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Metal and metalloid multi-elementary ICP-MS validation in whole blood, plasma, urine and hair. Reference values 总被引:5,自引:0,他引:5
Goullé JP Mahieu L Castermant J Neveu N Bonneau L Lainé G Bouige D Lacroix C 《Forensic science international》2005,153(1):39-44
Four multi-elementary metal and metalloid quantification methods using inductively coupled plasma mass spectrometry (ICP-MS) were developed and validated in human whole blood, plasma, urine and hair by means of a single preparation procedure for each sample. The ICP-MS measurements were performed using a Thermo Elemental X7CCT series and PlasmaLab software without a dynamic reaction cell. With this procedure 27-32 elements can be simultaneously quantified in biological matrices: Li, Be, B, Al, V, Cr, Mn, Co, Ni, Cu, Zn, Ga, Ge, As, Se, Rb, Sr, Mo, Pd, Ag, Cd, Sn, Sb, Te, Ba, W, Pt, Hg, Tl, Pb, Bi, U. Whole blood, plasma and urine samples (0.4 ml each) were diluted with purified water, acid, triton X100 and butanol. Rhodium was used as internal standard. The urine sample results were corrected for enzymatic creatinine determination. Twenty-five milligrams hair samples were acid mineralized after a decontamination procedure and diluted as previously described for biological fluids. To be validated, each element had to show linearity with a correlation coefficient higher than 0.99. The intra-assay and inter-assay inaccuracy, measured as the variation coefficient, were below 5 and 10% respectively. Global performance was assessed by a quality control program. Our laboratory is a registered participant of the Institut National de Santé Publique du Québec (Sainte-Foy, Canada) inter-laboratory comparison program for whole blood, urine, and beard hair of non-occupationally exposed individuals spiked with selected elements. In our study multi-element metal and metalloid analysis was assessed for 27 elements in whole blood, 27 elements in plasma, 30 elements in urine and 32 elements in hair, from 0 to 25, or 250 to 1000 ng/ml, depending on the element. Quantification limits ranged from 0.002 ng/ml (U) to 8.1 ng/ml (Al) for whole blood, from 0.002 ng/ml (U) to 7.7 ng/ml (Al) for plasma, from 0.001 ng/ml (U) to 2.2 ng/ml (Se) for urine, and from 0.2 pg/mg (Tl) to 0.5 ng/mg (B) for hair. Normal values were determined in whole blood (n=100), plasma (n=100), urine (n=100), and hair (n=45) of healthy volunteers, leading to approximately 10,000 analyses. All results are presented and discussed. Clinical toxicology and forensic toxicology applications are also reported. ICP-MS has made significant advances in the field of clinical biology, particularly in toxicological analysis. This is due to the use of extremely effective equipment that permits better clinical and forensic toxicological analysis of metal and metalloid status of each individual patient. 相似文献
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STANDARD OF REVIEW IN WTO LAW 总被引:1,自引:0,他引:1
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Camille Boulagnon M.D. B.S. Nicolas Leveque Pharm.D. Ph.D. Fanny Renois Ph.D. Laurent Andreoletti Pharm.D. Ph.D. Paul Fornes M.D. Ph.D. 《Journal of forensic sciences》2012,57(6):1650-1655
Abstract: In March 2009, a new strain of influenza A/H1N1 virus was identified in Mexico, responsible for a pandemic. Worldwide, more than 13,500 patients died, most often from acute respiratory distress syndrome. Because sudden death cases were rare, involving mostly young apparently healthy persons, influenza A/H1N1 (2009)‐related deaths may be misdiagnosed, which can raise medico‐legal issues. Case history: we report on an unexpected out‐of‐hospital death involving a young male with no past medical history and no vaccination. Fever was his only symptom. Laboratory tests: histology showed patchy necrotic foci with mononuclear inflammation in the lungs. The heart was histologically normal, but virological analyses using molecular biology on frozen myocardial samples showed high virus load. In conclusion, this case report shows that influenza A/H1N1 (2009) virus can be a cause of sudden cardiac death in the young and demonstrates the importance of quantitative virological analyses for the diagnosis of myocarditis. 相似文献
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Nicolas Florquin 《European Journal on Criminal Policy and Research》2014,20(3):323-341
What drives the prices of arms and ammunition sold at illicit markets? Do the prices of illegal arms soar during episodes of marked insecurity, such as conflict onset? This article seeks to advance knowledge on the dynamics and determinants of weapons prices through the quantitative analysis of illicit arms market price data in Lebanon for the period February 2011 to September 2012. The article also examines the relationship between arms and ammunition prices in Lebanon, and reported conflict fatalities in Syria, as the period under study overlapped with the onset of conflict in the latter country. Key results include strong, statistically-significant correlations between the prices of arms and the prices of ammunition in Lebanon, as well as between the prices of arms and ammunition in Lebanon and reported conflict fatalities in neighbouring Syria. These findings highlight the value of monitoring illicit arms market prices, including prices for a diverse range of weapons and ammunition, to improve our understanding of both illicit markets and conflict dynamics. The strong correlations observed in the article also suggest that crowdsourcing methodologies used by organisations monitoring killings during the Syrian conflict can effectively capture variations in conflict intensity over time. 相似文献
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Philipp Lottholz Nicolas Lemay-Hébert 《Cambridge Review of International Affairs》2016,29(4):1467-1485
This article analyzes how different interpretations of Max Weber’s work on the state and legitimacy have materialized in contemporary research on—and practice of—international state-building. We argue that the currently prevailing neo-Weberian institutionalism in state-building theory and practice is based on a selective interpretation of the passionate and polemicist ‘politician’ Max Weber, whilst omitting almost entirely the wealth of thought on interpretivist method and the anti-foundationalist approach to social sciences that he has developed in his scholarly work. The neo-Weberian institutionalist approach thus focuses almost exclusively on state capacity and institutions. In contrast to this restricted approach, we will show how Weber’s work on the historical and cultural dimensions of legitimacy is instructive in understanding the emergence and consolidation of social orders. Research agendas embracing such perspectives offer a viable way forward from the securitized approach to state-building and international intervention, in the process moving beyond the neo-Weberian orthodoxy. 相似文献
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Understanding the Relationship between Attachment,Caregiving, and Same Sex Intimate Partner Violence
Despite the well documented prevalence of same sex intimate partner violence (SSIPV), its dynamics remain relatively poorly understood. Building on the established value of attachment theory’s contribution to understanding heterosexual intimate partner violence, we provide a detailed examination of the relationship between the attachment system and SSIPV, while broadening our scope to include the caregiving system. A total of 310 American and Canadian individuals involved in same sex romantic relationships participated in an online survey. Hierarchical regressions revealed that attachment and caregiving variables shared significant amounts of variance with SSIPV, with avoidance of intimacy and proximity making independent contributions. Based on significant overlap between the attachment and caregiving dimensions, subsequent analyses were conducted to provide composite variables used to predict SSIPV. 相似文献