猪日本乙型脑炎病毒NS1基因重组腺病毒的构建及表达

应用RT-PCR方法扩增出猪日本乙型脑炎病毒(JEV)的NS1基因,通过SalⅠ EcoRⅠ双酶切后把该基因插入到经过同样双酶切的穿梭质粒pDC315中。重组穿梭载体经过双酶切和PCR鉴定后进行测序,序列测定正确。将获得的重组穿梭质粒与腺病毒骨架质粒共转染293细胞后获得重组腺病毒。PCR鉴定及间接ELISA检测的结果证明所构建的重组腺病毒成功地表达了JEV的NS1。     

论"看"在日语中的运用

文中从中文的“看”分析出看字在日文中表达时的微妙区别,并根据它们的词性和日语的习惯表达说明了简单的看字的区别。     

论新时期寻根电影思潮的发展历程

新时期寻根电影对传统文化，或批判或皈依，都充溢着浓郁的文化意蕴。从1980年代的“电影民族化”大讨论开始，到90年代新民俗电影的兴盛，新时期寻根电影思潮的发展历程依稀可辨。80年代中期以后，电影界自觉地呼应文学界的文化寻根运动，一批寻根电影相继问世，展现了这一思潮的创作实绩。进入90年代以后，在主旋律电影和商业电影对艺术电影的挤压和吸附的生存困境中，电影依然充溢着绵延不断的寻根精神，一部分呈现出强烈的皈依趋向，另一部分如新民俗电影集中对传统文化进行批判，产生了广泛的影响。     

论日本女训中的儒家女性观

受中国女训影响,随着儒家思想的兴盛以及封建家制度的确立和完善,日本女训在近世得到了广泛的发展和普及。对男尊女卑等儒家女性观的吸收和借鉴是日本女训最为鲜明的特色之一,但这种借鉴并不是全盘照搬,而是根据日本的社会现状,有选择地加以模仿。探讨其中所体现的儒家女性观,有利于我们把握当时日本女性的生存状况及其对当今日本女性社会地位的影响。     

中日稻作起源研究回顾

1973年河姆渡遗址发现后,中日两国学者对稻作起源的研究有了突破性进展,对稻作起源年代的认定更为明确,尤其是两国学者对稻作起源区域的高度认同以及研究方法、内容上的互补,大大丰富了稻作文化的内涵。     

冷战结束后日本马克思主义研究综述

日本马克思主义在经受了苏东剧变所带来的世界反马克思主义浪潮的严峻考验后,逐渐复苏并呈现出蒸蒸日上的发展态势.本文对此进行了梳理,并对全球化理论、环境思想以及社会主义理论等日本马克思主义研究的理论热点进行了重点概述.     

Representations of the family in postwar British amateur film: family histories in the Lane and Scrutton collection at the East Anglian Film Archive

This article examines the construction of the postwar British family in amateur film with reference to the Sidney Lane and Cecil Scrutton collection held at the East Anglian Film Archive (EAFA), particularly the films covering 1948 – 1961. Heather Norris Nicholson argues that home movies contribute to ‘an understanding of leisure and visual-related practices of consumption as well as the social processes by which people came to give themselves, and others, identities’ in the mid-twentieth century (Nicholson, H. N. [2004]. At Home and Abroad with Cine Enthusiasts: Regional Amateur Filmmaking and Visualizing the Mediterranean, ca. 1928 – 1962’. Geojournal, 49, 323–333). By considering the social and historical contexts in which these home movies were produced, and using accompanying notes by one of the filmmaker’s sons, the leisure time films of Lane and Scrutton can be analysed in order to understand how the amateur cine hobby ideologically constructed family, community and national identity in postwar Britain. The images of Christmas parties, daytrips and holidays in these films reveal much about this particular family, and are therefore very illuminating to the social historian and film scholar of today.     

THIS WEEK

正A WAlK FoR PEACE ANd REMEMBRANCEQueen Margrethe II of Denmark walks across Peace Square during her visit to the Memorial Hall for the victims of the Nanjing Massacre by Japanese Forces in Nanjing,capital of east China’s Jiangsu Province,on April 27.     

Akutagawa Ryūnosuke's uncanny travels in Republican-era China

This study focuses on Akutagawa Ryūnosuke's Shina yūki (Travels in China): an account of a four-month journey through eastern, central, and northern China in the spring and summer of 1921. Due to Akutagawa's reputation as a writer and the account's vantage point on a transitional period in Japan's expansion abroad, Travels in China has traditionally enjoyed a prominent place in the canon of twentieth-century Japanese travel writing. What has received less attention, however, is the relation of the work to the rest of Akutagawa's literary corpus. In this paper, I situate Travels in China within the larger context of Akutagawa's ongoing interest in Chinese fiction and drama. Rather than reading Travels in China as a work of journalism, as Akutagawa initially invited his readers to do, I argue that the work is an extended exploration not only of the relationship between ‘New China’ and Akutagawa's beloved traditional Chinese culture, but also of the boundaries separating journalism, fiction, and other literary genres. Ultimately, I connect Travels in China to Akutagawa's later work: texts that similarly interrogate and deconstruct the distinctions between genres and modes of narrating experience.     

Spiritualism and modernism in the work of Kawabata Yasunari

Abstract

Kawabata Yasunari held a deep interest in the study of spiritualism from his very youth. He demonstrates his knowledge of the field in his writing in a variety of ways, particularly in the short stories he published during the prewar period. Thus far, scholarship has considered Kawabata's relationship with spiritualism primarily in terms of his biography: there has yet to be a satisfactorily rigorous study taking into account historical context and his use of spiritualism as novelistic technique. This article analyzes Kawabata and spiritualism with respect to modernism by looking at the short stories ‘The Full White Moon’, ‘Requiem’, and ‘Lyric Poem’. This analysis will also help illuminate the diversity of Kawabata's approaches to modernism.