三个单位点DNA杂交联合应用的研究

本文从一些多态位点中筛选出在中国人群中，对于同一种限制酶HaeⅢ酶解都能检出良好多态性的三个单位点探针（PMLJ14、PYNH24、α－globin－3’HVR）。对这三个位点的等位基因频率进行了调查．用DNA指纹自动识别系统进行了数据处理，各位点的数据如下：PMLJ14、杂合度94％，等位基因频率分布0．002～0．051；PYNH24：杂合度89％，等位基因频率分布0．003～0．152；α－globin-3’HVR：杂合度78％，等位基因频率分布0．003～0．077。分析15个家系，未见到变异发生，符合孟德尔遗传规律。这三个位点个人识别中的累加机率是：1．7×10-5～2．1×10-14。     

Congressional Attention and Opportunity Structures: The Select Energy Independence and Global Warming Committee

This article examines the role that overlapping committee jurisdictions play in fostering or hindering attention to environmental and energy issues in the U.S. Congress. The Select Energy Independence and Global Warming Committee, created by House Democrats in 2007 , and its chairman Rep. Ed Markey took advantage of opportunities within the existing jurisdictional arrangement to increase attention to these issues despite lacking any legislative authority. Data from the 110th and 111th Congresses show that the Select Committee held more hearings on climate change and alternative energy, and on environmental and energy policy generally, than any other committee. The committee also increased the range of sources informing debate on these issues by calling different witnesses than other committees. These findings have important implications for the relationship between institutional structure and agendas and for Congress' policy‐making role on energy and the environment.     

守正、发展与创新:“十四五”时期医疗保障的完善思路

人口结构、就业形态、新时代的健康政策与脱贫攻坚任务、技术发展等宏观环境,为“十四五”时期医疗保障的发展提供了机遇也提出了挑战。立足“十一五”到“十三五”医疗保障从无到有、从制度全民覆盖到实践全民覆盖、待遇水平持续提升、各项机制跨越式发展等发展脉络,“十四五”时期医疗保障的发展思路应定位于守正、发展与创新。第一,坚守公平共享与切实保障的发展理念;第二,回应健康需求与不平衡、不充分的医保制度现状,在待遇保障、筹资机制、支付方式、基金监管等方面补短板、强弱项;第三,直面新形势的新挑战,在新业态人员参保、大病保障、医疗保障与公共卫生资金统筹衔接等方面实现创新与突破。目标是在“十四五”时期实现一个全民享有、公平适度、法治规范、高效便捷、协同联动的医疗保障制度。     

Policy Adoption and Policy Intensity: Emergence of Climate Adaptation Planning in U.S. States

The United States is experiencing growing impacts of climate change but currently receives a limited policy response from its national leadership. Within this policy void, many state governments are stepping up and taking action on adaptation planning. Yet we know little about why some states adopt State Adaptation Plans (SAPs), while others do not. This article investigates factors that predict the emergence of SAPs, both in terms of policy adoption and policy intensity (goal ambitiousness). Applying the diffusion of innovation theory, I consider the relative influence of internal state characteristics, regional pressures, and test for conditional effects between government ideologies and severity of the problem. The results show interesting differences between predictors that influence policy adoption and ambitiousness. States are more motivated to adopt a policy when faced with greater climate vulnerability, have more liberal citizenry, and where governments have crossed policy hurdles by previously passing mitigation plans. The intensity of policies and goal setting, moreover, is more likely to be driven by interest group politics and diffuse through policy learning or sharing information among neighboring states in Environmental Protection Agency regions. These findings support an emerging scholarship that uses more complex dependent variables in policy analysis. These variables have the potential to differentiate symbolic from substantive policies and capture finer information about predictors of importance.     

论奸淫幼女犯罪的"明知"--与苏力教授商榷

奸淫幼女犯罪在主观方面必须具备“明知”和“故意”,即行为人主观方面必须明知奸淫的对象是不满14周岁的幼女。我国刑法中没有严格责任犯罪,奸淫幼女犯罪在理论上也不宜实行严格责任。完全不考虑男性行为人主观方面的故意和明知,仅以“幼女自愿的意思表示在法律上无效”为由就认定强奸罪成立,这是典型的客观归罪。     

鸭肠炎病毒UL6基因B细胞表位的预测及其主要抗原域的原核表达

采用DNAStar Protean程序,综合运用二级结构、亲水性、可塑性和抗原性指数等参数,对鸭肠炎病毒(DEV)CHv毒株UL6基因(GenBank登录号EF055890)的氨基酸序列进行了B细胞表位预测。结果显示,UL6蛋白羧基端第Thr507~Gln515、Thr521~Met529、Asp531~Phe539、Gly544~Asn562、Thr568~Gln585、Lys592~Val604、Ala681~Ala778和Tyr780~Lys790区域内含有优势B细胞表位。以DEVCHv毒株基因组作为PCR模板,利用Oligo6.0软件设计了1对引物,整体扩增涵盖上述具有优势表位的基因片段,将其亚克隆到原核表达载体pET-32a( )中,获得表达载体pET-32a-UL6M,再将其转化至E.coliRosetta(DE3),经IPTG诱导,外源基因获得了良好表达。以兔抗DEV多克隆血清进行Western-blot分析,抗血清可与该融合蛋白发生特异性反应。提示,该氨基酸片段可能含有软件预测的线性表位区。