed a novel tool for concluding drowning as a cause of death. We designed nine primer pairs to detect representative freshwater or marine bacterioplankton (aquatic bacteria) and then used real-time PCR with TaqMan probes to rapidly and specifically detect them. We previously cultured the genus Aeromonas, which is a representative freshwater bacterial species, in blood samples from 94% of victims who drowned in freshwater and the genera Vibrio and/or Photobacterium that are representative marine bacteria in 88% of victims who drowned in seawater. Based on these results, we simultaneously detected eight species of bacterioplankton (Aeromonas hydrophila, A. salmonicida; Vibrio fischeri, V. harveyi, V. parahaemolyticus; Photobacterium damselae, P. leiognathi, P. phosphoreum) using three sets of triplex real-time PCR assays and TaqMan probes labelled with fluorophores (FAM, NED, Cy5). We assayed 266 specimens (109 blood, 157 tissues) from 43 victims, including 32 who had drowned in rivers, ditches, wells, sea or around estuaries. All lung samples of these 32 victims were TaqMan PCR-positive including the lung periphery into which water does not readily enter postmortem. On the other hand, findings in blood and/or closed organs (kidney or liver) were PCR-positive in 84% of the drowned victims (except for those who drowned in baths) although the conventional test detected diatoms in closed organs in only 44% of the victims. Thus, the results of the PCR assay reinforced those of diatom tests when only a few diatoms were detectable in organs due to the low density of diatoms in the water where they were found. Multiplex TaqMan PCR assays for bacterioplankton were rapid, less laborious and high-throughput as well as sensitive and specific. Therefore, these assays would be useful for routine forensic screening tests to estimate the amount and type of aspirated water.     

Sanction Power, Jurisdiction, and Economic Policy-making: Explaining Contemporary Telecommunications Policy in Japan

Variation in policy-making is an important analytical issue in public policy analysis, but it has not been extensively discussed in the literature on Japanese politics and political economy. Focusing on the interaction between state and societal actors, this article presents a causal argument to account for variation in Japanese economic policy-making. It is argued that variation in policy-making patterns is determined by the strength of a bureaucracy's sanction power and the exclusivity of its jurisdiction. This argument is elaborated through analysis of four related cases of Ministry of Posts and Telecommunications (MPT) policy-making: the privatization process of Nippon Telegraph and Telephone (NTT), the regulation of telecommunications after NTT privatization, the promotion of advanced telecommunications, and the reorganization of NTT. The causal framework is also applied to contemporary Ministry of International Trade and Industry economic policy-making to highlight the argument's preliminary applicability to Japanese policy-making. This analysis of Japanese policy-making lays a base for further analysis of variation in policy-making in general.     

Detection of marine and freshwater bacterioplankton in immersed victims: Post-mortem bacterial invasion does not readily occur

We previously applied our method of detecting marine or freshwater bacterioplankton (bacteria) in the blood of immersed victims as a marker of drowning. However, we did not confirm the absence of post-mortem bacterial invasion during immersion. Here we examined the nature of bacterioplankton in blood samples from 21 immersed and 4 non-immersed cadavers. We found only freshwater bacterioplankton in the blood of two victims that were retrieved from the sea or an estuary inhabited by marine bacterioplankton even though one victim was highly putrefied. The results of diatom testing suggested that these two victims had drowned in fresh or brackish water with low salinity and then flowed out to the estuary or the sea. Two others were submerged in water, but representative bacterioplankton were undetectable in their blood although one victim was highly putrefied. Autopsy findings and the results of diatom tests did not indicate that the cause of death was drowning. As in previous studies, we identified freshwater bacterioplankton in the blood of seven other victims that had drowned in freshwater, marine bacterioplankton in the blood of four victims that had drowned in seawater and none in four victims found on land that had died by means other than drowning. Bacterioplankton in the blood of drowned victims appears to reflect the type of water aspirated and blood does not become easily contaminated with bacteria post-mortem even in decomposed bodies.     

President Gloria Macapagal‐Arroyo's anti‐corruption strategy in the Philippines an evaluation

Fighting corruption has been one of the priority issues for President Gloria Macapagal‐Arroyo for the past five years. Faster‐paced and result‐oriented implementation of anti‐corruption projects did bring some tangible results. There are new laws and executive and administrative orders related to anti‐corruption which have been passed. New strategies during her second term such as strengthening anti‐corruption agencies, more social value formation at the national level, and the use of e‐procurement in the government system, show encouraging signs for success. However, despite the effort and achievement, the public perception of corruption and sincerity of the government performance in fighting corruption remains unchanged or has even worsened. Is it due to lack of political will? Does this mean that the government is losing the war on corruption? The answers are complex, but two important reasons are the slow progress to improve the country's economy and the ineffectiveness of the government which has resulted in a negative public perception. The government needs to gain more public confidence so that the anti‐corruption reforms will strengthen good governance and make it accountable.     

关于颜延之的《陶征士诔》

颜延之的《陶征士诔》是对陶渊明一生的总结和评价。虽然,颜延之对陶渊明的认识,特别是对他的创作是有所保留的;但陶渊明那种自然而然的状态,以及“好读书,不求甚解,每有会意,便欣然忘食”的潇洒,则让他感慨万端,不能忘怀。不仅如此,通过《陶征士诛》的写作,还可以进一步了解颜延之的情感经历、仕宦生涯与同时代的文风、追求,并由此洞见其精神与创作上的特点。     

Still bilateral after all these years: US-Japan trade negotiations in telecommunications

Telecommunications is a leading industry that occupies a significantpart of the contemporary economy and impacts economic developmentconsiderably. Since the 1980s, three major trade disputes intelecommunications – the NTT procurement, MOSS/Motorola,and NTT interconnection charge disputes – have developedbetween the Japanese and US governments. In responding to USpressures, the Ministry of Posts and Telecommunications (MPT)has generally been receptive to US demands and willing to containnegotiations to a bilateral format, unlike the Ministry of InternationalTrade and Industry, which has become resistant to US pressuresand active in utilizing multilateral arrangements. This papershows that explicit government involvement in the industry concerned,combined with strong US pursuit of negotiations, made it difficultfor MPT to resist US pressure and resulted in its enactmentof measures conciliatory toward the United States. These findingsare then applied to brief analyses of other trade disputes toshow the generalizability of the approach.     

Future targets for Asian regional integration

The central framework of Asian integration is ASEAN+3 and, since its first Summit meeting in 1997, it has advanced regional integration in East Asia. Based on the direct experience of the author, this article presents a critical assessment of the progress made over the past ten years and argues that the ‘Singapore Declaration’ of 2007 is not ambitious enough for the future. An East Asian customs union (EACU) and common regional market should be the next targets for trade integration. In terms of monetary integration, Asia should aim for de-dollarisation and to achieve this it is crucially important to create a regional Asian monetary system (AMS).

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