Use of laser-scanning confocal microscopy in the detection of diagenesis in bone

This research demonstrates the value of laser scanning confocal microscopy (LSCM) as a research tool in osteological studies, and diagenetic studies in particular. LSCM combines properties of light and scanning electron microscopy using laser light to excite fluorophores throughout the z-axis, developing a 3-D image. Using differential staining and selecting for specific wavelengths of light, one can image targeted materials. This research is divided into two parts: visualizing bone structures such as proteins and their decompositional products and visualizing diagenesis. Part one of this study utilized pig bones as a means of testing the overall ability of LSCM to fluoresce bone. Twenty-three samples were imaged, including 13 samples from a decompositional study conducted 5 years previous, and 10 “fresh” samples collected from a commercial butcher. This part of the study determined that protein and organic components of the bone could be fluoresced and diagenetic alteration could be imaged. The second part of the study used human samples as a means of imaging and mapping diagenetic alterations. The second part of the study used 13 samples, including 4 clinical, 7 ancient, and 2 modern controls. The pig study used Basic Fuchsin and SlowFade Gold stains, while the human study used toluidine blue. Images were also taken with unstained elements. The results of the non-human study found that a fresh bone fluoresced differently than that of a 5-year subset, while the results of the human study confirmed these findings and determined that the bone diagenesis can be mapped using LSCM.     

The future of crime data

Criminology lacks sufficient data for many types of crime that are of great concern to society. This lack of data poses significant problems for determining whether resources are adequate for responding to these crimes or whether programmatic, legislative, or target-hardening efforts to prevent or reduce their occurrence are effective. Inadequate data about crime also produces a selective and incomplete narrative about crime that makes it easier for political and vested interests to exploit public concerns about crime for their own ends. In this address, I discuss what is needed to resolve these gaps and the ways in which criminologists can support a significant expansion of the crime data infrastructure. Such work is necessary to help ensure the future relevance of criminological research.     

Paradoxical individualization: ideological polarization in the U.S. in historical-comparative and theoretical perspective

ABSTRACT

Beginning with Karl Marx, theorists have seen individual autonomy and ideology as opposing elements. Ideology was considered the product of mental, cultural or social constraints. People did not choose their world view – they fell victim to it. With the increase of individual autonomy, however, ideology would wither away. In the late 20th century, the advocates of individualization theory have similarly predicted the vanishing of clear-cut ideological divisions in a world in which any form of collective identity was difficult to sustain. Political eclecticism and the mixing of different political world views would become the new norm. Politics was now ‘beyond left and right’, as Anthony Giddens once famously argued. In my article, I show that this understanding of the relationship between individualization and ideological polarization is flawed. By discussing the extreme ideological polarization in the U.S., I disprove the notion that our need for ideology vanishes the more our freedoms expand. On the contrary, precisely the increase in individual autonomy – in terms of mobility patterns, media use and lifestyle differentiation – has helped to turn the country into a series of closed echo chambers and to deepen the ideological fault lines of American society.     

Lessons from International Multi-Option Referendum Experiences

Referendums are regularly criticised for reducing complex policy decisions to two maximally opposed options. This reduces opportunities for voter expression and can polarise debates. Alternative referendum designs which present more than two ballot options can offer innovative opportunities, but also raise new challenges. We can benefit by learning from previous experiences with multi-option referendum voting. Discussions of such experiences are rare and have often focussed on a limited number of cases. This article provides an overview of over 100 multi-option referendum experiences around the world. It discusses the topics on which they were held and the ballot options that were offered. It then analyses the variety in ballot design in terms of questions posed and voting methods applied. Drawing on the experiences of multi-option referendums, the article concludes with lessons that can be learned in relation to initiating and designing these referendums.     

For Safety or Profit? How Science Serves the Strategic Interests of Private Actors

Science is central to the regulation of risk. But who provides the science on which risk regulations are based? Through an in-depth empirical analysis of domestic health and safety standards, this article shows how private actors use scientific information to acquire preferential outcomes. I develop a formal model delineating conditions under which firms will seek stricter standards on their own products, and I reveal how companies can acquire these outcomes through the strategic provision of information. To test the theory, I track changes to U.S. agrochemical standards over a two-decade period. I also introduce firm-level petition data and historical evidence to test the mechanism directly. My findings provide new insight into the strategies companies use to benefit from regulations, while also forcing us to reevaluate what it means for regulations to be based on science.     

Collective Bargaining during Times of Crisis: Recommendations from the COVID-19 Pandemic

The COVID-19 pandemic dramatically changed employment across sectors in 2020. This Viewpoint essay examines public sector labor relations during the pandemic and describes the impact bargaining process that is used to protect public employees. The authors draw on their own experience with impact bargaining negotiations and the public labor relations, conflict management, and civil service reform literatures to develop recommendations for public union labor leaders during times of crisis. They suggest that public unions have an important role in crisis management but must act strategically to develop good working relationships with leadership and successfully negotiate employee protections in uncertain times.     

Development of a Validity Scale for the Dissociative Experience Scale-Revised: Atypicality,Structure, and Inconsistency

Psychological Injury and Law - The Dissociative Experiences Scale (DES), the most commonly used scale for assessing dissociation across settings, lacks a validity scale. In this study, six methods...     

Youth Adversities Amplify the Association between Adult Stressors and Chronic Inflammation in a Domain Specific Manner: Nuancing the Early Life Sensitivity Model

There is strong evidence that chronic, systemic inflammation hastens onset of the diseases of old age that ultimately lead to death. Importantly, several studies suggest that childhood adversity predicts chronic inflammation. Unfortunately, this research has been plagued by retrospective reports of childhood adversity, an absence of controls for adult stressors, and a failure to investigate various competing models of the link between childhood adversity and chronic inflammation. The present study was designed to address these limitations. Using 18 years of data collected from 413 African Americans (58% female) included in the Family and Community Health Study, hierarchical regression analyses provided support for a nuanced early life sensitivity explanation for the link between early adversity and adult chronic inflammation. Controlling for health risk behaviors and adult SES, late childhood (ages 10–12) adversity amplified the association between adult adversity (age 29) and chronic inflammation. This interaction operated in a domain-specific fashion. Harsh parenting amplified the relation between intimate partner hostility and inflammation, whereas early discrimination amplified the relation between adult discrimination and inflammation. These findings suggest that individuals may be primed to respond physiologically to adverse adult circumstances that resemble those experienced earlier in life.

     

Dual-Credit Courses and the Road to College: Experimental Evidence from Tennessee

Dual-credit courses expose high school students to college-level content and provide the opportunity to earn college credits, in part to smooth the transition to college. With the Tennessee Department of Education, we conduct the first randomized controlled trial of the effects of dual-credit math coursework on a range of high school and college outcomes. We find that the dual-credit advanced algebra course alters students’ subsequent high school math course-taking, reducing enrollment in remedial math and boosting enrollment in precalculus and Advanced Placement math courses. We fail to detect an effect of the dual-credit math course on overall rates of college enrollment. However, the course induces some students to choose four-year universities instead of two-year colleges, particularly for those in the middle of the math achievement distribution and those first exposed to the opportunity to take the course in eleventh rather than twelfth grade. We see limited evidence of improvements in early math performance during college.