A concept study on identification and attribution profiling of chemical threat agents using liquid chromatography-mass spectrometry applied to Amanita toxins in food

Accidental or deliberate poisoning of food is of great national and international concern. Detecting and identifying potentially toxic agents in food is challenging due to their large chemical diversity and the complexity range of food matrices. A methodology is presented whereby toxic agents are identified and further characterized using a two-step approach. First, generic screening is performed by LC/MS/MS to detect toxins based on a list of selected potential chemical threat agents (CTAs). After identifying the CTAs, a second LC/MS analysis is performed applying accurate mass determination and the generation of an attribution profile. To demonstrate the potential of the methodology, toxins from the mushrooms Amanita phalloides and Amanita virosa were analyzed. These mushrooms are known to produce cyclic peptide toxins, which can be grouped into amatoxins, phallotoxins and virotoxins, where α-amanitin and β-amanitin are regarded as the most potent. To represent a typical complex food sample, mushroom stews containing either A. phalloides or A. virosa were prepared. By combining the screening method with accurate mass analysis, the attribution profile for the identified toxins and related components in each stew was established and used to identify the mushroom species in question. In addition, the analytical data was consistent with the fact that the A. virosa specimens used in this study were of European origin. This adds an important piece of information that enables geographic attribution and strengthens the attribution profile.     

Fighting corruption in public procurement in post-communist states: Obstacles and solutions

Anti-corruption efforts in Europe’s post-communist states have been less successful than anticipated. Criticism has been raised against the role of the international community in promoting anti-corruption programmes. Besides, such programmes have been deemed vague and “all-inclusive”. They have largely failed to address local factors “informing” corrupt behaviour in post-communist states, such as (a) negative perceptions of law, and (b) informal practice.‘ I'd be grateful if you could retain the original sentence as it is more precise.     

Children's reporting patterns after witnessing homicidal violence – the effect of repeated experience and repeated interviews

For both legal and clinical purposes, it is of importance to study children's memories and reports of stressful events. The present study investigated the reporting patterns of 83 children who had witnessed homicidal violence, which is considered to be a highly stressful experience. More specifically, we explored the possible effects of prior violence exposure and of repeated questioning on the amount of details reported. Results showed that the majority of children provided detailed reports about the homicidal violence they had witnessed, including details concerning what happened before, during, and after the violent act. The children provided detailed and vivid testimonies from their experiences, whether they witnessed the event for the first time or had prior experience of witnessing severe violence against the victim by the perpetrator. Children with no prior experience of repeated violence who underwent repeated interviews provided more details than those interviewed once. The present data indicate that children are competent witnesses when questioned in legal contexts after having been exposed to extremely stressful events. These findings have implications for research related to children's memories and reporting of traumatic experiences, as well as practical implications for future treatment and evaluation of children's testimonies.     

Recall of unpleasant emotion using memory-enhancing principles

Abstract

Two experiments are presented to examine the issue of memory for past emotions. Participants rated their emotional reactions on the occasion of watching a film, and in a subsequent session, they were asked to recall and rate the emotion as experienced when viewing it. In the first experiment, participants underestimated unpleasant emotion at recall, but their recall was improved with the presentation of a visual cue pertaining to the emotion-inducing film event. In the second experiment, participants were assisted in probing perceptual aspects of the event by means of memory-enhancing instructions underlying the Cognitive Interview. These participants consistently recalled emotional intensity, as compared to participants in the unassisted recall condition who underestimated prior emotion. This research suggests that ratings of prior emotion are easily influenced by features of the retrieval context, and that consistent recall may be achieved by memory-enhancing principles of the Cognitive Interview.     

Confessions: a Model of Officials' Perspectives on Accepting Gifts from Clients in Post-communist Europe

There is widespread criticism of the behaviour of junior officials in post-communist Europe where they are often accused of treating citizens unfairly and soliciting presents or bribes to solve citizens' problems. To get the perspective of officials themselves we interviewed 1307 junior officials and state employees in Ukraine, Bulgaria, Slovakia and the Czech Republic. This paper focuses on their willingness to accept gifts from clients, their confessions to having done so, and the factors that lead some officials to accept gifts while others do not – factors such as occupational bargaining power and the (related) frequency of offers from clients, economic pressures, national and institutional cultures, fear of punishment, and moral perspectives on charging clients for favours. Economic pressures make officials more inclined to feel that they 'could not afford to refuse' gifts, but this motivation is only weakly related to actual behaviour. Occupational bargaining power and the frequency of offers from clients make them more likely to 'welcome' gifts or accept them 'out of politeness'– and these less excusable motivations are more strongly related to actual gift-taking.     

A Prospective Study of the Potential Moderating Role of Social Support in Preventing Marginalization Among Individuals Exposed to Bullying and Abuse in Junior High School

Negative physical and psychological long-term consequences of abuse and bullying are well documented. It is reasonable to assume that abuse and bullying early in life also may have an impact on the ability to work and stay economically independent later in life, but such prospective studies are lacking. This study investigates the consequences of exposure to abuse and bullying in junior high school, as measured by receiving long-term social welfare benefits in young adulthood. In addition, it explores the potential protective role of social support. Self-reported data from 13,633 (50.3 % female) junior high school students were linked to registry data on their use of social welfare benefits from the age of 18 and for eight consecutive years. Cox regression analyses were applied to test the relationship between exposure to life adversities and the use of social welfare benefits, and the potential moderating role of social support. The analyses showed that individuals exposed to abuse and bullying had an increased likelihood of receiving social-welfare benefits compared with individuals not exposed to these types of abuse. Exposure to multiple types of abuse led to a higher likelihood of using social welfare benefits compared with single types of abuse and no abuse. The findings on the potential moderating role of social support were mixed, depending on the source of social support. Family support and classmate relationships were protective in reducing the likelihood of the use of social welfare benefits, whereas peer and teachers’ support showed inconsistent patterns. These results are promising in terms of preventing the long-term negative consequences of abuse and bullying.