Single Nucleotide Polymorphism Assay for Genetic Identification of Lophophora williamsii*

Lophophora is a member of the Cactaceae family, which contains two species: Lophophora williamsii and L. diffusa. Lophophora williamsii is an illegal plant containing mescaline, a hallucinogenic alkaloid. In this study, a novel method based on a single nucleotide polymorphism (SNP) assay was developed for identifying L. williamsii; this assay reliably detects SNPs within chloroplast DNA (rbcL, matK, and trnL-trnF IGS) and was validated for identifying Lophophora and L. williamsii simultaneously. The chloroplast DNA sequences from four L. williamsii and three L. diffusa plants were obtained and compared using DNA sequence data from approximately 300 other Cactaceae species available in GenBank. From this sequence data, a total of seven SNPs were determined to be suitable for identifying L. williamsii. A multiplex assay was constructed using the ABI PRISM^® SNaPshot™ Multiplex Kit (Applied Biosystems, Forster City, CA) to analyze species-specific SNPs. Using this multiplex assay, we clearly distinguished the Lophophora among 19 species in the Cactaceae family. Additionally, L. williamsii was distinguished from L. diffusa. These results suggest that the newly developed assay may help resolve crimes related to illegal distribution and use. This multiplex assay will be useful for the genetic identification of L. williamsii and can complement conventional methods of detecting mescaline.     

Do Political Similarities Facilitate Interlocal Collaboration?

This research examines the extent to which political similarities—that is, homophily between political actors at the local level—affect patterns of interorganizational collaboration in an emergency response situation. While the field of emergency management has focused on implementation‐oriented arrangements among key stakeholders, few studies have systemically investigated the creation and development of interorganizational collaborations led by political actors, especially following catastrophic events. The analysis reveals that a dyadic tie with political homophily boosts local responders’ ties with other agencies during emergencies. Findings indicate that political solidarity, formulated by chief elected officials of municipalities and council members, can broaden the scope of interorganizational collaboration by mitigating institutional collective action problems at the local level. This research presents a critical recommendation for emergency managers that interlocal collaboration for timely response to a disaster is attributable to political similarities that facilitate frequent interlocal interactions through formal and/or informal agreements.     

Exploring the Determinants of the Entry and Exit of Ministers in Korea: 1980–2008

The entry and exit of ministers has been of primary interest to students of political science and public management in Western countries. Responding to the lack of research on the entry and exit of ministers in non‐Western countries, this article examined determinants of both the entrance and exit of ministers in Korea from the life cycle point of view based on the Korean Ministerial Database from 1980 to 2008. We argued that as the Korean presidency shifts from an imperialistic to a democratic presidency, ministerial appointments in Korea also seem to shift from an expertise‐focused to a politics‐focused approach. Likewise, the primary resignation reason also shifts from policy failure to political reasons. We also argued that Korean presidents use their power to reshuffle cabinet ministers too often for their political interests. As a result, Korean ministers spend too little time in post; average tenure is now down to about one year. These short terms in office dilute a minister's ability to dictate departmental policy.     

An Empirical Analysis on the Determinants of the Length of Ministerial Tenure in Korea, 1980–2008

ABSTRACT

The study of the length of ministerial tenure has received some attention by scholars of public management in Western countries. Responding to the lack of empirical research on ministerial duration in non-Western countries, this article empirically examines the determinants of ministerial duration based on the Korean Ministerial Database from 1980 to 2008. The empirical findings are as follows. First, being a female minister decreases the probability of stepping down by 1.78 times compared to a male minister. Second, political democratization after 1987 drastically increases the probability of ministerial stepping down by 3.46 times. Third, confirmation hearings after 2005 decrease the probability of ministerial stepping down by 0.53 times. Based on these empirical findings of the analysis, we can identify distinctive characteristics of ministerial duration in Korea. We argue that as the Korean political system shifts from military or authoritarian rule to democratic rule after 1987, a single five-year presidential term may set a political environment for frequent changes of ministers to allocate political spoils.     

Besprechungen

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Haplotype diversity in mitochondrial DNA hypervariable region I, II and III in northeast China Han

Sequence polymorphism of the mitochondrial DNA (mtDNA) control region, hypervariable regions HVR I, HVR II and HVR III, from 51 unrelated China Han (Yan Bian area) were determined by PCR amplification and cycle sequencing.     

Exploring the forensic use of the emotional recognition test (ERT)

General "hypo-emotionality" has now become a broadly accepted concept as one of the conspicuous psychological characteristics of repetitive, violent offenders. Numerous psychophysiological studies have verified this premise. The current study sought to examine the applicability of the Emotional Recognition Test (ERT) in a sample of 85 Korean inmates in a maximum-security prison. Two additional criterion groups were included for comparison: normal controls and schizophrenic inpatients. Because the ERT consists of problem-solving items, it is relatively free from response biases and is more efficient than measuring physiological responses. Results indicated that all criterion groups scored significantly differently on the ERT total and subscale scores. Additionally, similar to the physiological emotion-specific findings, emotion-specific subscales of the ERT indicated that offenders had significantly more difficulty in recognizing negative emotional stimuli, particularly sadness.     

Allele frequencies and genetic data of 16 highly polymorphic loci in Koreans

Allele and genotype frequencies for the 15 STR loci (FGA, vWA, MBP-L, MBP-H, HumTh01, D3S1358, D3S2406, D5S818, D7S820, D8S1179, D13S317, D18S51, D19S253, and D21S11) and two VNTR loci (D1S80 and D17S5) in a sample of unrelated Koreans were determined.     

Instruments for Exploring Organizational Culture: A Review of the Literature

Organizational culture is widely considered to be one of the most significant factors in reforming and modernizing public administration and service delivery. This article documents the findings of a literature review of existing qualitative and quantitative instruments for the exploration of organizational culture. Seventy instruments are identified, of which 48 could be submitted to psychometric assessment. The majority of these are at a preliminary stage of development. The study's conclusion is that there is no ideal instrument for cultural exploration. The degree to which any measure is seen as "fit for purpose" depends on the particular reason for which it is to be used and the context within which it is to be applied.